CAG-lox-CAT-lox-unique Bam cloning site-hGH polyA (Clone 17)
(Plasmid #53959)

• Purpose: (Empty Backbone) allows Cre-mediated expression of transgene
• Depositing Lab: Jeffrey Robbins
• Publication: Nakamura et al Proc Natl Acad Sci U S A. 2009 Sep 8;106(36):15436-41. doi: 10.1073/pnas.0903302106. Epub 2009 Aug 24.

Backbone

• Vector backbone: pBluescript2 SK+
• Modifications to backbone: see author map
• Vector type: Mammalian Expression, Cre/Lox
• Promoter: CMV enhancer, chicken actin promoter
• Tag / Fusion Protein:
  • Lox-CAT-Lox (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13
• 3′ sequencing primer: M13R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

When the CAT gene is excised by Cre-mediated recombination between the tandem loxP sites, the gene of interest becomes positioned adjacent to the CAG promoter, permitting gene expression.