pAAV-synP-FLEX-EGFP-B19G
(Plasmid
#59333)
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PurposeExpresses EGFP-P2A-B19G
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 59333 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4437
- Total vector size (bp) 6795
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Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsStbl3 at 37C
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP-P2A-B19G
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SpeciesSynthetic
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Insert Size (bp)2358
- Promoter Synapsin-1
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer AGGCGCGAGATAGGg
- 3′ sequencing primer cagcgtatccacatagcg (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Alternative name: pAAV-synP-FLEX-EpB
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-synP-FLEX-EGFP-B19G was a gift from Ian Wickersham (Addgene plasmid # 59333 ; http://n2t.net/addgene:59333 ; RRID:Addgene_59333)