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Addgene

pSP72 GSK3beta
(Plasmid #60125)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60125 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP72
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2400
  • Total vector size (bp) 5100
  • Vector type
    cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Glycogen synthase kinase 3 beta
  • Alt name
    GSK3beta
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2700
  • Entrez Gene
    Gsk3b (a.k.a. 7330414F15Rik, 8430431H08Rik, GSK-3, GSK-3beta, GSK3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We received this from Dr. Andrew McMahon at Harvard University in 1996. It was cloned by Scott Lee in his lab.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSP72 GSK3beta was a gift from Andrew McMahon (Addgene plasmid # 60125 ; http://n2t.net/addgene:60125 ; RRID:Addgene_60125)
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