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Addgene

NPC1-CFP P692S
(Plasmid #58457)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58457 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECFP-N3
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NPC1
  • Species
    M. musculus (mouse)
  • Mutation
    P692S sterol sensing domain mutant
  • Entrez Gene
    Npc1 (a.k.a. A430089E03Rik, D18Ertd139e, D18Ertd723e, lcsd, nmf164, spm)
  • Promoter CMV
  • Tags / Fusion Proteins
    • His6 (C terminal on insert)
    • ECFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NPC1-CFP P692S was a gift from Matthew Scott (Addgene plasmid # 58457 ; http://n2t.net/addgene:58457 ; RRID:Addgene_58457)

  • For your References section:

    Dynamic movements of organelles containing Niemann-Pick C1 protein: NPC1 involvement in late endocytic events. Ko DC, Gordon MD, Jin JY, Scott MP. Mol Biol Cell. 2001 Mar;12(3):601-14. 10.1091/mbc.12.3.601 PubMed 11251074
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