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Addgene

mCherry-UtrCH
(Plasmid #26740)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26740 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4097
  • Vector type
    Mammalian Expression ; reporter for F-actin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Utrophin
  • Alt name
    UtrCH
  • Species
    unknown
  • Mutation
    Calponin homology domain of utrophin (aa# 1-261)
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The calponin homology domain of utrophin (Utr-CH) was fused to mCherry in order to visualize F-actin.

mCherry R154 mutated (CGG to AGA) for optimal codon usage in Xenopus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-UtrCH was a gift from William Bement (Addgene plasmid # 26740 ; http://n2t.net/addgene:26740 ; RRID:Addgene_26740)
  • For your References section:

    Versatile fluorescent probes for actin filaments based on the actin-binding domain of utrophin. Burkel BM, von Dassow G, Bement WM. Cell Motil Cytoskeleton. 2007 Nov . 64(11):822-32. 10.1002/cm.20226 PubMed 17685442