pP[RS3]3'M
(Plasmid #53552)

• Purpose: for an easy screen of TALEN- and CRISPR/Cas9- mediated mutagenesis in Drosophila
• Depositing Lab: Renjie Jiao
• Publication: Yu et al Biol Open. 2014 Apr 15;3(4):271-80. doi: 10.1242/bio.20147682.

Backbone

• Vector backbone: pP[RS3]
• Backbone manufacturer: constructed by Kent G. Golic; offered by Drosophila Genomics Resource Center
• Backbone size w/o insert (bp): 8700
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AscI and MluI restriction enzyme sites
• Insert Size (bp): 714
• Promoter: same pP[RS3]

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bsp1407 (not destroyed)
• 3′ cloning site: Bsp1407 (not destroyed)
• 5′ sequencing primer: CTCAAATGGTTCGAGTGGT

Resource Information

• Supplemental Documents:
  • map of pPRS3M
• A portion of this plasmid was derived from a plasmid made by: The pP[RS3] plasmid was constructed by Kent G. Golic and we obtained it from Drosophila Genomics Resource Center.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry