pJM31
(Plasmid #52986)

• Purpose: A secreted Annexin V (sAnxV::GFP) Phosphatidylserine sensor
• Depositing Lab: Ding Xue
• Publication: Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. doi: 10.1016/j.cub.2012.05.052. Epub 2012 Jun 21.

Backbone

• Vector backbone: pPD 49.83
• Backbone size w/o insert (bp): 4710
• Total vector size (bp): 6648
• Modifications to backbone: duplicated unc-54 3' utr
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Annexin V
• Species: H. sapiens (human)
• Entrez Gene: ANXA5 (a.k.a. ANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph)
• Promoter: hsp-16.41
• Tags / Fusion Proteins:
  • GFP (w/ introns) (C terminal on insert)
  • signal sequence (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: hsp-16
• 3′ sequencing primer: unc-54

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry