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PurposeLentiviral DOX-inducible expression vector for human NANOG
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 60849 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUW-tetO-loxP
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehNANOG
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SpeciesH. sapiens (human)
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Insert Size (bp)918
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Entrez GeneNANOG
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer LNCX
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FUW-tetO-loxP-hNANOG was a gift from Rudolf Jaenisch (Addgene plasmid # 60849 ; http://n2t.net/addgene:60849 ; RRID:Addgene_60849) -
For your References section:
Systematic identification of culture conditions for induction and maintenance of naive human pluripotency. Theunissen TW, Powell BE, Wang H, Mitalipova M, Faddah DA, Reddy J, Fan ZP, Maetzel D, Ganz K, Shi L, Lungjangwa T, Imsoonthornruksa S, Stelzer Y, Rangarajan S, D'Alessio A, Zhang J, Gao Q, Dawlaty MM, Young RA, Gray NS, Jaenisch R. Cell Stem Cell. 2014 Oct 2;15(4):471-87. doi: 10.1016/j.stem.2014.07.002. Epub 2014 Jul 24. 10.1016/j.stem.2014.07.002 PubMed 25090446