pBS-KS-attB1-2-PT-SA-SD-2-2xTY1-V5
(Plasmid
#61257)
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PurposeCRISPR-RMCE, second step attB plasmid, intron phase 2, exchange dsRed with 2xTY1 and V5 tags cassette
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61257 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS-KS-attB1-2-PT-SA-SD
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Backbone manufacturerHugo J Bellen
- Backbone size w/o insert (bp) 3300
- Total vector size (bp) 3541
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name2xTY1-V5
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SpeciesSynthetic
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Insert Size (bp)216
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Tags
/ Fusion Proteins
- TY1
- V5
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-KS-attB1-2-PT-SA-SD-2-2xTY1-V5 was a gift from Frank Schnorrer (Addgene plasmid # 61257 ; http://n2t.net/addgene:61257 ; RRID:Addgene_61257) -
For your References section:
A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila. Zhang X, Koolhaas WH, Schnorrer F. G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 10.1534/g3.114.013979. 10.1534/g3.114.013979 PubMed 25324299
