pSA108
(Plasmid #59786)

• Purpose: drives mCherry in C. elegans ubiquitously
• Depositing Lab: Jeremy Nance
• Publication: Armenti et al Development. 2014 Dec;141(23):4640-7. doi: 10.1242/dev.115048. Epub 2014 Nov 5.

Backbone

• Vector backbone: pCFJ150
• Modifications to backbone: modified to include PmeI restriction site for Gibson cloning
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: cdc-42 promoter
• Species: C. elegans (nematode)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ctgtcagaccaagtttactcatatatactttagattg
• 3′ sequencing primer: TTCGCCTGAAAAAAAAAATGAATAAAAGTATTAAAATTAC

Gene/Insert 2

• Gene/Insert name: mCherry (worm)
• Species: C. elegans (nematode)

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ATGGTCTCAAAGGGTGAAGAAG
• 3′ sequencing primer: CTACTTATACAATTCATCCATGCCAC

Gene/Insert 3

• Gene/Insert name: unc-54 3' UTR

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gtccaattactcttcaacatccc
• 3′ sequencing primer: AAACAGTTATGTTTGGTATATTGGGAATG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry