dCAS9-VP64_GFP Citations (54)
Originally described in: Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F Nature. 2014 Dec 10. doi: 10.1038/nature14136. PubMed Journal
Articles Citing dCAS9-VP64_GFP
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| Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Jusiak B, Cleto S, Perez-Pinera P, Lu TK. Trends Biotechnol. 2016 Jan 22. pii: S0167-7799(15)00274-7. doi: 10.1016/j.tibtech.2015.12.014. PubMed |
Manipulating the Prion Protein Gene Sequence and Expression Levels with CRISPR/Cas9.
Kaczmarczyk L, Mende Y, Zevnik B, Jackson WS.
PLoS One. 2016 Apr 29;11(4):e0154604. doi: 10.1371/journal.pone.0154604. eCollection 2016.
PubMed
Associated Plasmids |
| Targeted HIV-1 Latency Reversal Using CRISPR/Cas9-Derived Transcriptional Activator Systems. Bialek JK, Dunay GA, Voges M, Schafer C, Spohn M, Stucka R, Hauber J, Lange UC. PLoS One. 2016 Jun 24;11(6):e0158294. doi: 10.1371/journal.pone.0158294. eCollection 2016. PubMed |
| Inhibition of histone deacetylase reduces transcription of NADPH oxidases and ROS production and ameliorates pulmonary arterial hypertension. Chen F, Li X, Aquadro E, Haigh S, Zhou J, Stepp DW, Weintraub NL, Barman SA, Fulton DJ. Free Radic Biol Med. 2016 Oct;99:167-178. doi: 10.1016/j.freeradbiomed.2016.08.003. Epub 2016 Aug 3. PubMed |
| Epigenetic Regulation of Interleukin 6 by Histone Acetylation in Macrophages and Its Role in Paraquat-Induced Pulmonary Fibrosis. Hu L, Yu Y, Huang H, Fan H, Hu L, Yin C, Li K, Fulton DJ, Chen F. Front Immunol. 2017 Jan 30;7:696. doi: 10.3389/fimmu.2016.00696. eCollection 2016. PubMed |
| Rational design of inducible CRISPR guide RNAs for de novo assembly of transcriptional programs. Ferry QR, Lyutova R, Fulga TA. Nat Commun. 2017 Mar 3;8:14633. doi: 10.1038/ncomms14633. PubMed |
| Wnt/Tcf1 pathway restricts embryonic stem cell cycle through activation of the Ink4/Arf locus. De Jaime-Soguero A, Aulicino F, Ertaylan G, Griego A, Cerrato A, Tallam A, Del Sol A, Cosma MP, Lluis F. PLoS Genet. 2017 Mar 27;13(3):e1006682. doi: 10.1371/journal.pgen.1006682. eCollection 2017 Mar. PubMed |
| RNA-guided transcriptional activation via CRISPR/dCas9 mimics overexpression phenotypes in Arabidopsis. Park JJ, Dempewolf E, Zhang W, Wang ZY. PLoS One. 2017 Jun 16;12(6):e0179410. doi: 10.1371/journal.pone.0179410. eCollection 2017. PubMed |
Discovery of stimulation-responsive immune enhancers with CRISPR activation.
Simeonov DR, Gowen BG, Boontanrart M, Roth TL, Gagnon JD, Mumbach MR, Satpathy AT, Lee Y, Bray NL, Chan AY, Lituiev DS, Nguyen ML, Gate RE, Subramaniam M, Li Z, Woo JM, Mitros T, Ray GJ, Curie GL, Naddaf N, Chu JS, Ma H, Boyer E, Van Gool F, Huang H, Liu R, Tobin VR, Schumann K, Daly MJ, Farh KK, Ansel KM, Ye CJ, Greenleaf WJ, Anderson MS, Bluestone JA, Chang HY, Corn JE, Marson A.
Nature. 2017 Sep 7;549(7670):111-115. doi: 10.1038/nature23875. Epub 2017 Aug 30.
PubMed
Associated Plasmids |
| CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation. Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M. Nat Methods. 2017 Oct;14(10):963-966. doi: 10.1038/nmeth.4430. Epub 2017 Sep 11. PubMed |
| Enhancer connectome in primary human cells identifies target genes of disease-associated DNA elements. Mumbach MR, Satpathy AT, Boyle EA, Dai C, Gowen BG, Cho SW, Nguyen ML, Rubin AJ, Granja JM, Kazane KR, Wei Y, Nguyen T, Greenside PG, Corces MR, Tycko J, Simeonov DR, Suliman N, Li R, Xu J, Flynn RA, Kundaje A, Khavari PA, Marson A, Corn JE, Quertermous T, Greenleaf WJ, Chang HY. Nat Genet. 2017 Nov;49(11):1602-1612. doi: 10.1038/ng.3963. Epub 2017 Sep 25. PubMed |
| Evaluating different DNA binding domains to modulate L1 ORF2p-driven site-specific retrotransposition events in human cells. Ade CM, Derbes RS, Wagstaff BJ, Linker SB, White TB, Deharo D, Belancio VP, Ivics Z, Roy-Engel AM. Gene. 2018 Feb 5;642:188-198. doi: 10.1016/j.gene.2017.11.033. Epub 2017 Nov 14. PubMed |
| Activation of the Anti-Aging and Cognition-Enhancing Gene Klotho by CRISPR-dCas9 Transcriptional Effector Complex. Chen CD, Zeldich E, Li Y, Yuste A, Abraham CR. J Mol Neurosci. 2018 Feb;64(2):175-184. doi: 10.1007/s12031-017-1011-0. Epub 2018 Jan 19. PubMed |
| Pinpointing recurrent proviral integration sites in new models for latent HIV-1 infection. Lange UC, Bialek JK, Walther T, Hauber J. Virus Res. 2018 Apr 2;249:69-75. doi: 10.1016/j.virusres.2018.03.007. Epub 2018 Mar 14. PubMed |
| CRISPR/Cas9-Based Cellular Engineering for Targeted Gene Overexpression. Osborn MJ, Lees CJ, McElroy AN, Merkel SC, Eide CR, Mathews W, Feser CJ, Tschann M, McElmury RT, Webber BR, Kim CJ, Blazar BR, Tolar J. Int J Mol Sci. 2018 Mar 22;19(4). pii: ijms19040946. doi: 10.3390/ijms19040946. PubMed |
Mouse medulloblastoma driven by CRISPR activation of cellular Myc.
Vo BT, Kwon JA, Li C, Finkelstein D, Xu B, Orr BA, Sherr CJ, Roussel MF.
Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1038/s41598-018-24956-1.
PubMed
Associated Plasmids |
| lncRNA PAPAS tethered to the rDNA enhancer recruits hypophosphorylated CHD4/NuRD to repress rRNA synthesis at elevated temperatures. Zhao Z, Senturk N, Song C, Grummt I. Genes Dev. 2018 Jun 1;32(11-12):836-848. doi: 10.1101/gad.311688.118. Epub 2018 Jun 15. PubMed |
| CRISPRa-mediated NEAT1 lncRNA upregulation induces formation of intact paraspeckles. Yamazaki T, Fujikawa C, Kubota A, Takahashi A, Hirose T. Biochem Biophys Res Commun. 2018 Sep 26;504(1):218-224. doi: 10.1016/j.bbrc.2018.08.158. Epub 2018 Sep 1. PubMed |
| Enhanced intrinsic CYP3A4 activity in human hepatic C3A cells with optically controlled CRISPR/dCas9 activator complex. Han S, Wei S, Wang X, Han X, Zhang M, Su M, Li Y, Guo J, Zeng W, Liu J, Gao Y, Shen L. Integr Biol (Camb). 2018 Dec 19;10(12):780-790. doi: 10.1039/c8ib00109j. PubMed |
| PI3K Inhibition Activates SGK1 via a Feedback Loop to Promote Chromatin-Based Regulation of ER-Dependent Gene Expression. Toska E, Castel P, Chhangawala S, Arruabarrena-Aristorena A, Chan C, Hristidis VC, Cocco E, Sallaku M, Xu G, Park J, Minuesa G, Shifman SG, Socci ND, Koche R, Leslie CS, Scaltriti M, Baselga J. Cell Rep. 2019 Apr 2;27(1):294-306.e5. doi: 10.1016/j.celrep.2019.02.111. PubMed |
Cell-specific CRISPR-Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins.
Hoffmann MD, Aschenbrenner S, Grosse S, Rapti K, Domenger C, Fakhiri J, Mastel M, Borner K, Eils R, Grimm D, Niopek D.
Nucleic Acids Res. 2019 Apr 15. pii: 5458120. doi: 10.1093/nar/gkz271.
PubMed
Associated Plasmids |
Targeted Transgene Activation in the Brain Tissue by Systemic Delivery of Engineered AAV1 Expressing CRISPRa.
Lau CH, Ho JW, Lo PK, Tin C.
