Gfap-Cryab
(Plasmid #58947)

• Purpose: hamster Cryab gene under the control of the human GFAP promoter to target expression to astrocytes
• Depositing Lab: Albee Messing
• Publication: Hagemann et al Hum Mol Genet. 2009 Apr 1;18(7):1190-9. doi: 10.1093/hmg/ddp013. Epub 2009 Jan 7.

Backbone

• Vector backbone: pBluescript(SK)
• Backbone manufacturer: Stratagene
• Vector type: Mammalian Expression, Mouse Targeting ; transgene expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cryab
• Alt name: crystallin, alpha B
• Species: hamster
• Insert Size (bp): 6000
• Mutation: genomic fragment
• Promoter: human GFAP promoter (position -2163 to +47)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gfap- Michael Brenner, Professor, Dept. of Neurobiology, University of Alabama-Birmingham Cryab - Wilbert C. Boelens, Dept. of Biomolecular Chemistry, Radbound University Nijmegen, Netherlands

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The transgene includes the translation initiation site with 90 bp of 5'UTR, polyadenylation signal and all three exons of Cryab. Restriction sites within the cloning plasmid linker sequences were used to ligate the GFAP and Cryab fragments together.
NotI was used to excise the transgene (Tg141) from the construct vector.