HC080
(Plasmid
#59545)
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PurposeExpresses yeast Atg13 under the endogenous promoter with a GFP N-terminal tag
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 59545 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS315
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameATG13
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Alt nameAPG13
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SpeciesS. cerevisiae (budding yeast)
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MutationAvrII site created after start codon
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GenBank IDNM_001184282.1
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Entrez GeneATG13 (a.k.a. YPR185W, APG13)
- Promoter ATG13
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Tag
/ Fusion Protein
- GFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer M13-R
- 3′ sequencing primer M13-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe original ATG13 plasmid was from Yoshinori Ohsumi.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HC080 was a gift from Daniel Klionsky (Addgene plasmid # 59545 ; http://n2t.net/addgene:59545 ; RRID:Addgene_59545)