pMSCV-Pidd1(T788A)
(Plasmid #60529)

• Purpose: Retroviral expression of Pidd1 T788A phosphorylation mutant
• Depositing Lab: Trudy Oliver
• Publication: Terry et al Cell Death Differ. 2014 Oct 10. doi: 10.1038/cdd.2014.159.

Backbone

• Vector backbone: pMSCV
• Backbone size w/o insert (bp): 7000
• Total vector size (bp): 10000
• Modifications to backbone: JL090 MCS (HindIII-ApaI) cloned into pMSCV vector using HindIII and ApaI
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Pidd1
• Alt name: Lrdd1
• Species: H. sapiens (human)
• Insert Size (bp): 2800
• Mutation: Threonine 788 to Alanine*
• GenBank ID: NM_145886
• Entrez Gene: PIDD1 (a.k.a. LRDD, PIDD)
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: TGACACGGTGGAGATGTTC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Antoine Tinel in Jurg Tschopp's lab cloned pMSCV-Pidd1. We generated point mutation using site-directed mutagenesis. *The Tschopp clone also contains V806D and R877H mutations, which are not known to affect function.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry