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Addgene

EBFP2-PCNA-19-SV40NLS-4
(Plasmid #55250)

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Item Catalog # Description Quantity Price (USD)
Plasmid 55250 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    EBFP2
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PCNA-19 SV40NLS
  • Alt name
    Human Proliferating Cell Nuclear Antigen + SV40 NLS 3x Repeat
  • Alt name
    NM_002592.2
  • Species
    H. sapiens (human)
  • Entrez Gene
    PCNA (a.k.a. ATLD2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • EBFP2 (N terminal on backbone)
    • 3xSV40 NLS (N terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

H. sapiens (human) Proliferating Cell Nuclear Antigen + SV40 NLS 3x Repeat. Excitation = 383; Emission = 448

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EBFP2-PCNA-19-SV40NLS-4 was a gift from Michael Davidson (Addgene plasmid # 55250 ; http://n2t.net/addgene:55250 ; RRID:Addgene_55250)