pHBFD1
(Plasmid
#53565)
-
PurposeProduces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 53565 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBluescript SK-
-
Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2958
- Total vector size (bp) 4326
-
Modifications to backboneThis is a cDNA library plasmid from an Adonis aestivalis flower cDNA library.
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)XL1 Blue
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namehbfd1 cDNA
-
Alt nameAdKC28
-
Alt namecarotenoid 4-hydroxy-β-ring 4-dehydrogenase
-
SpeciesAdonis aestivalis
-
Insert Size (bp)1387
-
GenBank IDDQ902556.1
- Promoter lac
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHBFD1 was a gift from Francis X Cunningham Jr (Addgene plasmid # 53565 ; http://n2t.net/addgene:53565 ; RRID:Addgene_53565) -
For your References section:
Elucidation of the pathway to astaxanthin in the flowers of Adonis aestivalis. Cunningham FX Jr, Gantt E. Plant Cell. 2011 Aug;23(8):3055-69. doi: 10.1105/tpc.111.086827. Epub 2011 Aug 23. 10.1105/tpc.111.086827 PubMed 21862704