pEXP5-pSoxS::mCherry
(Plasmid
#53311)
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PurposemCherry reporter plasmid for SoxS promoter
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 53311 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEXP5-NT/TOPO
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Backbone manufacturerInvitrogen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSoxS promoter
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SpeciesE.coli
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Insert Size (bp)805
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Entrez GenesoxS (a.k.a. b4062, ECK4054)
- Promoter T7
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Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal or mCherry-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEXP5-pSoxS::mCherry was a gift from William DeGrado (Addgene plasmid # 53311 ; http://n2t.net/addgene:53311 ; RRID:Addgene_53311)