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Addgene

pRL528
(Plasmid #58495)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58495 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRL542

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    HB101
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    encodes methylases AvaiM and Eco47iiM that target sites subject to restriction in Anabaena sp. strain PCC 7120
  • Alt name
    MobColK, M.AvaI, M.Eco47II

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MobColK, M.AvaI, M.Eco47II

Please see attached map for more information on how this vector was constructed and cloning details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRL528 was a gift from Peter Wolk (Addgene plasmid # 58495 ; http://n2t.net/addgene:58495 ; RRID:Addgene_58495)

  • For your References section:

    Conjugal transfer of DNA to cyanobacteria. Elhai J, Wolk CP. Methods Enzymol. 1988;167:747-54. 0076-6879(88)67086-8 [pii] PubMed 3148842
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