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pCDH-CMV-MSI1(FL)
(Plasmid #60358)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60358 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH-CMV-MCS-EF1
  • Backbone manufacturer
    System Biosciences
  • Backbone size w/o insert (bp) 8300
  • Total vector size (bp) 9300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse MSI1 (FL)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1089
  • GenBank ID
    NM_008629.1
  • Entrez Gene
    Msi1 (a.k.a. Msi1h, Musahi1, m-Msi-1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-CMV-MSI1(FL) was a gift from Sean Ryder (Addgene plasmid # 60358 ; http://n2t.net/addgene:60358 ; RRID:Addgene_60358)

  • For your References section:

    Allosteric inhibition of a stem cell RNA-binding protein by an intermediary metabolite. Clingman CC, Deveau LM, Hay SA, Genga RM, Shandilya SM, Massi F, Ryder SP. Elife. 2014 Jun 16:e02848. doi: 10.7554/eLife.02848. 10.7554/eLife.02848 PubMed 24935936
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