pAAV-Syn-ChR2-GFP
(Plasmid #58881)

• Purpose: AAV expression of ChR2-GFP under the Syn promoter
• Depositing Lab: Edward Boyden
• Publication: Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Backbone

• Vector backbone: AAV
• Backbone size w/o insert (bp): 4697
• Total vector size (bp): 6362
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Stbl3 at 30C (use carbenicillin if using Stbl3) OR DH5a at 37C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ChR2-GFP
• Alt name: channelrhodopsin-2
• Species: Chlamydomonas reinhardtii
• Insert Size (bp): 1665
• GenBank ID: EU714030.1
• Promoter: Syn
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CTGCGTATGAGTGCAAG
• 3′ sequencing primer: cagcgtatccacatagcg

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid is completely sequenced by the depositing lab except for parts of the origin and 3' ITR. Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.