pBS-Isce1-eno2:egfp
(Plasmid
#61394)
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PurposeExpresses GFP in neurons of transgenic zebrafish
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61394 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS
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Modifications to backboneI-sce1 sites
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Vector typeZebrafish expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeno2 regulatory element
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)12000
- Promoter eno2
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-Isce1-eno2:egfp was a gift from Edward Burton (Addgene plasmid # 61394 ; http://n2t.net/addgene:61394 ; RRID:Addgene_61394) -
For your References section:
Generation of a transgenic zebrafish model of Tauopathy using a novel promoter element derived from the zebrafish eno2 gene. Bai Q, Garver JA, Hukriede NA, Burton EA. Nucleic Acids Res. 2007;35(19):6501-16. Epub 2007 Sep 25. 10.1093/nar/gkm608 PubMed 17897967
Map uploaded by the depositor.
