pSAG1::CAS9-U6::sg290860-6
(Plasmid
#59855)
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PurposeEncodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 59855 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBlueScript
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Backbone manufacturerAgilent
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Vector typeCRISPR ; ; Toxoplasma gondii
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCRISPR sg290860-6
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSAG1::CAS9-U6::sg290860-6 was a gift from David Sibley (Addgene plasmid # 59855 ; http://n2t.net/addgene:59855 ; RRID:Addgene_59855) -
For your References section:
Genetic mapping reveals that sinefungin resistance in Toxoplasma gondii is controlled by a putative amino acid transporter locus that can be used as a negative selectable marker. Behnke MS, Khan A, Sibley LD. Eukaryot Cell. 2014 Dec 5. pii: EC.00229-14. 10.1128/EC.00229-14 PubMed 25480939
Map uploaded by the depositor.
