Skip to main content
Addgene

p5'Entry-lck-7.4kb
(Plasmid #58891)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58891 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pDONR P4-P1R
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4777
  • Total vector size (bp) 10030
  • Vector type
    Gateway 5' entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 7.4 kb
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    7387
  • Mutation
    mutated single nucleotide (T-->C, 1874th nucleotide of insert) to create novel SfiI site
  • Promoter lymphocyte-specific protein tyrosine kinase (lck), 7.4 kb

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5'Entry-lck-7.4kb was a gift from Jeffrey Yoder (Addgene plasmid # 58891 ; http://n2t.net/addgene:58891 ; RRID:Addgene_58891)
Related items:
Commonly requested with: