pPOP
(Plasmid
#59526)
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Purpose(Empty Backbone) Vector for pop-in/pop-out gene replacement in Pichia pastoris.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 59526 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPOP
- Backbone size (bp) 5538
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Vector typeYeast Expression
- Promoter GAP
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Selectable markersARG4 and T-urf13
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer Lac_promoter (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPOP was a gift from Benjamin Glick (Addgene plasmid # 59526 ; http://n2t.net/addgene:59526 ; RRID:Addgene_59526) -
For your References section:
Vector for pop-in/pop-out gene replacement in Pichia pastoris. Soderholm J, Bevis BJ, Glick BS. Biotechniques. 2001 Aug;31(2):306-10, 312. 10.2144/01312st01 PubMed 11515366
Map uploaded by the depositor.
