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Purpose(Empty Backbone) Localization: N1 Cloning Vector, Excitation: 434, Emission: 474
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54843 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonemTurquoise2-N1
- Backbone size (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
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Tag
/ Fusion Protein
- mTurquoise2 (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- mTurquoise2 (C terminal on backbone)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 434; Emission = 474
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mTurquoise2-N1 was a gift from Michael Davidson & Dorus Gadella (Addgene plasmid # 54843 ; http://n2t.net/addgene:54843 ; RRID:Addgene_54843) -
For your References section:
Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%. Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen L, Hink MA, van Weeren L, Gadella TW Jr, Royant A. Nat Commun. 2012 Mar 20;3:751. doi: 10.1038/ncomms1738. 10.1038/ncomms1738 PubMed 22434194