Mol Ther Nucleic Acids. 2019 Jun 7;16:637-649. doi: 10.1016/j.omtn.2019.04.015. Epub 2019 Apr 23.
PubMed
Associated Plasmids |
| Identification of Renal Long Non-coding RNA RP11-2B6.2 as a Positive Regulator of Type I Interferon Signaling Pathway in Lupus Nephritis. Liao Z, Ye Z, Xue Z, Wu L, Ouyang Y, Yao C, Cui C, Xu N, Ma J, Hou G, Wang J, Meng Y, Yin Z, Liu Y, Qian J, Zhang C, Ding H, Guo Q, Qu B, Shen N. Front Immunol. 2019 May 3;10:975. doi: 10.3389/fimmu.2019.00975. eCollection 2019. PubMed |
| Human evolved regulatory elements modulate genes involved in cortical expansion and neurodevelopmental disease susceptibility. Won H, Huang J, Opland CK, Hartl CL, Geschwind DH. Nat Commun. 2019 Jun 3;10(1):2396. doi: 10.1038/s41467-019-10248-3. PubMed |
| Regulation of CHD2 expression by the Chaserr long noncoding RNA gene is essential for viability. Rom A, Melamed L, Gil N, Goldrich MJ, Kadir R, Golan M, Biton I, Perry RB, Ulitsky I. Nat Commun. 2019 Nov 8;10(1):5092. doi: 10.1038/s41467-019-13075-8. PubMed |
| TUG1 Is a Regulator of AFP and Serves as Prognostic Marker in Non-Hepatitis B Non-Hepatitis C Hepatocellular Carcinoma. Lin YH, Wu MH, Huang YH, Yeh CT, Lin KH. Cells. 2020 Jan 21;9(2). pii: cells9020262. doi: 10.3390/cells9020262. PubMed |
| Chromosome 19 microRNA cluster enhances cell reprogramming by inhibiting epithelial-to-mesenchymal transition. Mong EF, Yang Y, Akat KM, Canfield J, VanWye J, Lockhart J, Tsibris JCM, Schatz F, Lockwood CJ, Tuschl T, Kayisli UA, Totary-Jain H. Sci Rep. 2020 Feb 20;10(1):3029. doi: 10.1038/s41598-020-59812-8. PubMed |
| Prioritizing disease and trait causal variants at the TNFAIP3 locus using functional and genomic features. Ray JP, de Boer CG, Fulco CP, Lareau CA, Kanai M, Ulirsch JC, Tewhey R, Ludwig LS, Reilly SK, Bergman DT, Engreitz JM, Issner R, Finucane HK, Lander ES, Regev A, Hacohen N. Nat Commun. 2020 Mar 6;11(1):1237. doi: 10.1038/s41467-020-15022-4. PubMed |
| A distal enhancer at risk locus 11q13.5 promotes suppression of colitis by Treg cells. Nasrallah R, Imianowski CJ, Bossini-Castillo L, Grant FM, Dogan M, Placek L, Kozhaya L, Kuo P, Sadiyah F, Whiteside SK, Mumbach MR, Glinos D, Vardaka P, Whyte CE, Lozano T, Fujita T, Fujii H, Liston A, Andrews S, Cozzani A, Yang J, Mitra S, Lugli E, Chang HY, Unutmaz D, Trynka G, Roychoudhuri R. Nature. 2020 Jul;583(7816):447-452. doi: 10.1038/s41586-020-2296-7. Epub 2020 May 13. PubMed |
Enhancer RNAs predict enhancer-gene regulatory links and are critical for enhancer function in neuronal systems.
Carullo NVN, Phillips Iii RA, Simon RC, Soto SAR, Hinds JE, Salisbury AJ, Revanna JS, Bunner KD, Ianov L, Sultan FA, Savell KE, Gersbach CA, Day JJ.
Nucleic Acids Res. 2020 Aug 18. pii: 5893972. doi: 10.1093/nar/gkaa671.
PubMed
Associated Plasmids |
| Controlling the Activity of CRISPR Transcriptional Regulators with Inducible sgRNAs. Ferry QRV, Fulga TA. Methods Mol Biol. 2021;2162:153-184. doi: 10.1007/978-1-0716-0687-2_9. PubMed |
| Identification of human long noncoding RNAs associated with nonalcoholic fatty liver disease and metabolic homeostasis. Ruan X, Li P, Ma Y, Jiang CF, Chen Y, Shi Y, Gupta N, Seifuddin F, Pirooznia M, Ohnishi Y, Yoneda N, Nishiwaki M, Dumbovic G, Rinn JL, Higuchi Y, Kawai K, Suemizu H, Cao H. J Clin Invest. 2021 Jan 4;131(1). pii: 136336. doi: 10.1172/JCI136336. PubMed |
| Identification and characterization of a new isoform of small GTPase RhoE. Dai Y, Luo W, Yue X, Ma W, Wang J, Chang J. Commun Biol. 2020 Oct 15;3(1):572. doi: 10.1038/s42003-020-01295-4. PubMed |
| Transcriptional behavior of the HIV-1 promoter in context of the BACH2 prominent proviral integration gene. Hamann MV, Ehmele P, Verdikt R, Bialek-Waldmann JK, Virdi S, Gunther T, Van Lint C, Grundhoff A, Hauber J, Lange UC. Virus Res. 2021 Feb;293:198260. doi: 10.1016/j.virusres.2020.198260. Epub 2020 Dec 11. PubMed |
| HIV-based lentiviral vectors: origin and sequence differences. Johnson NM, Alvarado AF, Moffatt TN, Edavettal JM, Swaminathan TA, Braun SE. Mol Ther Methods Clin Dev. 2021 Mar 27;21:451-465. doi: 10.1016/j.omtm.2021.03.018. eCollection 2021 Jun 11. PubMed |
| Titan Cells and Yeast Forms of Cryptococcus neoformans and Cryptococcus gattii Are Recognized by GXMR-CAR. Dos Santos MH, Machado MP, Kumaresan PR, da Silva TA. Microorganisms. 2021 Sep 5;9(9). pii: microorganisms9091886. doi: 10.3390/microorganisms9091886. PubMed |
| Salicylic acid inducible nucleocytoplasmic shuttling of NPR1 fusion proteins in human cells. Sadeghi F, Kumar M, Bandey IN, Li X, Roysam B, Varadarajan N. Biotechnol Bioeng. 2022 Jan;119(1):199-210. doi: 10.1002/bit.27966. Epub 2021 Nov 3. PubMed |
| Genome-wide CRISPR interference screen identifies long non-coding RNA loci required for differentiation and pluripotency. Haswell JR, Mattioli K, Gerhardinger C, Maass PG, Foster DJ, Peinado P, Wang X, Medina PP, Rinn JL, Slack FJ. PLoS One. 2021 Nov 3;16(11):e0252848. doi: 10.1371/journal.pone.0252848. eCollection 2021. PubMed |
| Homeoprotein SIX1 compromises antitumor immunity through TGF-beta-mediated regulation of collagens. Liu W, Gao M, Li L, Chen Y, Fan H, Cai Q, Shi Y, Pan C, Liu J, Cheng LS, Yang H, Cheng G. Cell Mol Immunol. 2021 Dec;18(12):2660-2672. doi: 10.1038/s41423-021-00800-x. Epub 2021 Nov 15. PubMed |
| Histone marks regulate the epithelial-to-mesenchymal transition via alternative splicing. Segelle A, Nunez-Alvarez Y, Oldfield AJ, Webb KM, Voigt P, Luco RF. Cell Rep. 2022 Feb 15;38(7):110357. doi: 10.1016/j.celrep.2022.110357. PubMed |
| CDK2 Inhibition Enhances Antitumor Immunity by Increasing IFN Response to Endogenous Retroviruses. Chen Y, Cai Q, Pan C, Liu W, Li L, Liu J, Gao M, Li X, Wang L, Rao Y, Yang H, Cheng G. Cancer Immunol Res. 2022 Apr 1;10(4):525-539. doi: 10.1158/2326-6066.CIR-21-0806. PubMed |
The nuclear receptor THRB facilitates differentiation of human PSCs into more mature hepatocytes.
Ma H, de Zwaan E, Guo YE, Cejas P, Thiru P, van de Bunt M, Jeppesen JF, Syamala S, Dall'Agnese A, Abraham BJ, Fu D, Garrett-Engele C, Lee TI, Long HW, Griffith LG, Young RA, Jaenisch R.
Cell Stem Cell. 2022 Apr 13. pii: S1934-5909(22)00150-3. doi: 10.1016/j.stem.2022.03.015.
PubMed
Associated Plasmids |
| Endothelial plasticity drives aberrant vascularization and impedes cardiac repair after myocardial infarction. Huang M, Yang F, Zhang D, Lin M, Duan H, El-Mayta R, Zhang L, Qin L, Shewale SV, Pei L, Mitchell MJ, Rader DJ, Fan Y, Gong Y. Nat Cardiovasc Res. 2022 Apr;1(4):372-388. doi: 10.1038/s44161-022-00047-3. Epub 2022 Apr 13. PubMed |
| Small-Molecule-Mediated Split-Aptamer Assembly for Inducible CRISPR-dCas9 Transcription Activation. Liu XH, Li BR, Ying ZM, Tang LJ, Wang F, Jiang JH. ACS Chem Biol. 2022 Jul 15;17(7):1769-1777. doi: 10.1021/acschembio.2c00101. Epub 2022 Jun 14. PubMed |
| CRISPR activation screen identifies TGFbeta-associated PEG10 as a crucial tumor suppressor in Ewing sarcoma. Saratov V, Ngo QA, Pedot G, Sidorov S, Wachtel M, Niggli FK, Schafer BW. Sci Rep. 2022 Jun 23;12(1):10671. doi: 10.1038/s41598-022-12659-7. PubMed |
| PAM-flexible dual base editor-mediated random mutagenesis and self-activation strategies to improve CRISPRa potency. Lau CH, Huang S, Lam RHW, Tin C. Mol Ther Methods Clin Dev. 2022 May 29;26:26-37. doi: 10.1016/j.omtm.2022.05.005. eCollection 2022 Sep 8. PubMed |
| YAP1 induces invadopodia formation by transcriptionally activating TIAM1 through enhancer in breast cancer. Shen J, Huang Q, Jia W, Feng S, Liu L, Li X, Tao D, Xie D. Oncogene. 2022 Jul;41(31):3830-3845. doi: 10.1038/s41388-022-02344-4. Epub 2022 Jun 30. PubMed |
| A Ctnnb1 enhancer regulates neocortical neurogenesis by controlling the abundance of intermediate progenitors. Wang J, Wang A, Tian K, Hua X, Zhang B, Zheng Y, Kong X, Li W, Xu L, Wang J, Li Z, Liu Y, Zhou Y. Cell Discov. 2022 Aug 2;8(1):74. doi: 10.1038/s41421-022-00421-2. PubMed |
| Arc Regulates Transcription of Genes for Plasticity, Excitability and Alzheimer's Disease. Leung HW, Foo G, VanDongen A. Biomedicines. 2022 Aug 11;10(8):1946. doi: 10.3390/biomedicines10081946. PubMed |
| CRISPR-mediated activation of autism gene Itgb3 restores cortical network excitability via mGluR5 signaling. Jaudon F, Thalhammer A, Zentilin L, Cingolani LA. Mol Ther Nucleic Acids. 2022 Jul 20;29:462-480. doi: 10.1016/j.omtn.2022.07.013. eCollection 2022 Sep 13. PubMed |
Activation of stably silenced genes by recruitment of a synthetic de-methylating module.
Chan WF, Coughlan HD, Chen Y, Keenan CR, Smyth GK, Perkins AC, Johanson TM, Allan RS.
Nat Commun. 2022 Sep 23;13(1):5582. doi: 10.1038/s41467-022-33181-4.
PubMed
Associated Plasmids |
| A lncRNA identifies Irf8 enhancer element in negative feedback control of dendritic cell differentiation. Xu H, Li Z, Kuo CC, Gotz K, Look T, de Toledo MAS, Sere K, Costa IG, Zenke M. Elife. 2023 Mar 14;12:e83342. doi: 10.7554/eLife.83342. PubMed |
| A novel lentiviral vector-based approach to generate chimeric antigen receptor T cells targeting Aspergillus fumigatus. Kumaresan PR, Wurster S, Bavisi K, da Silva TA, Hauser P, Kinnitt J, Albert ND, Bharadwaj U, Neelapu S, Kontoyiannis DP. mBio. 2024 Apr 10;15(4):e0341323. doi: 10.1128/mbio.03413-23. Epub 2024 Feb 28. PubMed |
| The sirtuin-associated human senescence program converges on the activation of placenta-specific gene PAPPA. Bi S, Jiang X, Ji Q, Wang Z, Ren J, Wang S, Yu Y, Wang R, Liu Z, Liu J, Hu J, Sun G, Wu Z, Diao Z, Li J, Sun L, Izpisua Belmonte JC, Zhang W, Liu GH, Qu J. Dev Cell. 2024 Apr 22;59(8):991-1009.e12. doi: 10.1016/j.devcel.2024.02.008. Epub 2024 Mar 13. PubMed |
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