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Addgene

p(+)MV-NSE-FlagrP-3g_haP-F497D-3p Notes

Plasmid 58800 digest

These results confirm the presence of the unique AatII site noted as a backbone modification. The plasmid was digested with NotI and AatII as single and double digests. Linearized plasmid is 20.8 kb. NotI/AatII fragments are 8.1kb & 12.75kb.