pET22b RNase A
(Plasmid #58903)

• Purpose: Bacterial expression of bovine pancreatic ribonuclease A
• Depositing Lab: Ronald Raines
• Publication: delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

Backbone

• Vector backbone: pET22b(+)
• Backbone manufacturer: EMD Millipore
• Backbone size w/o insert (bp): 5493
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RNase A
• Species: B. taurus (bovine)
• Insert Size (bp): 500
• Mutation: See Depositor Comments below
• GenBank ID: NP_001014408
• Entrez Gene: RNASE1 (a.k.a. RNS1)
• Promoter: T7
• Tag / Fusion Protein:
  • pelB sequence (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (destroyed during cloning)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-Term

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the pelB sequence is maintained however there are stop codons incorporated at the end of the gene so the His-Tag is not in frame. The pelB is clipped off and the gene encodes 124 amino acid protein that is expressed/purified from inclusion bodies.