pEGFP-C1-alpha1-chimaerin
(Plasmid #59317)

• Purpose: Expression plasmid for GFP-tagged alpha1-chimaerin (GFP at N-terminus of alpha1-chimaerin)
• Depositing Lab: Peter Scheiffele
• Publication: Beg et al Neuron. 2007 Sep 6;55(5):768-78.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 4723
• Total vector size (bp): 5728
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: alpha1-chimaerin
• Insert Size (bp): 1005
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV Forward (5'-CGCAAATGGGCGGTAGGCGTG-3'); EGFP-C (5'-CATGGTCCTGCTGGAGTTCGTG-3')
• 3′ sequencing primer: pEGFP_rev_seq (5'-AAGCAAGTAAAACCTCTACAAATG-3'); SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')

Resource Information

• Supplemental Documents:
  • Sequence file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See map & sequence file for insert-releasing enzymes.
alpha1-chimaerin can be released without GFP-tag (e.g. by SalI / BclI) or with GFP-tag (e.g. by AgeI / BclI).
Note: BclI is blocked by dam methylation.