mTagRFP-T2-PCNA-19
(Plasmid #58043)

• Purpose: Localization: Nucleus, Excitation: 555, Emission: 584
• Depositing Lab: Michael Davidson
• Publication: Davidson Orange Fluorescent Proteins (unpublished)

Backbone

• Vector backbone: mTagRFP
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PCNA
• Alt name: Human Proliferating Cell Nuclear Antigen + SV40 NLS 3x Repeat
• Alt name: NM_002592.2
• Species: H. sapiens (human)
• Entrez Gene: PCNA (a.k.a. ATLD2)
• Promoter: CMV
• Tag / Fusion Protein:
  • mTagRFP

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Industry MTA
  • Evrogen Ancillary Agreement for FP Distribution to Industry

Depositor Comments

H. sapiens (human) Proliferating Cell Nuclear Antigen + SV40 NLS 3x Repeat. Excitation = 555; Emission = 584