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PurposeAAV-mediated expression of ChR2-mCherry under the GFAP promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 58892 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV
- Backbone size w/o insert (bp) 4895
- Total vector size (bp) 6545
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsStbl3 at 30C (use carbenicillin if using Stbl3) OR DH5a at 37C
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameChR2-mCherry
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Alt namechannelrhodopsin-2-mCherry
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SpeciesChlamydomonas reinhardtii
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Insert Size (bp)1650
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GenBank IDEU714030.1
- Promoter GFAP104
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Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer ggagagctctccccatag
- 3′ sequencing primer cagcgtatccacatagcg (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The depositing lab has sequenced the 5' ITR, promoter, and gene-fluorophore. Multiple digestions were done to verify the vector structure. The construct was tested in vitro and the virus was tested both in vitro and in vivo.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-GFAP104-ChR2-mCherry was a gift from Edward Boyden (Addgene plasmid # 58892 ; http://n2t.net/addgene:58892 ; RRID:Addgene_58892) -
For your References section:
Optogenetic astrocyte activation modulates response selectivity of visual cortex neurons in vivo. Perea G, Yang A, Boyden ES, Sur M. Nat Commun. 2014;5:3262. doi: 10.1038/ncomms4262. 10.1038/ncomms4262 PubMed 24500276