pAAV-CAG-FLEX-Mac-GFP
(Plasmid #58803)

• Purpose: AAV-mediated expression of Mac-GFP under the CAG promoter, in floxed/reversed (Cre-dependent) manner
• Depositing Lab: Edward Boyden
• Publication: Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Backbone

• Vector backbone: AAV-FLEX
• Backbone manufacturer: Scott Sternson
• Backbone size w/o insert (bp): 5036
• Total vector size (bp): 6707
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Stbl3 at 30C (use carbenicillin if using Stbl3) OR DH5a at 37C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Mac-GFP
• Alt name: Leptosphaeria maculans opsin
• Species: Leptosphaeria maculans
• Insert Size (bp): 1671
• GenBank ID: GU045595.1
• Promoter: CAG
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BsrGI (not destroyed)
• 5′ sequencing primer: tgctaaccatgttcatgc
• 3′ sequencing primer: cagcgtatccacatagcg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid is completely sequenced by depositing lab except for parts of both ITRs and a part of the CAG promoter. Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.