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PurposeExpresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52970 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneJDS246
- Backbone size w/o insert (bp) 3400
- Total vector size (bp) 8271
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFok1 fused to dCas9
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Insert Size (bp)4800
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MutationD10A and H840A in Cas9
- Promoter CMV
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Tags
/ Fusion Proteins
- NLS (N terminal on insert)
- Fok1 nuclease (homodimeric wildtype) (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer TAATACGACTCACTATAGG
- 3′ sequencing primer CAGGGTCAAGGAAGGCACG (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FokI-dCas9 was a gift from David Liu (Addgene plasmid # 52970 ; http://n2t.net/addgene:52970 ; RRID:Addgene_52970) -
For your References section:
Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification. Guilinger JP, Thompson DB, Liu DR. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2909. 10.1038/nbt.2909 PubMed 24770324
Map uploaded by the depositor.
