pNW3
(Plasmid #53372)

• Purpose: Csy4 and Cas9 D10A nickase expression plasmid
• Depositing Lab: Keith Joung
• Publication: Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.

Backbone

• Vector backbone: pCAG-CFP
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Csy4-T2A-Cas9n (D10A)
• Alt name: Csy4
• Alt name: Cas9 nickase (D10A)
• Promoter: CAG

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: Bglob-pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry