pTRC-APX(W41F) dimeric
(Plasmid #58499)

• Purpose: Bacterial expression of APX W41F inducible by IPTG
• Depositing Lab: Alice Ting
• Publication: Dwyer et al Proc Natl Acad Sci U S A. 2014 May 20;111(20):E2100-9. doi: 10.1073/pnas.1401876111. Epub 2014 May 6.

Backbone

• Vector backbone: pTRC
• Backbone size w/o insert (bp): 4188
• Total vector size (bp): 4938
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pea APX W41F (dimeric)
• Species: Synthetic
• Insert Size (bp): 747
• Mutation: W41F
• Promoter: trc
• Tag / Fusion Protein:
  • His6 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pTRC-fwd
• 3′ sequencing primer: pTRC-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid encodes the W41F mutant of pea ascorbate peroxidase (APX). The W41F mutation improves enzyme activity. APX W41F is a constitutive homodimer, unlike "APEX", so APX W41F should not be used as a fusion to proteins that are sensitive to dimeric tags.