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Addgene

IFP1.4-C1
(Plasmid #54782)

Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 54782 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    IFP1.4-C1
  • Backbone size (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)
  • Tag / Fusion Protein
    • IFP1.4 (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • IFP1.4 (N terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    R.Y. Tsien, PMID 19423828

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Excitation = 684; Emission = 708

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IFP1.4-C1 was a gift from Michael Davidson & Roger Tsien (Addgene plasmid # 54782 ; http://n2t.net/addgene:54782 ; RRID:Addgene_54782)
  • For your References section:

    Mammalian expression of infrared fluorescent proteins engineered from a bacterial phytochrome. Shu X, Royant A, Lin MZ, Aguilera TA, Lev-Ram V, Steinbach PA, Tsien RY. Science. 2009 May 8;324(5928):804-7. doi: 10.1126/science.1168683. 10.1126/science.1168683 PubMed 19423828