bfloGFPc1a
(Plasmid
#60750)
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PurposeBacterial expression of Amphioxus GFPc1
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60750 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHIS8
- Backbone size w/o insert (bp) 5260
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsProtein can be expressed in BL21 codon+ cells. It should be grown at 37C for 4 hours followed by 20C for 6 hours.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGFPc1
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SpeciesBranchiostoma floridae (amphioxus)
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Insert Size (bp)678
- Promoter T7
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Tag
/ Fusion Protein
- 8xHIS (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-Term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
bfloGFPc1a was a gift from Dimitri Deheyn (Addgene plasmid # 60750 ; http://n2t.net/addgene:60750 ; RRID:Addgene_60750) -
For your References section:
Spectral and structural comparison between bright and dim green fluorescent proteins in Amphioxus. Bomati EK, Haley JE, Noel JP, Deheyn DD. Sci Rep. 2014 Jun 27;4:5469. doi: 10.1038/srep05469. 10.1038/srep05469 PubMed 24968921