pmTurquoise2-T2A-Venus(L68V)
(Plasmid #60494)

• Purpose: Produces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.
• Depositing Lab: Dorus Gadella
• Publication: Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1038/ncomms1738.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5441
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mTurquoise2-T2A-mVenus(L68V)
• Alt name: mTurquoise2 & SYFP2
• Species: Synthetic
• Insert Size (bp): 1497
• Promoter: CMV
• Tag / Fusion Protein:
  • T2A peptide

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BsrGI (not destroyed)
• 5′ sequencing primer: CMV

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry