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lentiGuide-Puro Citations (560)

Originally described in: Improved vectors and genome-wide libraries for CRISPR screening.
Sanjana NE, Shalem O, Zhang F Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. PubMed Journal

Articles Citing lentiGuide-Puro

Articles
Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. PubMed

Associated Plasmids
Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Doench JG, Hartenian E, Graham DB, Tothova Z, Hegde M, Smith I, Sullender M, Ebert BL, Xavier RJ, Root DE. Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026. PubMed

Associated Plasmids
TRAF1 Coordinates Polyubiquitin Signaling to Enhance Epstein-Barr Virus LMP1-Mediated Growth and Survival Pathway Activation. Greenfeld H, Takasaki K, Walsh MJ, Ersing I, Bernhardt K, Ma Y, Fu B, Ashbaugh CW, Cabo J, Mollo SB, Zhou H, Li S, Gewurz BE. PLoS Pathog. 2015 May 21;11(5):e1004890. doi: 10.1371/journal.ppat.1004890. eCollection 2015 May. PubMed
Cell death during crisis is mediated by mitotic telomere deprotection. Hayashi MT, Cesare AJ, Rivera T, Karlseder J. Nature. 2015 Jun 25;522(7557):492-6. doi: 10.1038/nature14513. PubMed
A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks. Parnas O, Jovanovic M, Eisenhaure TM, Herbst RH, Dixit A, Ye CJ, Przybylski D, Platt RJ, Tirosh I, Sanjana NE, Shalem O, Satija R, Raychowdhury R, Mertins P, Carr SA, Zhang F, Hacohen N, Regev A. Cell. 2015 Jul 30;162(3):675-86. doi: 10.1016/j.cell.2015.06.059. Epub 2015 Jul 16. PubMed
BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis. Canver MC, Smith EC, Sher F, Pinello L, Sanjana NE, Shalem O, Chen DD, Schupp PG, Vinjamur DS, Garcia SP, Luc S, Kurita R, Nakamura Y, Fujiwara Y, Maeda T, Yuan GC, Zhang F, Orkin SH, Bauer DE. Nature. 2015 Sep 16. doi: 10.1038/nature15521. PubMed

Associated Plasmids
Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease. Dahlman JE, Abudayyeh OO, Joung J, Gootenberg JS, Zhang F, Konermann S. Nat Biotechnol. 2015 Oct 5;33(11):1159-1161. doi: 10.1038/nbt.3390. PubMed
DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs. Aparicio-Prat E, Arnan C, Sala I, Bosch N, Guigo R, Johnson R. BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z. PubMed

Associated Plasmids
The NF-kappaB subunit c-Rel regulates Bach2 tumour suppressor expression in B-cell lymphoma. Hunter JE, Butterworth JA, Zhao B, Sellier H, Campbell KJ, Thomas HD, Bacon CM, Cockell SJ, Gewurz BE, Perkins ND. Oncogene. 2015 Nov 2. doi: 10.1038/onc.2015.399. PubMed
Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers. Zhang X, Choi PS, Francis JM, Imielinski M, Watanabe H, Cherniack AD, Meyerson M. Nat Genet. 2016 Feb;48(2):176-82. doi: 10.1038/ng.3470. Epub 2015 Dec 14. PubMed
Enhanced Store-Operated Calcium Entry Leads to Striatal Synaptic Loss in a Huntington's Disease Mouse Model. Wu J, Ryskamp DA, Liang X, Egorova P, Zakharova O, Hung G, Bezprozvanny I. J Neurosci. 2016 Jan 6;36(1):125-41. doi: 10.1523/JNEUROSCI.1038-15.2016. PubMed
CRISPR-Cas9 Can Inhibit HIV-1 Replication but NHEJ Repair Facilitates Virus Escape. Wang G, Zhao N, Berkhout B, Das AT. Mol Ther. 2016 Mar;24(3):522-6. doi: 10.1038/mt.2016.24. Epub 2016 Jan 22. PubMed
Single Guide RNA Library Design and Construction. Wang T, Lander ES, Sabatini DM. Cold Spring Harb Protoc. 2016 Mar 1;2016(3):pdb.prot090803. doi: 10.1101/pdb.prot090803. PubMed
Mutant Calreticulin Requires Both Its Mutant C-terminus and the Thrombopoietin Receptor for Oncogenic Transformation. Elf S, Abdelfattah NS, Chen E, Perales-Paton J, Rosen EA, Ko A, Peisker F, Florescu N, Giannini S, Wolach O, Morgan EA, Tothova Z, Losman JA, Schneider RK, Al-Shahrour F, Mullally A. Cancer Discov. 2016 Apr;6(4):368-81. doi: 10.1158/2159-8290.CD-15-1434. Epub 2016 Mar 7. PubMed
Novel Small-Molecule CX3CR1 Antagonist Impairs Metastatic Seeding and Colonization of Breast Cancer Cells. Shen F, Zhang Y, Jernigan DL, Feng X, Yan J, Garcia FU, Meucci O, Salvino JM, Fatatis A. Mol Cancer Res. 2016 Jun;14(6):518-27. doi: 10.1158/1541-7786.MCR-16-0013. Epub 2016 Mar 21. PubMed
CRISPR knockout screening outperforms shRNA and CRISPRi in identifying essential genes. Evers B, Jastrzebski K, Heijmans JP, Grernrum W, Beijersbergen RL, Bernards R. Nat Biotechnol. 2016 Jun;34(6):631-3. doi: 10.1038/nbt.3536. Epub 2016 Apr 25. PubMed
RBFOX1 and RBFOX2 are dispensable in iPSCs and iPSC-derived neurons and do not contribute to neural-specific paternal UBE3A silencing. Chen PF, Hsiao JS, Sirois CL, Chamberlain SJ. Sci Rep. 2016 May 5;6:25368. doi: 10.1038/srep25368. PubMed
A human genome-wide loss-of-function screen identifies effective chikungunya antiviral drugs. Karlas A, Berre S, Couderc T, Varjak M, Braun P, Meyer M, Gangneux N, Karo-Astover L, Weege F, Raftery M, Schonrich G, Klemm U, Wurzlbauer A, Bracher F, Merits A, Meyer TF, Lecuit M. Nat Commun. 2016 May 12;7:11320. doi: 10.1038/ncomms11320. PubMed
Modeling invasive lobular breast carcinoma by CRISPR/Cas9-mediated somatic genome editing of the mammary gland. Annunziato S, Kas SM, Nethe M, Yucel H, Del Bravo J, Pritchard C, Bin Ali R, van Gerwen B, Siteur B, Drenth AP, Schut E, van de Ven M, Boelens MC, Klarenbeek S, Huijbers IJ, van Miltenburg MH, Jonkers J. Genes Dev. 2016 Jun 15;30(12):1470-80. doi: 10.1101/gad.279190.116. PubMed
A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis. Lambrus BG, Daggubati V, Uetake Y, Scott PM, Clutario KM, Sluder G, Holland AJ. J Cell Biol. 2016 Jul 18;214(2):143-53. doi: 10.1083/jcb.201604054. PubMed
Treatment with an SLC12A1 antagonist inhibits tumorigenesis in a subset of hepatocellular carcinomas. Teng F, Guo M, Liu F, Wang C, Dong J, Zhang L, Zou Y, Chen R, Sun K, Fu H, Fu Z, Guo W, Ding G. Oncotarget. 2016 Aug 16;7(33):53571-53582. doi: 10.18632/oncotarget.10670. PubMed
Histone H3K27 Trimethylation Modulates 5-Fluorouracil Resistance by Inhibiting PU.1 Binding to the DPYD Promoter. Wu R, Nie Q, Tapper EE, Jerde CR, Dunlap GS, Shrestha S, Elraiyah TA, Offer SM, Diasio RB. Cancer Res. 2016 Nov 1;76(21):6362-6373. Epub 2016 Aug 30. PubMed
Challenges of CRISPR/Cas9 applications for long non-coding RNA genes. Goyal A, Myacheva K, Gross M, Klingenberg M, Duran Arque B, Diederichs S. Nucleic Acids Res. 2016 Sep 30. pii: gkw883. PubMed
Orphan G protein-coupled receptor GPRC5A modulates integrin beta1-mediated epithelial cell adhesion. Bulanova DR, Akimov YA, Rokka A, Laajala TD, Aittokallio T, Kouvonen P, Pellinen T, Kuznetsov SG. Cell Adh Migr. 2016 Oct 7:0. PubMed
The sigma-1 receptor mediates the beneficial effects of pridopidine in a mouse model of Huntington disease. Ryskamp D, Wu J, Geva M, Kusko R, Grossman I, Hayden M, Bezprozvanny I. Neurobiol Dis. 2017 Jan;97(Pt A):46-59. doi: 10.1016/j.nbd.2016.10.006. Epub 2016 Nov 3. PubMed
DPP8 and DPP9 inhibition induces pro-caspase-1-dependent monocyte and macrophage pyroptosis. Okondo MC, Johnson DC, Sridharan R, Go EB, Chui AJ, Wang MS, Poplawski SE, Wu W, Liu Y, Lai JH, Sanford DG, Arciprete MO, Golub TR, Bachovchin WW, Bachovchin DA. Nat Chem Biol. 2017 Jan;13(1):46-53. doi: 10.1038/nchembio.2229. Epub 2016 Nov 7. PubMed
Alternative splicing of EZH2 pre-mRNA by SF3B3 contributes to the tumorigenic potential of renal cancer. Chen K, Xiao H, Zeng J, Yu G, Zhou H, Huang C, Yao W, Xiao W, Hu J, Guan W, Wu L, Huang J, Huang Q, Xu H, Ye Z. Clin Cancer Res. 2016 Nov 22. pii: clincanres.2020.2016. PubMed
A Combinatorial CRISPR-Cas9 Attack on HIV-1 DNA Extinguishes All Infectious Provirus in Infected T Cell Cultures. Wang G, Zhao N, Berkhout B, Das AT. Cell Rep. 2016 Dec 13;17(11):2819-2826. doi: 10.1016/j.celrep.2016.11.057. PubMed
Dissecting Immune Circuits by Linking CRISPR-Pooled Screens with Single-Cell RNA-Seq. Jaitin DA, Weiner A, Yofe I, Lara-Astiaso D, Keren-Shaul H, David E, Salame TM, Tanay A, van Oudenaarden A, Amit I. Cell. 2016 Dec 15;167(7):1883-1896.e15. doi: 10.1016/j.cell.2016.11.039. PubMed

Associated Plasmids
In vivo high-throughput profiling of CRISPR-Cpf1 activity. Kim HK, Song M, Lee J, Menon AV, Jung S, Kang YM, Choi JW, Woo E, Koh HC, Nam JW, Kim H. Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104. PubMed

Associated Plasmids
MAPK signaling promotes axonal degeneration by speeding the turnover of the axonal maintenance factor NMNAT2. Walker LJ, Summers DW, Sasaki Y, Brace EJ, Milbrandt J, DiAntonio A. Elife. 2017 Jan 17;6. doi: 10.7554/eLife.22540. PubMed
Pooled CRISPR screening with single-cell transcriptome readout. Datlinger P, Rendeiro AF, Schmidl C, Krausgruber T, Traxler P, Klughammer J, Schuster LC, Kuchler A, Alpar D, Bock C. Nat Methods. 2017 Jan 18. doi: 10.1038/nmeth.4177. PubMed

Associated Plasmids
Challenges of CRISPR/Cas9 applications for long non-coding RNA genes. Goyal A, Myacheva K, Gross M, Klingenberg M, Duran Arque B, Diederichs S. Nucleic Acids Res. 2017 Feb 17;45(3):e12. doi: 10.1093/nar/gkw883. PubMed
Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci. Canver MC, Lessard S, Pinello L, Wu Y, Ilboudo Y, Stern EN, Needleman AJ, Galacteros F, Brugnara C, Kutlar A, McKenzie C, Reid M, Chen DD, Das PP, A Cole M, Zeng J, Kurita R, Nakamura Y, Yuan GC, Lettre G, Bauer DE, Orkin SH. Nat Genet. 2017 Feb 20. doi: 10.1038/ng.3793. PubMed

Associated Plasmids
TIRR regulates 53BP1 by masking its histone methyl-lysine binding function. Drane P, Brault ME, Cui G, Meghani K, Chaubey S, Detappe A, Parnandi N, He Y, Zheng XF, Botuyan MV, Kalousi A, Yewdell WT, Munch C, Harper JW, Chaudhuri J, Soutoglou E, Mer G, Chowdhury D. Nature. 2017 Mar 9;543(7644):211-216. doi: 10.1038/nature21358. Epub 2017 Feb 27. PubMed
Glucose Deprivation Induces ATF4-Mediated Apoptosis through TRAIL Death Receptors. Iurlaro R, Puschel F, Leon-Annicchiarico CL, O'Connor H, Martin SJ, Palou-Gramon D, Lucendo E, Munoz-Pinedo C. Mol Cell Biol. 2017 May 2;37(10). pii: e00479-16. doi: 10.1128/MCB.00479-16. Print 2017 May 15. PubMed
Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions. Shen JP, Zhao D, Sasik R, Luebeck J, Birmingham A, Bojorquez-Gomez A, Licon K, Klepper K, Pekin D, Beckett AN, Sanchez KS, Thomas A, Kuo CC, Du D, Roguev A, Lewis NE, Chang AN, Kreisberg JF, Krogan N, Qi L, Ideker T, Mali P. Nat Methods. 2017 Jun;14(6):573-576. doi: 10.1038/nmeth.4225. Epub 2017 Mar 20. PubMed
Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. Epub 2017 Mar 23. PubMed

Associated Plasmids
USP9X regulates centrosome duplication and promotes breast carcinogenesis. Li X, Song N, Liu L, Liu X, Ding X, Song X, Yang S, Shan L, Zhou X, Su D, Wang Y, Zhang Q, Cao C, Ma S, Yu N, Yang F, Wang Y, Yao Z, Shang Y, Shi L. Nat Commun. 2017 Mar 31;8:14866. doi: 10.1038/ncomms14866. PubMed
Pyroptosis and Apoptosis Pathways Engage in Bidirectional Crosstalk in Monocytes and Macrophages. Taabazuing CY, Okondo MC, Bachovchin DA. Cell Chem Biol. 2017 Apr 20;24(4):507-514.e4. doi: 10.1016/j.chembiol.2017.03.009. Epub 2017 Apr 6. PubMed
Epstein-Barr virus ensures B cell survival by uniquely modulating apoptosis at early and late times after infection. Price AM, Dai J, Bazot Q, Patel L, Nikitin PA, Djavadian R, Winter PS, Salinas CA, Barry AP, Wood KC, Johannsen EC, Letai A, Allday MJ, Luftig MA. Elife. 2017 Apr 20;6. pii: e22509. doi: 10.7554/eLife.22509. PubMed
Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation. Hung V, Lam SS, Udeshi ND, Svinkina T, Guzman G, Mootha VK, Carr SA, Ting AY. Elife. 2017 Apr 25;6. pii: e24463. doi: 10.7554/eLife.24463. PubMed

Associated Plasmids
High-content analysis screening for cell cycle regulators using arrayed synthetic crRNA libraries. Strezoska Z, Perkett MR, Chou ET, Maksimova E, Anderson EM, McClelland S, Kelley ML, Vermeulen A, Smith AVB. J Biotechnol. 2017 Jun 10;251:189-200. doi: 10.1016/j.jbiotec.2017.04.017. Epub 2017 Apr 23. PubMed
Genome-wide association study of red blood cell traits in Hispanics/Latinos: The Hispanic Community Health Study/Study of Latinos. Hodonsky CJ, Jain D, Schick UM, Morrison JV, Brown L, McHugh CP, Schurmann C, Chen DD, Liu YM, Auer PL, Laurie CA, Taylor KD, Browning BL, Li Y, Papanicolaou G, Rotter JI, Kurita R, Nakamura Y, Browning SR, Loos RJF, North KE, Laurie CC, Thornton TA, Pankratz N, Bauer DE, Sofer T, Reiner AP. PLoS Genet. 2017 Apr 28;13(4):e1006760. doi: 10.1371/journal.pgen.1006760. eCollection 2017 Apr. PubMed
CRISPR/Cas9 Screens Reveal Epstein-Barr Virus-Transformed B Cell Host Dependency Factors. Ma Y, Walsh MJ, Bernhardt K, Ashbaugh CW, Trudeau SJ, Ashbaugh IY, Jiang S, Jiang C, Zhao B, Root DE, Doench JG, Gewurz BE. Cell Host Microbe. 2017 May 10;21(5):580-591.e7. doi: 10.1016/j.chom.2017.04.005. PubMed
LDB1-mediated enhancer looping can be established independent of mediator and cohesin. Krivega I, Dean A. Nucleic Acids Res. 2017 May 18. doi: 10.1093/nar/gkx433. PubMed
Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression. Rosenbluh J, Xu H, Harrington W, Gill S, Wang X, Vazquez F, Root DE, Tsherniak A, Hahn WC. Nat Commun. 2017 May 23;8:15403. doi: 10.1038/ncomms15403. PubMed

Associated Plasmids
The ubiquitin ligase STUB1 regulates stability and activity of RUNX1 and RUNX1-RUNX1T1. Yonezawa T, Takahashi H, Shikata S, Liu X, Tamura M, Asada S, Fukushima T, Fukuyama T, Tanaka Y, Sawasaki T, Kitamura T, Goyama S. J Biol Chem. 2017 Jul 28;292(30):12528-12541. doi: 10.1074/jbc.M117.785675. Epub 2017 May 23. PubMed
RNA-Guided Activation of Pluripotency Genes in Human Fibroblasts. Xiong K, Zhou Y, Blichfeld KA, Hyttel P, Bolund L, Freude KK, Luo Y. Cell Reprogram. 2017 Jun;19(3):189-198. doi: 10.1089/cell.2017.0006. PubMed
Aldolase B Overexpression is Associated with Poor Prognosis and Promotes Tumor Progression by Epithelial-Mesenchymal Transition in Colorectal Adenocarcinoma. Li Q, Li Y, Xu J, Wang S, Xu Y, Li X, Cai S. Cell Physiol Biochem. 2017;42(1):397-406. doi: 10.1159/000477484. Epub 2017 May 25. PubMed
Seed-effect modeling improves the consistency of genome-wide loss-of-function screens and identifies synthetic lethal vulnerabilities in cancer cells. Jaiswal A, Peddinti G, Akimov Y, Wennerberg K, Kuznetsov S, Tang J, Aittokallio T. Genome Med. 2017 Jun 1;9(1):51. doi: 10.1186/s13073-017-0440-2. PubMed
Chromatin accessibility and guide sequence secondary structure affect CRISPR-Cas9 gene editing efficiency. Jensen KT, Floe L, Petersen TS, Huang J, Xu F, Bolund L, Luo Y, Lin L. FEBS Lett. 2017 Jul;591(13):1892-1901. doi: 10.1002/1873-3468.12707. Epub 2017 Jun 28. PubMed
CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion. Mou H, Smith JL, Peng L, Yin H, Moore J, Zhang XO, Song CQ, Sheel A, Wu Q, Ozata DM, Li Y, Anderson DG, Emerson CP, Sontheimer EJ, Moore MJ, Weng Z, Xue W. Genome Biol. 2017 Jun 14;18(1):108. doi: 10.1186/s13059-017-1237-8. PubMed
Optogenetic activation of Plexin-B1 reveals contact repulsion between osteoclasts and osteoblasts. Deb Roy A, Yin T, Choudhary S, Rodionov V, Pilbeam CC, Wu YI. Nat Commun. 2017 Jun 21;8:15831. doi: 10.1038/ncomms15831. PubMed
A module for Rac temporal signal integration revealed with optogenetics. Graziano BR, Gong D, Anderson KE, Pipathsouk A, Goldberg AR, Weiner OD. J Cell Biol. 2017 Aug 7;216(8):2515-2531. doi: 10.1083/jcb.201604113. Epub 2017 Jul 7. PubMed
CRISPR/Cas9-Mediated Scanning for Regulatory Elements Required for HPRT1 Expression via Thousands of Large, Programmed Genomic Deletions. Gasperini M, Findlay GM, McKenna A, Milbank JH, Lee C, Zhang MD, Cusanovich DA, Shendure J. Am J Hum Genet. 2017 Aug 3;101(2):192-205. doi: 10.1016/j.ajhg.2017.06.010. Epub 2017 Jul 14. PubMed
An erythroid-specific ATP2B4 enhancer mediates red blood cell hydration and malaria susceptibility. Lessard S, Gatof ES, Beaudoin M, Schupp PG, Sher F, Ali A, Prehar S, Kurita R, Nakamura Y, Baena E, Ledoux J, Oceandy D, Bauer DE, Lettre G. J Clin Invest. 2017 Aug 1;127(8):3065-3074. doi: 10.1172/JCI94378. Epub 2017 Jul 17. PubMed
Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes. Ho SM, Hartley BJ, Flaherty E, Rajarajan P, Abdelaal R, Obiorah I, Barretto N, Muhammad H, Phatnani HP, Akbarian S, Brennand KJ. Stem Cell Reports. 2017 Aug 8;9(2):615-628. doi: 10.1016/j.stemcr.2017.06.012. Epub 2017 Jul 27. PubMed

Associated Plasmids
Technical considerations for the use of CRISPR/Cas9 in hematology research. Gundry MC, Dever DP, Yudovich D, Bauer DE, Haas S, Wilkinson AC, Singbrant S. Exp Hematol. 2017 Oct;54:4-11. doi: 10.1016/j.exphem.2017.07.006. Epub 2017 Jul 27. PubMed
Mitotic progression following DNA damage enables pattern recognition within micronuclei. Harding SM, Benci JL, Irianto J, Discher DE, Minn AJ, Greenberg RA. Nature. 2017 Aug 24;548(7668):466-470. doi: 10.1038/nature23470. Epub 2017 Jul 31. PubMed
Decomposing Oncogenic Transcriptional Signatures to Generate Maps of Divergent Cellular States. Kim JW, Abudayyeh OO, Yeerna H, Yeang CH, Stewart M, Jenkins RW, Kitajima S, Konieczkowski DJ, Medetgul-Ernar K, Cavazos T, Mah C, Ting S, Van Allen EM, Cohen O, Mcdermott J, Damato E, Aguirre AJ, Liang J, Liberzon A, Alexe G, Doench J, Ghandi M, Vazquez F, Weir BA, Tsherniak A, Subramanian A, Meneses-Cime K, Park J, Clemons P, Garraway LA, Thomas D, Boehm JS, Barbie DA, Hahn WC, Mesirov JP, Tamayo P. Cell Syst. 2017 Aug 23;5(2):105-118.e9. doi: 10.1016/j.cels.2017.08.002. PubMed
Combinatorial CRISPR-Cas9 and RNA Interference Attack on HIV-1 DNA and RNA Can Lead to Cross-Resistance. Zhao N, Wang G, Das AT, Berkhout B. Antimicrob Agents Chemother. 2017 Nov 22;61(12). pii: AAC.01486-17. doi: 10.1128/AAC.01486-17. Print 2017 Dec. PubMed
BRCA2 suppresses replication stress-induced mitotic and G1 abnormalities through homologous recombination. Feng W, Jasin M. Nat Commun. 2017 Sep 13;8(1):525. doi: 10.1038/s41467-017-00634-0. PubMed
Genome-Wide CRISPR Screen for Essential Cell Growth Mediators in Mutant KRAS Colorectal Cancers. Yau EH, Kummetha IR, Lichinchi G, Tang R, Zhang Y, Rana TM. Cancer Res. 2017 Nov 15;77(22):6330-6339. doi: 10.1158/0008-5472.CAN-17-2043. Epub 2017 Sep 27. PubMed
CRISPR Genome-Wide Screening Identifies Dependence on the Proteasome Subunit PSMC6 for Bortezomib Sensitivity in Multiple Myeloma. Shi CX, Kortum KM, Zhu YX, Bruins LA, Jedlowski P, Votruba PG, Luo M, Stewart RA, Ahmann J, Braggio E, Stewart AK. Mol Cancer Ther. 2017 Dec;16(12):2862-2870. doi: 10.1158/1535-7163.MCT-17-0130. Epub 2017 Sep 27. PubMed
Somatic Superenhancer Duplications and Hotspot Mutations Lead to Oncogenic Activation of the KLF5 Transcription Factor. Zhang X, Choi PS, Francis JM, Gao GF, Campbell JD, Ramachandran A, Mitsuishi Y, Ha G, Shih J, Vazquez F, Tsherniak A, Taylor AM, Zhou J, Wu Z, Berger AC, Giannakis M, Hahn WC, Cherniack AD, Meyerson M. Cancer Discov. 2018 Jan;8(1):108-125. doi: 10.1158/2159-8290.CD-17-0532. Epub 2017 Sep 29. PubMed
The Glia-Neuron Lactate Shuttle and Elevated ROS Promote Lipid Synthesis in Neurons and Lipid Droplet Accumulation in Glia via APOE/D. Liu L, MacKenzie KR, Putluri N, Maletic-Savatic M, Bellen HJ. Cell Metab. 2017 Nov 7;26(5):719-737.e6. doi: 10.1016/j.cmet.2017.08.024. Epub 2017 Sep 28. PubMed
Genetic and Functional Drivers of Diffuse Large B Cell Lymphoma. Reddy A, Zhang J, Davis NS, Moffitt AB, Love CL, Waldrop A, Leppa S, Pasanen A, Meriranta L, Karjalainen-Lindsberg ML, Norgaard P, Pedersen M, Gang AO, Hogdall E, Heavican TB, Lone W, Iqbal J, Qin Q, Li G, Kim SY, Healy J, Richards KL, Fedoriw Y, Bernal-Mizrachi L, Koff JL, Staton AD, Flowers CR, Paltiel O, Goldschmidt N, Calaminici M, Clear A, Gribben J, Nguyen E, Czader MB, Ondrejka SL, Collie A, Hsi ED, Tse E, Au-Yeung RKH, Kwong YL, Srivastava G, Choi WWL, Evens AM, Pilichowska M, Sengar M, Reddy N, Li S, Chadburn A, Gordon LI, Jaffe ES, Levy S, Rempel R, Tzeng T, Happ LE, Dave T, Rajagopalan D, Datta J, Dunson DB, Dave SS. Cell. 2017 Oct 5;171(2):481-494.e15. doi: 10.1016/j.cell.2017.09.027. PubMed
CRISPR/Cas9 screening using unique molecular identifiers. Schmierer B, Botla SK, Zhang J, Turunen M, Kivioja T, Taipale J. Mol Syst Biol. 2017 Oct 9;13(10):945. doi: 10.15252/msb.20177834. PubMed

Associated Plasmids
The Epstein-Barr Virus Regulome in Lymphoblastoid Cells. Jiang S, Zhou H, Liang J, Gerdt C, Wang C, Ke L, Schmidt SCS, Narita Y, Ma Y, Wang S, Colson T, Gewurz B, Li G, Kieff E, Zhao B. Cell Host Microbe. 2017 Oct 11;22(4):561-573.e4. doi: 10.1016/j.chom.2017.09.001. PubMed
CRISPR/Cas9-mediated gene knockout is insensitive to target copy number but is dependent on guide RNA potency and Cas9/sgRNA threshold expression level. Yuen G, Khan FJ, Gao S, Stommel JM, Batchelor E, Wu X, Luo J. Nucleic Acids Res. 2017 Nov 16;45(20):12039-12053. doi: 10.1093/nar/gkx843. PubMed

Associated Plasmids
Systematic identification of regulatory variants associated with cancer risk. Liu S, Liu Y, Zhang Q, Wu J, Liang J, Yu S, Wei GH, White KP, Wang X. Genome Biol. 2017 Oct 23;18(1):194. doi: 10.1186/s13059-017-1322-z. PubMed
TUBB3 overexpression has a negligible effect on the sensitivity to taxol in cultured cell lines. Tame MA, Manjon AG, Belokhvostova D, Raaijmakers JA, Medema RH. Oncotarget. 2017 May 10;8(42):71536-71547. doi: 10.18632/oncotarget.17740. eCollection 2017 Sep 22. PubMed
Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines. Vinjamur DS, Bauer DE. Methods Mol Biol. 2018;1698:275-284. doi: 10.1007/978-1-4939-7428-3_17. PubMed
PD-1 is a haploinsufficient suppressor of T cell lymphomagenesis. Wartewig T, Kurgyis Z, Keppler S, Pechloff K, Hameister E, Ollinger R, Maresch R, Buch T, Steiger K, Winter C, Rad R, Ruland J. Nature. 2017 Dec 7;552(7683):121-125. doi: 10.1038/nature24649. Epub 2017 Nov 15. PubMed
Evaluation of RNAi and CRISPR technologies by large-scale gene expression profiling in the Connectivity Map. Smith I, Greenside PG, Natoli T, Lahr DL, Wadden D, Tirosh I, Narayan R, Root DE, Golub TR, Subramanian A, Doench JG. PLoS Biol. 2017 Nov 30;15(11):e2003213. doi: 10.1371/journal.pbio.2003213. eCollection 2017 Nov. PubMed
Epstein-Barr Virus Induces Adhesion Receptor CD226 (DNAM-1) Expression during Primary B-Cell Transformation into Lymphoblastoid Cell Lines. Grossman L, Chang C, Dai J, Nikitin PA, Jima DD, Dave SS, Luftig MA. mSphere. 2017 Nov 29;2(6). pii: mSphere00305-17. doi: 10.1128/mSphere.00305-17. eCollection 2017 Nov-Dec. PubMed
Lentiviral CRISPR/Cas9 nickase vector mediated BIRC5 editing inhibits epithelial to mesenchymal transition in ovarian cancer cells. Zhao G, Wang Q, Gu Q, Qiang W, Wei JJ, Dong P, Watari H, Li W, Yue J. Oncotarget. 2017 Oct 17;8(55):94666-94680. doi: 10.18632/oncotarget.21863. eCollection 2017 Nov 7. PubMed
YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Weintraub AS, Li CH, Zamudio AV, Sigova AA, Hannett NM, Day DS, Abraham BJ, Cohen MA, Nabet B, Buckley DL, Guo YE, Hnisz D, Jaenisch R, Bradner JE, Gray NS, Young RA. Cell. 2017 Dec 14;171(7):1573-1588.e28. doi: 10.1016/j.cell.2017.11.008. Epub 2017 Dec 7. PubMed

Associated Plasmids
Statin-Induced Cancer Cell Death Can Be Mechanistically Uncoupled from Prenylation of RAS Family Proteins. Yu R, Longo J, van Leeuwen JE, Mullen PJ, Ba-Alawi W, Haibe-Kains B, Penn LZ. Cancer Res. 2018 Mar 1;78(5):1347-1357. doi: 10.1158/0008-5472.CAN-17-1231. Epub 2017 Dec 11. PubMed
Therapeutic targeting of ependymoma as informed by oncogenic enhancer profiling. Mack SC, Pajtler KW, Chavez L, Okonechnikov K, Bertrand KC, Wang X, Erkek S, Federation A, Song A, Lee C, Wang X, McDonald L, Morrow JJ, Saiakhova A, Sin-Chan P, Wu Q, Michaelraj KA, Miller TE, Hubert CG, Ryzhova M, Garzia L, Donovan L, Dombrowski S, Factor DC, Luu B, Valentim CLL, Gimple RC, Morton A, Kim L, Prager BC, Lee JJY, Wu X, Zuccaro J, Thompson Y, Holgado BL, Reimand J, Ke SQ, Tropper A, Lai S, Vijayarajah S, Doan S, Mahadev V, Minan AF, Grobner SN, Lienhard M, Zapatka M, Huang Z, Aldape KD, Carcaboso AM, Houghton PJ, Keir ST, Milde T, Witt H, Li Y, Li CJ, Bian XW, Jones DTW, Scott I, Singh SK, Huang A, Dirks PB, Bouffet E, Bradner JE, Ramaswamy V, Jabado N, Rutka JT, Northcott PA, Lupien M, Lichter P, Korshunov A, Scacheri PC, Pfister SM, Kool M, Taylor MD, Rich JN. Nature. 2018 Jan 4;553(7686):101-105. doi: 10.1038/nature25169. Epub 2017 Dec 20. PubMed
CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog. Pusapati GV, Kong JH, Patel BB, Krishnan A, Sagner A, Kinnebrew M, Briscoe J, Aravind L, Rohatgi R. Dev Cell. 2018 Jan 8;44(1):113-129.e8. doi: 10.1016/j.devcel.2017.12.003. Epub 2017 Dec 28. PubMed
Ephrin receptor A2 is a functional entry receptor for Epstein-Barr virus. Chen J, Sathiyamoorthy K, Zhang X, Schaller S, Perez White BE, Jardetzky TS, Longnecker R. Nat Microbiol. 2018 Feb;3(2):172-180. doi: 10.1038/s41564-017-0081-7. Epub 2018 Jan 1. PubMed
Humanized TREM2 mice reveal microglia-intrinsic and -extrinsic effects of R47H polymorphism. Song WM, Joshita S, Zhou Y, Ulland TK, Gilfillan S, Colonna M. J Exp Med. 2018 Mar 5;215(3):745-760. doi: 10.1084/jem.20171529. Epub 2018 Jan 10. PubMed
PERK-mediated translational control is required for collagen secretion in chondrocytes. Hisanaga S, Miyake M, Taniuchi S, Oyadomari M, Morimoto M, Sato R, Hirose J, Mizuta H, Oyadomari S. Sci Rep. 2018 Jan 15;8(1):773. doi: 10.1038/s41598-017-19052-9. PubMed
In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice. Zhou H, Liu J, Zhou C, Gao N, Rao Z, Li H, Hu X, Li C, Yao X, Shen X, Sun Y, Wei Y, Liu F, Ying W, Zhang J, Tang C, Zhang X, Xu H, Shi L, Cheng L, Huang P, Yang H. Nat Neurosci. 2018 Jan 15. pii: 10.1038/s41593-017-0060-6. doi: 10.1038/s41593-017-0060-6. PubMed

Associated Plasmids
Human Pancreatic Tumor Organoids Reveal Loss of Stem Cell Niche Factor Dependence during Disease Progression. Seino T, Kawasaki S, Shimokawa M, Tamagawa H, Toshimitsu K, Fujii M, Ohta Y, Matano M, Nanki K, Kawasaki K, Takahashi S, Sugimoto S, Iwasaki E, Takagi J, Itoi T, Kitago M, Kitagawa Y, Kanai T, Sato T. Cell Stem Cell. 2018 Mar 1;22(3):454-467.e6. doi: 10.1016/j.stem.2017.12.009. Epub 2018 Jan 11. PubMed
Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells. Abdelfattah NS, Mullally A. J Vis Exp. 2018 Jan 5;(131). doi: 10.3791/56726. PubMed
Inhibition of Dpp8/9 Activates the Nlrp1b Inflammasome. Okondo MC, Rao SD, Taabazuing CY, Chui AJ, Poplawski SE, Johnson DC, Bachovchin DA. Cell Chem Biol. 2018 Mar 15;25(3):262-267.e5. doi: 10.1016/j.chembiol.2017.12.013. Epub 2018 Jan 27. PubMed
Genome-wide tracking of dCas9-methyltransferase footprints. Galonska C, Charlton J, Mattei AL, Donaghey J, Clement K, Gu H, Mohammad AW, Stamenova EK, Cacchiarelli D, Klages S, Timmermann B, Cantz T, Scholer HR, Gnirke A, Ziller MJ, Meissner A. Nat Commun. 2018 Feb 9;9(1):597. doi: 10.1038/s41467-017-02708-5. PubMed
Next generation high throughput DNA damage detection platform for genotoxic compound screening. Sykora P, Witt KL, Revanna P, Smith-Roe SL, Dismukes J, Lloyd DG, Engelward BP, Sobol RW. Sci Rep. 2018 Feb 9;8(1):2771. doi: 10.1038/s41598-018-20995-w. PubMed
Combinatorial CRISPR-Cas9 Metabolic Screens Reveal Critical Redox Control Points Dependent on the KEAP1-NRF2 Regulatory Axis. Zhao D, Badur MG, Luebeck J, Magana JH, Birmingham A, Sasik R, Ahn CS, Ideker T, Metallo CM, Mali P. Mol Cell. 2018 Feb 15;69(4):699-708.e7. doi: 10.1016/j.molcel.2018.01.017. PubMed
On the design of CRISPR-based single-cell molecular screens. Hill AJ, McFaline-Figueroa JL, Starita LM, Gasperini MJ, Matreyek KA, Packer J, Jackson D, Shendure J, Trapnell C. Nat Methods. 2018 Feb 19. pii: nmeth.4604. doi: 10.1038/nmeth.4604. PubMed

Associated Plasmids
Genome-wide CRISPR-Cas9 Screen Identifies Leukemia-Specific Dependence on a Pre-mRNA Metabolic Pathway Regulated by DCPS. Yamauchi T, Masuda T, Canver MC, Seiler M, Semba Y, Shboul M, Al-Raqad M, Maeda M, Schoonenberg VAC, Cole MA, Macias-Trevino C, Ishikawa Y, Yao Q, Nakano M, Arai F, Orkin SH, Reversade B, Buonamici S, Pinello L, Akashi K, Bauer DE, Maeda T. Cancer Cell. 2018 Mar 12;33(3):386-400.e5. doi: 10.1016/j.ccell.2018.01.012. Epub 2018 Feb 22. PubMed
Inhibition of TRPC1-Dependent Store-Operated Calcium Entry Improves Synaptic Stability and Motor Performance in a Mouse Model of Huntington's Disease. Wu J, Ryskamp D, Birnbaumer L, Bezprozvanny I. J Huntingtons Dis. 2018;7(1):35-50. doi: 10.3233/JHD-170266. PubMed
Human CHD1 is required for early DNA-damage signaling and is uniquely regulated by its N terminus. Zhou J, Li J, Serafim RB, Ketchum S, Ferreira CG, Liu JC, Coe KA, Price BD, Yusufzai T. Nucleic Acids Res. 2018 May 4;46(8):3891-3905. doi: 10.1093/nar/gky128. PubMed
GATA3 zinc finger 2 mutations reprogram the breast cancer transcriptional network. Takaku M, Grimm SA, Roberts JD, Chrysovergis K, Bennett BD, Myers P, Perera L, Tucker CJ, Perou CM, Wade PA. Nat Commun. 2018 Mar 13;9(1):1059. doi: 10.1038/s41467-018-03478-4. PubMed
PI4KIIalpha regulates insulin secretion and glucose homeostasis via a PKD-dependent pathway. Zhang L, Li J, Zhang P, Gao Z, Zhao Y, Qiao X, Chen C. Biophys Rep. 2018;4(1):25-38. doi: 10.1007/s41048-018-0049-z. Epub 2018 Mar 7. PubMed
Direct Promoter Repression by BCL11A Controls the Fetal to Adult Hemoglobin Switch. Liu N, Hargreaves VV, Zhu Q, Kurland JV, Hong J, Kim W, Sher F, Macias-Trevino C, Rogers JM, Kurita R, Nakamura Y, Yuan GC, Bauer DE, Xu J, Bulyk ML, Orkin SH. Cell. 2018 Apr 5;173(2):430-442.e17. doi: 10.1016/j.cell.2018.03.016. Epub 2018 Mar 29. PubMed
Genome-wide CRISPR/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication. Han J, Perez JT, Chen C, Li Y, Benitez A, Kandasamy M, Lee Y, Andrade J, tenOever B, Manicassamy B. Cell Rep. 2018 Apr 10;23(2):596-607. doi: 10.1016/j.celrep.2018.03.045. PubMed
Reciprocal Network between Cancer Stem-Like Cells and Macrophages Facilitates the Progression and Androgen Deprivation Therapy Resistance of Prostate Cancer. Huang H, Wang C, Liu F, Li HZ, Peng G, Gao X, Dong KQ, Wang HR, Kong DP, Qu M, Dai LH, Wang KJ, Zhou Z, Yang J, Yang ZY, Cheng YQ, Tian QQ, Liu D, Xu CL, Xu DF, Cui XG, Sun YH. Clin Cancer Res. 2018 Sep 15;24(18):4612-4626. doi: 10.1158/1078-0432.CCR-18-0461. Epub 2018 Apr 24. PubMed
PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression. Zheng YZ, Xue MZ, Shen HJ, Li XG, Ma D, Gong Y, Liu YR, Qiao F, Xie HY, Lian B, Sun WL, Zhao HY, Yao L, Zuo WJ, Li DQ, Wang P, Hu X, Shao ZM. Cancer Res. 2018 Jun 15;78(12):3190-3206. doi: 10.1158/0008-5472.CAN-17-3514. Epub 2018 Apr 26. PubMed
PDLIM7 and CDH18 regulate the turnover of MDM2 during CDK4/6 inhibitor therapy-induced senescence. Klein ME, Dickson MA, Antonescu C, Qin LX, Dooley SJ, Barlas A, Manova K, Schwartz GK, Crago AM, Singer S, Koff A, Tap WD. Oncogene. 2018 Sep;37(37):5066-5078. doi: 10.1038/s41388-018-0332-y. Epub 2018 May 23. PubMed
CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response. Haapaniemi E, Botla S, Persson J, Schmierer B, Taipale J. Nat Med. 2018 Jul;24(7):927-930. doi: 10.1038/s41591-018-0049-z. Epub 2018 Jun 11. PubMed
iTAP, a novel iRhom interactor, controls TNF secretion by policing the stability of iRhom/TACE. Oikonomidi I, Burbridge E, Cavadas M, Sullivan G, Collis B, Naegele H, Clancy D, Brezinova J, Hu T, Bileck A, Gerner C, Bolado A, von Kriegsheim A, Martin SJ, Steinberg F, Strisovsky K, Adrain C. Elife. 2018 Jun 13;7. pii: 35032. doi: 10.7554/eLife.35032. PubMed
Producing anti-inflammatory macrophages by nanoparticle-triggered clustering of mannose receptors. Gan J, Dou Y, Li Y, Wang Z, Wang L, Liu S, Li Q, Yu H, Liu C, Han C, Huang Z, Zhang J, Wang C, Dong L. Biomaterials. 2018 Sep;178:95-108. doi: 10.1016/j.biomaterials.2018.06.015. Epub 2018 Jun 14. PubMed
Identification, characterization and application of a new peptide against anterior gradient homolog 2 (AGR2). Garri C, Howell S, Tiemann K, Tiffany A, Jalali-Yazdi F, Alba MM, Katz JE, Takahashi TT, Landgraf R, Gross ME, Roberts RW, Kani K. Oncotarget. 2018 Jun 8;9(44):27363-27379. doi: 10.18632/oncotarget.25221. eCollection 2018 Jun 8. PubMed
CK1alpha and IRF4 are essential and independent effectors of immunomodulatory drugs in primary effusion lymphoma. Patil A, Manzano M, Gottwein E. Blood. 2018 Aug 9;132(6):577-586. doi: 10.1182/blood-2018-01-828418. Epub 2018 Jun 28. PubMed

Associated Plasmids
Expression of Long Noncoding RNA YIYA Promotes Glycolysis in Breast Cancer. Xing Z, Zhang Y, Liang K, Yan L, Xiang Y, Li C, Hu Q, Jin F, Putluri V, Putluri N, Coarfa C, Sreekumar A, Park PK, Nguyen TK, Wang S, Zhou J, Zhou Y, Marks JR, Hawke DH, Hung MC, Yang L, Han L, Ying H, Lin C. Cancer Res. 2018 Aug 15;78(16):4524-4532. doi: 10.1158/0008-5472.CAN-17-0385. Epub 2018 Jul 2. PubMed
DPP8/DPP9 inhibitor-induced pyroptosis for treatment of acute myeloid leukemia. Johnson DC, Taabazuing CY, Okondo MC, Chui AJ, Rao SD, Brown FC, Reed C, Peguero E, de Stanchina E, Kentsis A, Bachovchin DA. Nat Med. 2018 Aug;24(8):1151-1156. doi: 10.1038/s41591-018-0082-y. Epub 2018 Jul 2. PubMed
CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. Zimmermann M, Murina O, Reijns MAM, Agathanggelou A, Challis R, Tarnauskaite Z, Muir M, Fluteau A, Aregger M, McEwan A, Yuan W, Clarke M, Lambros MB, Paneesha S, Moss P, Chandrashekhar M, Angers S, Moffat J, Brunton VG, Hart T, de Bono J, Stankovic T, Jackson AP, Durocher D. Nature. 2018 Jul;559(7713):285-289. doi: 10.1038/s41586-018-0291-z. Epub 2018 Jul 4. PubMed
Mutant ASXL1 cooperates with BAP1 to promote myeloid leukaemogenesis. Asada S, Goyama S, Inoue D, Shikata S, Takeda R, Fukushima T, Yonezawa T, Fujino T, Hayashi Y, Kawabata KC, Fukuyama T, Tanaka Y, Yokoyama A, Yamazaki S, Kozuka-Hata H, Oyama M, Kojima S, Kawazu M, Mano H, Kitamura T. Nat Commun. 2018 Jul 16;9(1):2733. doi: 10.1038/s41467-018-05085-9. PubMed
The shieldin complex mediates 53BP1-dependent DNA repair. Noordermeer SM, Adam S, Setiaputra D, Barazas M, Pettitt SJ, Ling AK, Olivieri M, Alvarez-Quilon A, Moatti N, Zimmermann M, Annunziato S, Krastev DB, Song F, Brandsma I, Frankum J, Brough R, Sherker A, Landry S, Szilard RK, Munro MM, McEwan A, Goullet de Rugy T, Lin ZY, Hart T, Moffat J, Gingras AC, Martin A, van Attikum H, Jonkers J, Lord CJ, Rottenberg S, Durocher D. Nature. 2018 Aug;560(7716):117-121. doi: 10.1038/s41586-018-0340-7. Epub 2018 Jul 18. PubMed

Associated Plasmids
Risk SNP-Mediated Promoter-Enhancer Switching Drives Prostate Cancer through lncRNA PCAT19. Hua JT, Ahmed M, Guo H, Zhang Y, Chen S, Soares F, Lu J, Zhou S, Wang M, Li H, Larson NB, McDonnell SK, Patel PS, Liang Y, Yao CQ, van der Kwast T, Lupien M, Feng FY, Zoubeidi A, Tsao MS, Thibodeau SN, Boutros PC, He HH. Cell. 2018 Jul 26;174(3):564-575.e18. doi: 10.1016/j.cell.2018.06.014. Epub 2018 Jul 19. PubMed
A novel enhancer regulates MGMT expression and promotes temozolomide resistance in glioblastoma. Chen X, Zhang M, Gan H, Wang H, Lee JH, Fang D, Kitange GJ, He L, Hu Z, Parney IF, Meyer FB, Giannini C, Sarkaria JN, Zhang Z. Nat Commun. 2018 Jul 27;9(1):2949. doi: 10.1038/s41467-018-05373-4. PubMed
Hippo signaling dysfunction induces cancer cell addiction to YAP. Han H, Yang B, Nakaoka HJ, Yang J, Zhao Y, Le Nguyen K, Bishara AT, Mandalia TK, Wang W. Oncogene. 2018 Dec;37(50):6414-6424. doi: 10.1038/s41388-018-0419-5. Epub 2018 Aug 1. PubMed
Reconstruction of the cell entry pathway of an extinct virus. Robinson-McCarthy LR, McCarthy KR, Raaben M, Piccinotti S, Nieuwenhuis J, Stubbs SH, Bakkers MJG, Whelan SPJ. PLoS Pathog. 2018 Aug 6;14(8):e1007123. doi: 10.1371/journal.ppat.1007123. eCollection 2018 Aug. PubMed
Nrf2 negatively regulates STING indicating a link between antiviral sensing and metabolic reprogramming. Olagnier D, Brandtoft AM, Gunderstofte C, Villadsen NL, Krapp C, Thielke AL, Laustsen A, Peri S, Hansen AL, Bonefeld L, Thyrsted J, Bruun V, Iversen MB, Lin L, Artegoitia VM, Su C, Yang L, Lin R, Balachandran S, Luo Y, Nyegaard M, Marrero B, Goldbach-Mansky R, Motwani M, Ryan DG, Fitzgerald KA, O'Neill LA, Hollensen AK, Damgaard CK, de Paoli FV, Bertram HC, Jakobsen MR, Poulsen TB, Holm CK. Nat Commun. 2018 Aug 29;9(1):3506. doi: 10.1038/s41467-018-05861-7. PubMed
Wnt Effector TCF4 Is Dispensable for Wnt Signaling in Human Cancer Cells. Hrckulak D, Janeckova L, Lanikova L, Kriz V, Horazna M, Babosova O, Vojtechova M, Galuskova K, Sloncova E, Korinek V. Genes (Basel). 2018 Sep 1;9(9). pii: genes9090439. doi: 10.3390/genes9090439. PubMed
PLK1 targets CtIP to promote microhomology-mediated end joining. Wang H, Qiu Z, Liu B, Wu Y, Ren J, Liu Y, Zhao Y, Wang Y, Hao S, Li Z, Peng B, Xu X. Nucleic Acids Res. 2018 Nov 16;46(20):10724-10739. doi: 10.1093/nar/gky810. PubMed
Transaminase Inhibition by 2-Hydroxyglutarate Impairs Glutamate Biosynthesis and Redox Homeostasis in Glioma. McBrayer SK, Mayers JR, DiNatale GJ, Shi DD, Khanal J, Chakraborty AA, Sarosiek KA, Briggs KJ, Robbins AK, Sewastianik T, Shareef SJ, Olenchock BA, Parker SJ, Tateishi K, Spinelli JB, Islam M, Haigis MC, Looper RE, Ligon KL, Bernstein BE, Carrasco RD, Cahill DP, Asara JM, Metallo CM, Yennawar NH, Vander Heiden MG, Kaelin WG Jr. Cell. 2018 Sep 20;175(1):101-116.e25. doi: 10.1016/j.cell.2018.08.038. Epub 2018 Sep 13. PubMed
Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking. Fauster A, Rebsamen M, Willmann KL, Cesar-Razquin A, Girardi E, Bigenzahn JW, Schischlik F, Scorzoni S, Bruckner M, Konecka J, Hormann K, Heinz LX, Boztug K, Superti-Furga G. Cell Death Differ. 2018 Sep 20. pii: 10.1038/s41418-018-0192-6. doi: 10.1038/s41418-018-0192-6. PubMed
Changes in mRNA abundance drive shuttling of RNA binding proteins, linking cytoplasmic RNA degradation to transcription. Gilbertson S, Federspiel JD, Hartenian E, Cristea IM, Glaunsinger B. Elife. 2018 Oct 3;7. pii: 37663. doi: 10.7554/eLife.37663. PubMed

Associated Plasmids
Compound C inhibits nonsense-mediated RNA decay independently of AMPK. Cheruiyot A, Li S, Nickless A, Roth R, Fitzpatrick JAJ, You Z. PLoS One. 2018 Oct 5;13(10):e0204978. doi: 10.1371/journal.pone.0204978. eCollection 2018. PubMed
Human DPP9 represses NLRP1 inflammasome and protects against autoinflammatory diseases via both peptidase activity and FIIND domain binding. Zhong FL, Robinson K, Teo DET, Tan KY, Lim C, Harapas CR, Yu CH, Xie WH, Sobota RM, Au VB, Hopkins R, D'Osualdo A, Reed JC, Connolly JE, Masters SL, Reversade B. J Biol Chem. 2018 Dec 7;293(49):18864-18878. doi: 10.1074/jbc.RA118.004350. Epub 2018 Oct 5. PubMed
Regulation of the Hippo Pathway by Phosphatidic Acid-Mediated Lipid-Protein Interaction. Han H, Qi R, Zhou JJ, Ta AP, Yang B, Nakaoka HJ, Seo G, Guan KL, Luo R, Wang W. Mol Cell. 2018 Oct 18;72(2):328-340.e8. doi: 10.1016/j.molcel.2018.08.038. Epub 2018 Oct 4. PubMed
Trichomonas vaginalis Induces NLRP3 Inflammasome Activation and Pyroptotic Cell Death in Human Macrophages. Riestra AM, Valderrama JA, Patras KA, Booth SD, Quek XY, Tsai CM, Nizet V. J Innate Immun. 2019;11(1):86-98. doi: 10.1159/000493585. Epub 2018 Nov 2. PubMed
Inhibition of Thioredoxin/Thioredoxin Reductase Induces Synthetic Lethality in Lung Cancers with Compromised Glutathione Homeostasis. Yan X, Zhang X, Wang L, Zhang R, Pu X, Wu S, Li L, Tong P, Wang J, Meng QH, Jensen VB, Girard L, Minna JD, Roth JA, Swisher SG, Heymach JV, Fang B. Cancer Res. 2019 Jan 1;79(1):125-132. doi: 10.1158/0008-5472.CAN-18-1938. Epub 2018 Nov 6. PubMed
PRC2 loss induces chemoresistance by repressing apoptosis in T cell acute lymphoblastic leukemia. Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB, Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, Gutierrez A. J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570. PubMed

Associated Plasmids
DEP domain containing 1 predicts prognosis of hepatocellular carcinoma patients and regulates tumor proliferation and metastasis. Qu D, Cui F, Lu D, Yang Y, Xu Y. Cancer Sci. 2019 Jan;110(1):157-165. doi: 10.1111/cas.13867. Epub 2018 Dec 13. PubMed
Fibroblast Growth Factor 2 lethally sensitizes cancer cells to stress-targeted therapeutic inhibitors. Dias MH, Fonseca CS, Zeidler JD, Albuquerque LL, da Silva MS, Cararo-Lopes E, Reis MS, Noel V, Dos Santos EO, Prior IA, Armelin HA. Mol Oncol. 2019 Feb;13(2):290-306. doi: 10.1002/1878-0261.12402. Epub 2018 Dec 3. PubMed
Sigma-2 Receptor/TMEM97 and PGRMC-1 Increase the Rate of Internalization of LDL by LDL Receptor through the Formation of a Ternary Complex. Riad A, Zeng C, Weng CC, Winters H, Xu K, Makvandi M, Metz T, Carlin S, Mach RH. Sci Rep. 2018 Nov 15;8(1):16845. doi: 10.1038/s41598-018-35430-3. PubMed
Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function. Shifrut E, Carnevale J, Tobin V, Roth TL, Woo JM, Bui CT, Li PJ, Diolaiti ME, Ashworth A, Marson A. Cell. 2018 Dec 13;175(7):1958-1971.e15. doi: 10.1016/j.cell.2018.10.024. Epub 2018 Nov 15. PubMed
DYNLL1 binds to MRE11 to limit DNA end resection in BRCA1-deficient cells. He YJ, Meghani K, Caron MC, Yang C, Ronato DA, Bian J, Sharma A, Moore J, Niraj J, Detappe A, Doench JG, Legube G, Root DE, D'Andrea AD, Drane P, De S, Konstantinopoulos PA, Masson JY, Chowdhury D. Nature. 2018 Nov;563(7732):522-526. doi: 10.1038/s41586-018-0670-5. Epub 2018 Oct 31. PubMed
Efferocytosis induces a novel SLC program to promote glucose uptake and lactate release. Morioka S, Perry JSA, Raymond MH, Medina CB, Zhu Y, Zhao L, Serbulea V, Onengut-Gumuscu S, Leitinger N, Kucenas S, Rathmell JC, Makowski L, Ravichandran KS. Nature. 2018 Nov;563(7733):714-718. doi: 10.1038/s41586-018-0735-5. Epub 2018 Nov 21. PubMed
Genome-wide CRISPR screens for Shiga toxins and ricin reveal Golgi proteins critical for glycosylation. Tian S, Muneeruddin K, Choi MY, Tao L, Bhuiyan RH, Ohmi Y, Furukawa K, Furukawa K, Boland S, Shaffer SA, Adam RM, Dong M. PLoS Biol. 2018 Nov 27;16(11):e2006951. doi: 10.1371/journal.pbio.2006951. eCollection 2018 Nov. PubMed
High-performance gene expression and knockout tools using sleeping beauty transposon system. Hu K, Li Y, Wu W, Chen H, Chen Z, Zhang Y, Guo Y, Dong Y. Mob DNA. 2018 Nov 26;9:33. doi: 10.1186/s13100-018-0139-y. eCollection 2018. PubMed
Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities. Sanson KR, Hanna RE, Hegde M, Donovan KF, Strand C, Sullender ME, Vaimberg EW, Goodale A, Root DE, Piccioni F, Doench JG. Nat Commun. 2018 Dec 21;9(1):5416. doi: 10.1038/s41467-018-07901-8. PubMed

Associated Plasmids
An Epithelial-to-Mesenchymal Transcriptional Switch Triggers Evolution of Pulmonary Sarcomatoid Carcinoma (PSC) and Identifies Dasatinib as New Therapeutic Option. Manzotti G, Torricelli F, Benedetta D, Lococo F, Sancisi V, Rossi G, Piana S, Ciarrocchi A. Clin Cancer Res. 2019 Apr 1;25(7):2348-2360. doi: 10.1158/1078-0432.CCR-18-2364. Epub 2018 Dec 26. PubMed
Knockout of MTF1 Inhibits the Epithelial to Mesenchymal Transition in Ovarian Cancer Cells. Ji L, Zhao G, Zhang P, Huo W, Dong P, Watari H, Jia L, Pfeffer LM, Yue J, Zheng J. J Cancer. 2018 Nov 11;9(24):4578-4585. doi: 10.7150/jca.28040. eCollection 2018. PubMed
The Ca(2+) sensor STIM1 regulates the type I interferon response by retaining the signaling adaptor STING at the endoplasmic reticulum. Srikanth S, Woo JS, Wu B, El-Sherbiny YM, Leung J, Chupradit K, Rice L, Seo GJ, Calmettes G, Ramakrishna C, Cantin E, An DS, Sun R, Wu TT, Jung JU, Savic S, Gwack Y. Nat Immunol. 2019 Feb;20(2):152-162. doi: 10.1038/s41590-018-0287-8. Epub 2019 Jan 14. PubMed
Long non-coding RNA PSMB8-AS1 regulates influenza virus replication. More S, Zhu Z, Lin K, Huang C, Pushparaj S, Liang Y, Sathiaseelan R, Yang X, Liu L. RNA Biol. 2019 Mar;16(3):340-353. doi: 10.1080/15476286.2019.1572448. Epub 2019 Jan 28. PubMed
LncRNA MIR22HG inhibits growth, migration and invasion through regulating the miR-10a-5p/NCOR2 axis in hepatocellular carcinoma cells. Wu Y, Zhou Y, Huan L, Xu L, Shen M, Huang S, Liang L. Cancer Sci. 2019 Mar;110(3):973-984. doi: 10.1111/cas.13950. Epub 2019 Feb 23. PubMed
Overexpression of NEUROG2 and NEUROG1 in human embryonic stem cells produces a network of excitatory and inhibitory neurons. Lu C, Shi X, Allen A, Baez-Nieto D, Nikish A, Sanjana NE, Pan JQ. FASEB J. 2019 Apr;33(4):5287-5299. doi: 10.1096/fj.201801110RR. Epub 2019 Jan 30. PubMed
De Novo PITX1 Expression Controls Bi-Stable Transcriptional Circuits to Govern Self-Renewal and Differentiation in Squamous Cell Carcinoma. Sastre-Perona A, Hoang-Phou S, Leitner MC, Okuniewska M, Meehan S, Schober M. Cell Stem Cell. 2019 Mar 7;24(3):390-404.e8. doi: 10.1016/j.stem.2019.01.003. Epub 2019 Jan 31. PubMed
Identification of Novel Regulatory Genes in APAP Induced Hepatocyte Toxicity by a Genome-Wide CRISPR-Cas9 Screen. Shortt K, Heruth DP, Zhang N, Wu W, Singh S, Li DY, Zhang LQ, Wyckoff GJ, Qi LS, Friesen CA, Ye SQ. Sci Rep. 2019 Feb 4;9(1):1396. doi: 10.1038/s41598-018-37940-6. PubMed
Neutrophils escort circulating tumour cells to enable cell cycle progression. Szczerba BM, Castro-Giner F, Vetter M, Krol I, Gkountela S, Landin J, Scheidmann MC, Donato C, Scherrer R, Singer J, Beisel C, Kurzeder C, Heinzelmann-Schwarz V, Rochlitz C, Weber WP, Beerenwinkel N, Aceto N. Nature. 2019 Feb;566(7745):553-557. doi: 10.1038/s41586-019-0915-y. Epub 2019 Feb 6. PubMed
LncEGFL7OS regulates human angiogenesis by interacting with MAX at the EGFL7/miR-126 locus. Zhou Q, Yu B, Anderson C, Huang ZP, Hanus J, Zhang W, Han Y, Bhattacharjee PS, Srinivasan S, Zhang K, Wang DZ, Wang S. Elife. 2019 Feb 11;8. pii: 40470. doi: 10.7554/eLife.40470. PubMed
Ephrin Receptor A4 is a New Kaposi's Sarcoma-Associated Herpesvirus Virus Entry Receptor. Chen J, Zhang X, Schaller S, Jardetzky TS, Longnecker R. MBio. 2019 Feb 19;10(1). pii: mBio.02892-18. doi: 10.1128/mBio.02892-18. PubMed
High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy. Bowler M, Kong D, Sun S, Nanjundappa R, Evans L, Farmer V, Holland A, Mahjoub MR, Sui H, Loncarek J. Nat Commun. 2019 Mar 1;10(1):993. doi: 10.1038/s41467-018-08216-4. PubMed
Neddylation Inactivation Facilitates FOXO3a Nuclear Export to Suppress Estrogen Receptor Transcription and Improve Fulvestrant Sensitivity. Jia X, Li C, Li L, Liu X, Zhou L, Zhang W, Ni S, Lu Y, Chen L, Jeong LS, Yu J, Zhang Y, Zhang J, He S, Hu X, Sun H, Yu K, Liu G, Zhao H, Zhang Y, Jia L, Shao ZM. Clin Cancer Res. 2019 Jun 15;25(12):3658-3672. doi: 10.1158/1078-0432.CCR-18-2434. Epub 2019 Mar 4. PubMed
Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome. Wegner M, Diehl V, Bittl V, de Bruyn R, Wiechmann S, Matthess Y, Hebel M, Hayes MG, Schaubeck S, Benner C, Heinz S, Bremm A, Dikic I, Ernst A, Kaulich M. Elife. 2019 Mar 6;8. pii: 42549. doi: 10.7554/eLife.42549. PubMed
The Impact of HIV-1 Genetic Diversity on CRISPR-Cas9 Antiviral Activity and Viral Escape. Darcis G, Binda CS, Klaver B, Herrera-Carrillo E, Berkhout B, Das AT. Viruses. 2019 Mar 13;11(3). pii: v11030255. doi: 10.3390/v11030255. PubMed
Lineage tracing using a Cas9-deaminase barcoding system targeting endogenous L1 elements. Hwang B, Lee W, Yum SY, Jeon Y, Cho N, Jang G, Bang D. Nat Commun. 2019 Mar 15;10(1):1234. doi: 10.1038/s41467-019-09203-z. PubMed
AKT2 phosphorylation of hexokinase 2 at T473 promotes tumorigenesis and metastasis in colon cancer cells via NF-kappaB, HIF1alpha, MMP2, and MMP9 upregulation. Li H, Lu S, Chen Y, Zheng L, Chen L, Ding H, Ding J, Lou D, Liu F, Zheng B. Cell Signal. 2019 Jun;58:99-110. doi: 10.1016/j.cellsig.2019.03.011. Epub 2019 Mar 12. PubMed
RNAi-based small molecule repositioning reveals clinically approved urea-based kinase inhibitors as broadly active antivirals. Lesch M, Luckner M, Meyer M, Weege F, Gravenstein I, Raftery M, Sieben C, Martin-Sancho L, Imai-Matsushima A, Welke RW, Frise R, Barclay W, Schonrich G, Herrmann A, Meyer TF, Karlas A. PLoS Pathog. 2019 Mar 18;15(3):e1007601. doi: 10.1371/journal.ppat.1007601. eCollection 2019 Mar. PubMed
Elucidating the mechanism of action of domatinostat (4SC-202) in cutaneous T cell lymphoma cells. Wobser M, Weber A, Glunz A, Tauch S, Seitz K, Butelmann T, Hesbacher S, Goebeler M, Bartz R, Kohlhof H, Schrama D, Houben R. J Hematol Oncol. 2019 Mar 18;12(1):30. doi: 10.1186/s13045-019-0719-4. PubMed
LION: a simple and rapid method to achieve CRISPR gene editing. Xiang X, Luo L, Nodzynski M, Li C, Han P, Dou H, Petersen TS, Liang X, Pan X, Qu K, Yang L, Dang Y, Liu X, Bolund L, Zhang X, Tong G, Xing Y, Luo Y, Lin L. Cell Mol Life Sci. 2019 Jul;76(13):2633-2645. doi: 10.1007/s00018-019-03064-x. Epub 2019 Mar 18. PubMed
Production and Validation of Lentiviral Vectors for CRISPR/Cas9 Delivery. Ryo LB, Thomsen EA, Mikkelsen JG. Methods Mol Biol. 2019;1961:93-109. doi: 10.1007/978-1-4939-9170-9_7. PubMed
PRMT1 loss sensitizes cells to PRMT5 inhibition. Gao G, Zhang L, Villarreal OD, He W, Su D, Bedford E, Moh P, Shen J, Shi X, Bedford MT, Xu H. Nucleic Acids Res. 2019 Jun 4;47(10):5038-5048. doi: 10.1093/nar/gkz200. PubMed
The long noncoding RNA ROCKI regulates inflammatory gene expression. Zhang Q, Chao TC, Patil VS, Qin Y, Tiwari SK, Chiou J, Dobin A, Tsai CM, Li Z, Dang J, Gupta S, Urdahl K, Nizet V, Gingeras TR, Gaulton KJ, Rana TM. EMBO J. 2019 Apr 15;38(8). pii: embj.2018100041. doi: 10.15252/embj.2018100041. Epub 2019 Mar 27. PubMed
Targeting Super-Enhancer-Driven Oncogenic Transcription by CDK7 Inhibition in Anaplastic Thyroid Carcinoma. Cao X, Dang L, Zheng X, Lu Y, Lu Y, Ji R, Zhang T, Ruan X, Zhi J, Hou X, Yi X, Li MJ, Gu T, Gao M, Zhang L, Chen Y. Thyroid. 2019 Jun;29(6):809-823. doi: 10.1089/thy.2018.0550. Epub 2019 May 3. PubMed
CRISPR screening using an expanded toolkit of autophagy reporters identifies TMEM41B as a novel autophagy factor. Shoemaker CJ, Huang TQ, Weir NR, Polyakov NJ, Schultz SW, Denic V. PLoS Biol. 2019 Apr 1;17(4):e2007044. doi: 10.1371/journal.pbio.2007044. eCollection 2019 Apr. PubMed
Competitive endogenous RNA is an intrinsic component of EMT regulatory circuits and modulates EMT. Liu Y, Xue M, Du S, Feng W, Zhang K, Zhang L, Liu H, Jia G, Wu L, Hu X, Chen L, Wang P. Nat Commun. 2019 Apr 9;10(1):1637. doi: 10.1038/s41467-019-09649-1. PubMed
Metformin inhibits IL-6 signaling by decreasing IL-6R expression on multiple myeloma cells. Mishra AK, Dingli D. Leukemia. 2019 Apr 15. pii: 10.1038/s41375-019-0470-4. doi: 10.1038/s41375-019-0470-4. PubMed
CCL5 promotes breast cancer recurrence through macrophage recruitment in residual tumors. Walens A, DiMarco AV, Lupo R, Kroger BR, Damrauer JS, Alvarez JV. Elife. 2019 Apr 16;8. pii: 43653. doi: 10.7554/eLife.43653. PubMed
Synthetic Lethality of Wnt Pathway Activation and Asparaginase in Drug-Resistant Acute Leukemias. Hinze L, Pfirrmann M, Karim S, Degar J, McGuckin C, Vinjamur D, Sacher J, Stevenson KE, Neuberg DS, Orellana E, Stanulla M, Gregory RI, Bauer DE, Wagner FF, Stegmaier K, Gutierrez A. Cancer Cell. 2019 Apr 15;35(4):664-676.e7. doi: 10.1016/j.ccell.2019.03.004. PubMed
TAF5L and TAF6L Maintain Self-Renewal of Embryonic Stem Cells via the MYC Regulatory Network. Seruggia D, Oti M, Tripathi P, Canver MC, LeBlanc L, Di Giammartino DC, Bullen MJ, Nefzger CM, Sun YBY, Farouni R, Polo JM, Pinello L, Apostolou E, Kim J, Orkin SH, Das PP. Mol Cell. 2019 Jun 20;74(6):1148-1163.e7. doi: 10.1016/j.molcel.2019.03.025. Epub 2019 Apr 17. PubMed
NAD metabolic dependency in cancer is shaped by gene amplification and enhancer remodelling. Chowdhry S, Zanca C, Rajkumar U, Koga T, Diao Y, Raviram R, Liu F, Turner K, Yang H, Brunk E, Bi J, Furnari F, Bafna V, Ren B, Mischel PS. Nature. 2019 May;569(7757):570-575. doi: 10.1038/s41586-019-1150-2. Epub 2019 Apr 24. PubMed
BIRC5 is a target for molecular imaging and detection of human pancreatic cancer. Liu SH, Hong Y, Markowiak S, Sanchez R, Creeden J, Nemunaitis J, Kalinoski A, Willey J, Erhardt P, Lee J, van Dam M, Brunicardi FC. Cancer Lett. 2019 Aug 10;457:10-19. doi: 10.1016/j.canlet.2019.04.036. Epub 2019 May 3. PubMed
PES1 is a critical component of telomerase assembly and regulates cellular senescence. Cheng L, Yuan B, Ying S, Niu C, Mai H, Guan X, Yang X, Teng Y, Lin J, Huang J, Jin R, Wu J, Liu B, Chang S, Wang E, Zhang C, Hou N, Cheng X, Xu D, Yang X, Gao S, Ye Q. Sci Adv. 2019 May 15;5(5):eaav1090. doi: 10.1126/sciadv.aav1090. eCollection 2019 May. PubMed
Genome-scale screens identify JNK-JUN signaling as a barrier for pluripotency exit and endoderm differentiation. Li QV, Dixon G, Verma N, Rosen BP, Gordillo M, Luo R, Xu C, Wang Q, Soh CL, Yang D, Crespo M, Shukla A, Xiang Q, Dundar F, Zumbo P, Witkin M, Koche R, Betel D, Chen S, Massague J, Garippa R, Evans T, Beer MA, Huangfu D. Nat Genet. 2019 Jun;51(6):999-1010. doi: 10.1038/s41588-019-0408-9. Epub 2019 May 20. PubMed
Comprehensive substrate specificity profiling of the human Nek kinome reveals unexpected signaling outputs. van de Kooij B, Creixell P, van Vlimmeren A, Joughin BA, Miller CJ, Haider N, Simpson CD, Linding R, Stambolic V, Turk BE, Yaffe MB. Elife. 2019 May 24;8. pii: 44635. doi: 10.7554/eLife.44635. PubMed
Mutant-selective topologic conversion facilitates selective degradation of a pathogenic prion isoform. Lee Y, Eum H, Lee D, Lee S, Song Y, Kang SW. Cell Death Differ. 2019 May 24. pii: 10.1038/s41418-019-0354-1. doi: 10.1038/s41418-019-0354-1. PubMed
Identification of Renal Long Non-coding RNA RP11-2B6.2 as a Positive Regulator of Type I Interferon Signaling Pathway in Lupus Nephritis. Liao Z, Ye Z, Xue Z, Wu L, Ouyang Y, Yao C, Cui C, Xu N, Ma J, Hou G, Wang J, Meng Y, Yin Z, Liu Y, Qian J, Zhang C, Ding H, Guo Q, Qu B, Shen N. Front Immunol. 2019 May 3;10:975. doi: 10.3389/fimmu.2019.00975. eCollection 2019. PubMed
Generation of an immortalized erythroid progenitor cell line from peripheral blood: A model system for the functional analysis of Plasmodium spp. invasion. Scully EJ, Shabani E, Rangel GW, Gruring C, Kanjee U, Clark MA, Chaand M, Kurita R, Nakamura Y, Ferreira MU, Duraisingh MT. Am J Hematol. 2019 May 31. doi: 10.1002/ajh.25543. PubMed
Opposing effects of acute versus chronic inhibition of p53 on decitabine's efficacy in myeloid neoplasms. Tamura M, Yonezawa T, Liu X, Asada S, Hayashi Y, Fukuyama T, Tanaka Y, Kitamura T, Goyama S. Sci Rep. 2019 Jun 3;9(1):8171. doi: 10.1038/s41598-019-44496-6. PubMed
Massively parallel profiling and predictive modeling of the outcomes of CRISPR/Cas9-mediated double-strand break repair. Chen W, McKenna A, Schreiber J, Haeussler M, Yin Y, Agarwal V, Noble WS, Shendure J. Nucleic Acids Res. 2019 Sep 5;47(15):7989-8003. doi: 10.1093/nar/gkz487. PubMed
Overexpression of the Ubiquitin-Specific Peptidase 9 X-Linked (USP9X) Gene is Associated with Upregulation of Cyclin D1 (CCND1) and Downregulation of Cyclin-Dependent Inhibitor Kinase 1A (CDKN1A) in Breast Cancer Tissue and Cell Lines. Li H, Zheng B. Med Sci Monit. 2019 Jun 6;25:4207-4216. doi: 10.12659/MSM.914742. PubMed
Regulation of influenza A virus mRNA splicing by CLK1. Artarini A, Meyer M, Shin YJ, Huber K, Hilz N, Bracher F, Eros D, Orfi L, Keri G, Goedert S, Neuenschwander M, von Kries J, Domovich-Eisenberg Y, Dekel N, Szabadkai I, Lebendiker M, Horvath Z, Danieli T, Livnah O, Moncorge O, Frise R, Barclay W, Meyer TF, Karlas A. Antiviral Res. 2019 Aug;168:187-196. doi: 10.1016/j.antiviral.2019.06.003. Epub 2019 Jun 6. PubMed
Cas9(+) conditionally-immortalized macrophages as a tool for bacterial pathogenesis and beyond. Roberts AW, Popov LM, Mitchell G, Ching KL, Licht DJ, Golovkine G, Barton GM, Cox JS. Elife. 2019 Jun 17;8. pii: 45957. doi: 10.7554/eLife.45957. PubMed
Efficient and flexible tagging of endogenous genes by homology-independent intron targeting. Serebrenik YV, Sansbury SE, Kumar SS, Henao-Mejia J, Shalem O. Genome Res. 2019 Aug;29(8):1322-1328. doi: 10.1101/gr.246413.118. Epub 2019 Jun 25. PubMed

Associated Plasmids
FOXA1 mutations alter pioneering activity, differentiation and prostate cancer phenotypes. Adams EJ, Karthaus WR, Hoover E, Liu D, Gruet A, Zhang Z, Cho H, DiLoreto R, Chhangawala S, Liu Y, Watson PA, Davicioni E, Sboner A, Barbieri CE, Bose R, Leslie CS, Sawyers CL. Nature. 2019 Jul;571(7765):408-412. doi: 10.1038/s41586-019-1318-9. Epub 2019 Jun 26. PubMed
Rational targeting of a NuRD subcomplex guided by comprehensive in situ mutagenesis. Sher F, Hossain M, Seruggia D, Schoonenberg VAC, Yao Q, Cifani P, Dassama LMK, Cole MA, Ren C, Vinjamur DS, Macias-Trevino C, Luk K, McGuckin C, Schupp PG, Canver MC, Kurita R, Nakamura Y, Fujiwara Y, Wolfe SA, Pinello L, Maeda T, Kentsis A, Orkin SH, Bauer DE. Nat Genet. 2019 Jul;51(7):1149-1159. doi: 10.1038/s41588-019-0453-4. Epub 2019 Jun 28. PubMed
Reversible Regulation of Polyubiquitin Gene UBC via Modified Inducible CRISPR/Cas9 System. Han SW, Jung BK, Park SH, Ryu KY. Int J Mol Sci. 2019 Jun 28;20(13). pii: ijms20133168. doi: 10.3390/ijms20133168. PubMed
The lncRNA UCA1 promotes proliferation, migration, immune escape and inhibits apoptosis in gastric cancer by sponging anti-tumor miRNAs. Wang CJ, Zhu CC, Xu J, Wang M, Zhao WY, Liu Q, Zhao G, Zhang ZZ. Mol Cancer. 2019 Jul 4;18(1):115. doi: 10.1186/s12943-019-1032-0. PubMed
Combinatorial perturbation analysis reveals divergent regulations of mesenchymal genes during epithelial-to-mesenchymal transition. Watanabe K, Panchy N, Noguchi S, Suzuki H, Hong T. NPJ Syst Biol Appl. 2019 Jun 14;5:21. doi: 10.1038/s41540-019-0097-0. eCollection 2019. PubMed
Genomic and Transcriptomic Determinants of Therapy Resistance and Immune Landscape Evolution during Anti-EGFR Treatment in Colorectal Cancer. Woolston A, Khan K, Spain G, Barber LJ, Griffiths B, Gonzalez-Exposito R, Hornsteiner L, Punta M, Patil Y, Newey A, Mansukhani S, Davies MN, Furness A, Sclafani F, Peckitt C, Jimenez M, Kouvelakis K, Ranftl R, Begum R, Rana I, Thomas J, Bryant A, Quezada S, Wotherspoon A, Khan N, Fotiadis N, Marafioti T, Powles T, Lise S, Calvo F, Guettler S, von Loga K, Rao S, Watkins D, Starling N, Chau I, Sadanandam A, Cunningham D, Gerlinger M. Cancer Cell. 2019 Jul 8;36(1):35-50.e9. doi: 10.1016/j.ccell.2019.05.013. PubMed
Genome-wide CRISPR screens reveal genetic mediators of cereblon modulator toxicity in primary effusion lymphoma. Patil A, Manzano M, Gottwein E. Blood Adv. 2019 Jul 23;3(14):2105-2117. doi: 10.1182/bloodadvances.2019031732. PubMed

Associated Plasmids
Network pharmacology modeling identifies synergistic Aurora B and ZAK interaction in triple-negative breast cancer. Tang J, Gautam P, Gupta A, He L, Timonen S, Akimov Y, Wang W, Szwajda A, Jaiswal A, Turei D, Yadav B, Kankainen M, Saarela J, Saez-Rodriguez J, Wennerberg K, Aittokallio T. NPJ Syst Biol Appl. 2019 Jul 8;5:20. doi: 10.1038/s41540-019-0098-z. eCollection 2019. PubMed
Pooled library screening with multiplexed Cpf1 library. Liu J, Srinivasan S, Li CY, Ho IL, Rose J, Shaheen M, Wang G, Yao W, Deem A, Bristow C, Hart T, Draetta G. Nat Commun. 2019 Jul 17;10(1):3144. doi: 10.1038/s41467-019-10963-x. PubMed

