Skip to main content
Addgene

pHBFD1
(Plasmid #53565)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53565 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript SK-
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2958
  • Total vector size (bp) 4326
  • Modifications to backbone
    This is a cDNA library plasmid from an Adonis aestivalis flower cDNA library.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hbfd1 cDNA
  • Alt name
    AdKC28
  • Alt name
    carotenoid 4-hydroxy-β-ring 4-dehydrogenase
  • Species
    Adonis aestivalis
  • Insert Size (bp)
    1387
  • GenBank ID
    DQ902556.1
  • Promoter lac

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHBFD1 was a gift from Francis X Cunningham Jr (Addgene plasmid # 53565 ; http://n2t.net/addgene:53565 ; RRID:Addgene_53565)
  • For your References section:

    Elucidation of the pathway to astaxanthin in the flowers of Adonis aestivalis. Cunningham FX Jr, Gantt E. Plant Cell. 2011 Aug;23(8):3055-69. doi: 10.1105/tpc.111.086827. Epub 2011 Aug 23. 10.1105/tpc.111.086827 PubMed 21862704