mEos2-Hec1-C-12
(Plasmid
#57386)
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PurposeLocalization: Kinetochore, Excitation: 505 / 569, Emission: 516 / 581
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 57386 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonemEos2-C1
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHec1
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Alt nameNM_006101.2
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SpeciesH. sapiens (human)
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Entrez GeneNDC80 (a.k.a. HEC, HEC1, HsHec1, KNTC2, TID3, hsNDC80)
- Promoter CMV
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Tag
/ Fusion Protein
- mEos2 (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 505 / 569; Emission = 516 / 581
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mEos2-Hec1-C-12 was a gift from Michael Davidson (Addgene plasmid # 57386 ; http://n2t.net/addgene:57386 ; RRID:Addgene_57386) -
For your References section:
A bright and photostable photoconvertible fluorescent protein. McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL. Nat Methods. 2009 Feb . 6(2):131-3. 10.1038/nmeth.1296 PubMed 19169260
