pTetO-ISce1-TetO
(Plasmid #58506)

• Purpose: ISce1 restriction site is flanked by Tet operator repeats, 96 on each side
• Depositing Lab: Tom Misteli
• Publication: Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi: 10.1126/science.1237150.

Backbone

• Vector backbone: pBluescript II KS
• Backbone size w/o insert (bp): 3500
• Total vector size (bp): 8000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Growth instructions: Plasmids are transformed in Stbl2 competent cells (Invitrogen). Grow always in 30oC to avoid recombination events and check the digestion pattern (e.g. cut with XhoI-SacII). Check this every time you propagate these plasmids, since recombination events are very often.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Tet repeats flanking ISce1 restriction site
• Species: bacterial Tet operator
• Insert Size (bp): 8000
• Promoter: no promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: M13 pUC forward primer
• 3′ sequencing primer: M13pUC reverse primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that though EcoRI is listed within the MCS of the depositor's map, it is NOT unique within this plasmid. Please do not use it for cloning purposes.