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Purpose(Empty Backbone) Localization: C1 Cloning Vector, Excitation: 507 / 583, Emission: 517 / 593
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54656 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonemKikGR-C1
- Backbone size (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
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Tag
/ Fusion Protein
- mKikGR (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- mKikGR (N terminal on backbone)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byA. Miyawaki, PMID 19079591
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 507 / 583; Emission = 517 / 593
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mKikGR-C1 was a gift from Michael Davidson & Atsushi Miyawaki (Addgene plasmid # 54656 ; http://n2t.net/addgene:54656 ; RRID:Addgene_54656) -
For your References section:
mKikGR, a monomeric photoswitchable fluorescent protein. Habuchi S, Tsutsui H, Kochaniak AB, Miyawaki A, van Oijen AM. PLoS One. 2008;3(12):e3944. doi: 10.1371/journal.pone.0003944. Epub 2008 Dec 15. 10.1371/journal.pone.0003944 PubMed 19079591
