PuroR-P2A-DD-TCOF1-donor
(Plasmid #60747)

• Purpose: Donor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1
• Depositing Lab: Benhur Lee
• Publication: Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi: 10.1371/journal.pone.0095101. eCollection 2014.

Backbone

• Vector backbone: PCR2.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3900
• Total vector size (bp): 6500
• Vector type: Targeting donor
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TCOF1
• Alt name: Treacle
• Species: H. sapiens (human)
• Insert Size (bp): 2588
• Entrez Gene: TCOF1 (a.k.a. MFD1, TCS, TCS1, treacle)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: M13R
• 3′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • Annotated sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry