pNSEN-d2
(Plasmid #59763)

• Purpose: Expresses nuclear EGFP and Tomato in mammalian cells
• Depositing Lab: Jerry Crabtree
• Publication: Yoo et al Nature. 2009 Jul 30;460(7255):642-6. doi: 10.1038/nature08139. Epub 2009 Jun 28.

Backbone

• Vector backbone: pUC
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: d2-EGFP
• Alt name: destablized EGFP
• Insert Size (bp): 935
• Promoter: SV40 promoter
• Tags / Fusion Proteins:
  • 3X NLS (N terminal on insert)
  • PEST (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: GACGAAAGATTGGTGTGGAAAGTCC
• 3′ sequencing primer: atcatcctgctcctccacctcc

Gene/Insert 2

• Gene/Insert name: Tomato
• Alt name: monomeric Tomato
• Insert Size (bp): 785
• Promoter: CMV promoter
• Tag / Fusion Protein:
  • 3X NLS (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: CACTGCAATCTCGTGATACGCCTA
• 3′ sequencing primer: tgatgagtttggacaaaccacaac

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry