pHRIG-AktDN
(Plasmid #53597)

• Purpose: lentiviral expression of dominant negative mouse Akt1 and EGFP
• Depositing Lab: Heng Zhao
• Publication: Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12):1875-85. doi: 10.1038/jcbfm.2013.132. Epub 2013 Aug 14.

Backbone

• Vector backbone: pHR-IG (pHR’tripCMV-IRES-eGFP)
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Akt1
• Alt name: Akt1 DN
• Species: M. musculus (mouse)
• Mutation: Dominant negative (K179M)
• Entrez Gene: Akt1 (a.k.a. Akt, LTR-akt, PKB, PKB/Akt, PKBalpha, Rac)
• Promoter: CMV
• Tags / Fusion Proteins:
  • HA (N terminal on insert)
  • IRES-EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: pCDH-rev

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

AktDN was purchased from Addgene (plasmid # 16243).
The AktDN gene is an Akt gene where a point mutation in the 179th amino acid from the N terminal converts a lysine to a methionine. This mutated form of the Akt protein can still be phosphorylated at the S473 site but loses its ability to affect downstream factors.