pcDNA3.1/hpa5(aIIB_thigh)
(Plasmid #59971)

• Purpose: integrin a5 chimeric headpiece expression (a5 beta-propeller domain and aIIB thigh domain)
• Depositing Lab: Timothy Springer
• Publication: integrin a5b1 headpiece (unpublished)

Backbone

• Vector backbone: pcDNA3.1/Hygro(-)
• Backbone size w/o insert (bp): 5596
• Total vector size (bp): 7602
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human integrin a5 beta-propeller domain fused with human integrin aIIB thigh domain
• Species: H. sapiens (human)
• Insert Size (bp): 2076
• Entrez Gene: ITGA5 (a.k.a. CD49e, FNRA, VLA-5, VLA5A)
• Entrez Gene: ITGA2B (a.k.a. BDPLT16, BDPLT2, CD41, CD41B, GP2B, GPIIb, GT, GT1, GTA, HPA3, PPP1R93)
• Promoter: CMV
• Tag / Fusion Protein:
  • Acid coiled-coil and StrepII tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CMV_fwd
• 3′ sequencing primer: BGH_rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry