pRS406-PHO5-GFP-Scs2
(Plasmid
#58834)
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PurposeExpresses GFP-Tagged Scs2 in yeast
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58834 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS406
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Backbone manufacturerStratagene
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameScs2
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Alt nameSCS2p
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Alt nameYER120W
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SpeciesS. cerevisiae (budding yeast)
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GenBank IDNM_001179010.3
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Entrez GeneSCS2 (a.k.a. YER120W)
- Promoter PHO5
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Tags
/ Fusion Proteins
- GFP (N terminal on backbone)
- Myc (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS406-PHO5-GFP-Scs2 was a gift from Tim Levine (Addgene plasmid # 58834 ; http://n2t.net/addgene:58834 ; RRID:Addgene_58834) -
For your References section:
A conserved ER targeting motif in three families of lipid binding proteins and in Opi1p binds VAP. Loewen CJ, Roy A, Levine TP. EMBO J. 2003 May 1;22(9):2025-35. 10.1093/emboj/cdg201 PubMed 12727870