mKO-EB3-7
(Plasmid
#57838)
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PurposeLocalization: MT End Binding Protein, Excitation: 548, Emission: 561
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 57838 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonemKO
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEB3
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Alt nameNM_012326.2
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SpeciesH. sapiens (human)
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Entrez GeneMAPRE3 (a.k.a. EB3, EBF3, EBF3-S, RP3)
- Promoter CMV
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Tag
/ Fusion Protein
- mKO
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 548; Emission = 561
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mKO-EB3-7 was a gift from Michael Davidson (Addgene plasmid # 57838 ; http://n2t.net/addgene:57838 ; RRID:Addgene_57838) -
For your References section:
Automated screening of microtubule growth dynamics identifies MARK2 as a regulator of leading edge microtubules downstream of Rac1 in migrating cells. Nishimura Y, Applegate K, Davidson MW, Danuser G, Waterman CM. PLoS One. 2012;7(7):e41413. doi: 10.1371/journal.pone.0041413. Epub 2012 Jul 24. 10.1371/journal.pone.0041413 PubMed 22848487