pRS406-PHO5-GFP-CERT PH domain EE mutation
(Plasmid #58833)

• Purpose: Expresses GFP-Tagged and mutated CERT PH domain in yeast
• Depositing Lab: Tim Levine
• Publication: Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9. Epub 2004 Jul 22.

Backbone

• Vector backbone: pRS406
• Backbone manufacturer: Stratagene
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: collagen, type IV, alpha 3 binding protein PH domain
• Alt name: CERT
• Alt name: GPBP
• Species: H. sapiens (human)
• Mutation: changed Arginine 43 to Glutamate
• GenBank ID: NM_001130105.1
• Entrez Gene: CERT1 (a.k.a. CERT, CERTL, COL4A3BP, GPBP, MRD34, STARD11)
• Promoter: PHO5
• Tags / Fusion Proteins:
  • GFP (N terminal on backbone)
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PH domain of CERT= Methionine 1 - Glycine 122