pJG/ Inducible ALPHA MHC
(Plasmid #55792)

• Purpose: (Empty Backbone) transgene expression driven by inducible ALPHA MHC promoter
• Depositing Lab: Jeffrey Robbins
• Publication: Sanbe et al Circ Res. 2003 Apr 4;92(6):609-16. Epub 2003 Mar 6.

Backbone

• Vector backbone: pBluescript II SK+
• Vector type: Mammalian Expression
• Promoter: Inducible ALPHA MHC

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13
• 3′ sequencing primer: M13R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The distance between the two Not1 sites is ~6kb
Clone into the Sal1 site (or Sal/Hind3 site).
Release the pBluescript backbone with Not1 for transgenics.
This construct consists of a full-length α-MHC promoter in which 3 GATA sites and 2 TREs were ablated. Other cis-acting regions important for cardiac-specific expression were left intact. To make the attenuated promoter Tet responsive, 7 repeats of the TetO sequences were inserted adjacent to the TATA box. The human growth hormone polyadenylation signal was placed downstream from the unique cloning site. Any gene of interest inserted into this Tet-responsive promoter construct will be inducible.