Associated Plasmids
Construction of a mavs-inactivated MDCK cell line for facilitating the propagation of canine distemper virus (CDV). Cai C, Wang X, Zhao Y, Yi C, Jin Z, Zhang A, Han L. Mol Immunol. 2019 Oct;114:133-138. doi: 10.1016/j.molimm.2019.06.013. Epub 2019 Jul 25. PubMed
AAGAB Controls AP2 Adaptor Assembly in Clathrin-Mediated Endocytosis. Gulbranson DR, Crisman L, Lee M, Ouyang Y, Menasche BL, Demmitt BA, Wan C, Nomura T, Ye Y, Yu H, Shen J. Dev Cell. 2019 Aug 19;50(4):436-446.e5. doi: 10.1016/j.devcel.2019.06.013. Epub 2019 Jul 25. PubMed
CRISPR/Cas9 Screens Reveal Multiple Layers of B cell CD40 Regulation. Jiang C, Trudeau SJ, Cheong TC, Guo R, Teng M, Wang LW, Wang Z, Pighi C, Gautier-Courteille C, Ma Y, Jiang S, Wang C, Zhao B, Paillard L, Doench JG, Chiarle R, Gewurz BE. Cell Rep. 2019 Jul 30;28(5):1307-1322.e8. doi: 10.1016/j.celrep.2019.06.079. PubMed
A Pliable Mediator Acts as a Functional Rather Than an Architectural Bridge between Promoters and Enhancers. El Khattabi L, Zhao H, Kalchschmidt J, Young N, Jung S, Van Blerkom P, Kieffer-Kwon P, Kieffer-Kwon KR, Park S, Wang X, Krebs J, Tripathi S, Sakabe N, Sobreira DR, Huang SC, Rao SSP, Pruett N, Chauss D, Sadler E, Lopez A, Nobrega MA, Aiden EL, Asturias FJ, Casellas R. Cell. 2019 Aug 22;178(5):1145-1158.e20. doi: 10.1016/j.cell.2019.07.011. Epub 2019 Aug 8. PubMed
The Hippo pathway modulates resistance to BET proteins inhibitors in lung cancer cells. Gobbi G, Donati B, Do Valle IF, Reggiani F, Torricelli F, Remondini D, Castellani G, Ambrosetti DC, Ciarrocchi A, Sancisi V. Oncogene. 2019 Oct;38(42):6801-6817. doi: 10.1038/s41388-019-0924-1. Epub 2019 Aug 12. PubMed
Chromatin Remodeling in Response to BRCA2-Crisis. Gruber JJ, Chen J, Geller B, Jager N, Lipchik AM, Wang G, Kurian AW, Ford JM, Snyder MP. Cell Rep. 2019 Aug 20;28(8):2182-2193.e6. doi: 10.1016/j.celrep.2019.07.057. PubMed
Plasticity of the Cullin-RING Ligase Repertoire Shapes Sensitivity to Ligand-Induced Protein Degradation. Mayor-Ruiz C, Jaeger MG, Bauer S, Brand M, Sin C, Hanzl A, Mueller AC, Menche J, Winter GE. Mol Cell. 2019 Aug 22;75(4):849-858.e8. doi: 10.1016/j.molcel.2019.07.013. PubMed
Intron 1-Mediated Regulation of EGFR Expression in EGFR-Dependent Malignancies Is Mediated by AP-1 and BET Proteins. Jameson NM, Ma J, Benitez J, Izurieta A, Han JY, Mendez R, Parisian A, Furnari F. Mol Cancer Res. 2019 Nov;17(11):2208-2220. doi: 10.1158/1541-7786.MCR-19-0747. Epub 2019 Aug 23. PubMed
Control of homologous recombination by the HROB-MCM8-MCM9 pathway. Hustedt N, Saito Y, Zimmermann M, Alvarez-Quilon A, Setiaputra D, Adam S, McEwan A, Yuan JY, Olivieri M, Zhao Y, Kanemaki MT, Jurisicova A, Durocher D. Genes Dev. 2019 Oct 1;33(19-20):1397-1415. doi: 10.1101/gad.329508.119. Epub 2019 Aug 29. PubMed

Associated Plasmids
A CRISPR-based base-editing screen for the functional assessment of BRCA1 variants. Kweon J, Jang AH, Shin HR, See JE, Lee W, Lee JW, Chang S, Kim K, Kim Y. Oncogene. 2020 Jan;39(1):30-35. doi: 10.1038/s41388-019-0968-2. Epub 2019 Aug 29. PubMed
Proteomic Analysis Identifies Membrane Proteins Dependent on the ER Membrane Protein Complex. Tian S, Wu Q, Zhou B, Choi MY, Ding B, Yang W, Dong M. Cell Rep. 2019 Sep 3;28(10):2517-2526.e5. doi: 10.1016/j.celrep.2019.08.006. PubMed
A consensus set of genetic vulnerabilities to ATR inhibition. Hustedt N, Alvarez-Quilon A, McEwan A, Yuan JY, Cho T, Koob L, Hart T, Durocher D. Open Biol. 2019 Sep 27;9(9):190156. doi: 10.1098/rsob.190156. Epub 2019 Sep 11. PubMed
IFN-gamma-induced PD-L1 expression in melanoma depends on p53 expression. Thiem A, Hesbacher S, Kneitz H, di Primio T, Heppt MV, Hermanns HM, Goebeler M, Meierjohann S, Houben R, Schrama D. J Exp Clin Cancer Res. 2019 Sep 11;38(1):397. doi: 10.1186/s13046-019-1403-9. PubMed
Fascin Controls Metastatic Colonization and Mitochondrial Oxidative Phosphorylation by Remodeling Mitochondrial Actin Filaments. Lin S, Huang C, Gunda V, Sun J, Chellappan SP, Li Z, Izumi V, Fang B, Koomen J, Singh PK, Hao J, Yang S. Cell Rep. 2019 Sep 10;28(11):2824-2836.e8. doi: 10.1016/j.celrep.2019.08.011. PubMed
Iron chelation and 2-oxoglutarate-dependent dioxygenase inhibition suppress mantle cell lymphoma's cyclin D1. Babosova O, Kapralova K, Raskova Kafkova L, Korinek V, Divoky V, Prchal JT, Lanikova L. J Cell Mol Med. 2019 Nov;23(11):7785-7795. doi: 10.1111/jcmm.14655. Epub 2019 Sep 13. PubMed
Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning. Wang D, Zhang C, Wang B, Li B, Wang Q, Liu D, Wang H, Zhou Y, Shi L, Lan F, Wang Y. Nat Commun. 2019 Sep 19;10(1):4284. doi: 10.1038/s41467-019-12281-8. PubMed
HIF-independent synthetic lethality between CDK4/6 inhibition and VHL loss across species. Nicholson HE, Tariq Z, Housden BE, Jennings RB, Stransky LA, Perrimon N, Signoretti S, Kaelin WG Jr. Sci Signal. 2019 Oct 1;12(601). pii: 12/601/eaay0482. doi: 10.1126/scisignal.aay0482. PubMed

Associated Plasmids
Cell confinement reveals a branched-actin independent circuit for neutrophil polarity. Graziano BR, Town JP, Sitarska E, Nagy TL, Fosnaric M, Penic S, Iglic A, Kralj-Iglic V, Gov NS, Diz-Munoz A, Weiner OD. PLoS Biol. 2019 Oct 10;17(10):e3000457. doi: 10.1371/journal.pbio.3000457. eCollection 2019 Oct. PubMed
A one-step tRNA-CRISPR system for genome-wide genetic interaction mapping in mammalian cells. Zhao Y, Tyrishkin K, Sjaarda C, Khanal P, Stafford J, Rauh M, Liu X, Babak T, Yang X. Sci Rep. 2019 Oct 10;9(1):14499. doi: 10.1038/s41598-019-51090-3. PubMed
Mutational Analysis of Sigma-1 Receptor's Role in Synaptic Stability. Ryskamp DA, Zhemkov V, Bezprozvanny I. Front Neurosci. 2019 Sep 19;13:1012. doi: 10.3389/fnins.2019.01012. eCollection 2019. PubMed
Genome-wide CRISPR/Cas9 library screening identified PHGDH as a critical driver for Sorafenib resistance in HCC. Wei L, Lee D, Law CT, Zhang MS, Shen J, Chin DW, Zhang A, Tsang FH, Wong CL, Ng IO, Wong CC, Wong CM. Nat Commun. 2019 Oct 15;10(1):4681. doi: 10.1038/s41467-019-12606-7. PubMed
The ADP/ATP translocase drives mitophagy independent of nucleotide exchange. Hoshino A, Wang WJ, Wada S, McDermott-Roe C, Evans CS, Gosis B, Morley MP, Rathi KS, Li J, Li K, Yang S, McManus MJ, Bowman C, Potluri P, Levin M, Damrauer S, Wallace DC, Holzbaur ELF, Arany Z. Nature. 2019 Nov;575(7782):375-379. doi: 10.1038/s41586-019-1667-4. Epub 2019 Oct 16. PubMed
Nuclear receptors regulate alternative lengthening of telomeres through a novel noncanonical FANCD2 pathway. Xu M, Qin J, Wang L, Lee HJ, Kao CY, Liu D, Songyang Z, Chen J, Tsai MJ, Tsai SY. Sci Adv. 2019 Oct 9;5(10):eaax6366. doi: 10.1126/sciadv.aax6366. eCollection 2019 Oct. PubMed
Search-and-replace genome editing without double-strand breaks or donor DNA. Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR. Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 10.1038/s41586-019-1711-4. PubMed

Associated Plasmids
PIK3CA Cooperates with KRAS to Promote MYC Activity and Tumorigenesis via the Bromodomain Protein BRD9. Bell CM, Raffeiner P, Hart JR, Vogt PK. Cancers (Basel). 2019 Oct 24;11(11). pii: cancers11111634. doi: 10.3390/cancers11111634. PubMed
Antitumor immunity augments the therapeutic effects of p53 activation on acute myeloid leukemia. Hayashi Y, Goyama S, Liu X, Tamura M, Asada S, Tanaka Y, Fukuyama T, Wunderlich M, O'Brien E, Mizukawa B, Yamazaki S, Matsumoto A, Yamasaki S, Shibata T, Matsuda K, Sashida G, Takizawa H, Kitamura T. Nat Commun. 2019 Oct 25;10(1):4869. doi: 10.1038/s41467-019-12555-1. PubMed
Cholesterol accessibility at the ciliary membrane controls hedgehog signaling. Kinnebrew M, Iverson EJ, Patel BB, Pusapati GV, Kong JH, Johnson KA, Luchetti G, Eckert KM, McDonald JG, Covey DF, Siebold C, Radhakrishnan A, Rohatgi R. Elife. 2019 Oct 30;8. pii: 50051. doi: 10.7554/eLife.50051. PubMed
Biological plasticity rescues target activity in CRISPR knock outs. Smits AH, Ziebell F, Joberty G, Zinn N, Mueller WF, Clauder-Munster S, Eberhard D, Falth Savitski M, Grandi P, Jakob P, Michon AM, Sun H, Tessmer K, Burckstummer T, Bantscheff M, Steinmetz LM, Drewes G, Huber W. Nat Methods. 2019 Nov;16(11):1087-1093. doi: 10.1038/s41592-019-0614-5. Epub 2019 Oct 28. PubMed
Inducible Exoc7/Exo70 knockout reveals a critical role of the exocyst in insulin-regulated GLUT4 exocytosis. Wang S, Crisman L, Miller J, Datta I, Gulbranson DR, Tian Y, Yin Q, Yu H, Shen J. J Biol Chem. 2019 Dec 27;294(52):19988-19996. doi: 10.1074/jbc.RA119.010821. Epub 2019 Nov 18. PubMed

Associated Plasmids
GPR146 Deficiency Protects against Hypercholesterolemia and Atherosclerosis. Yu H, Rimbert A, Palmer AE, Toyohara T, Xia Y, Xia F, Ferreira LMR, Chen Z, Chen T, Loaiza N, Horwitz NB, Kacergis MC, Zhao L, Soukas AA, Kuivenhoven JA, Kathiresan S, Cowan CA. Cell. 2019 Nov 27;179(6):1276-1288.e14. doi: 10.1016/j.cell.2019.10.034. PubMed
Elucidation of WW domain ligand binding specificities in the Hippo pathway reveals STXBP4 as YAP inhibitor. Vargas RE, Duong VT, Han H, Ta AP, Chen Y, Zhao S, Yang B, Seo G, Chuc K, Oh S, El Ali A, Razorenova OV, Chen J, Luo R, Li X, Wang W. EMBO J. 2020 Jan 2;39(1):e102406. doi: 10.15252/embj.2019102406. Epub 2019 Nov 29. PubMed
Genome-wide CRISPR screen identifies ELP5 as a determinant of gemcitabine sensitivity in gallbladder cancer. Xu S, Zhan M, Jiang C, He M, Yang L, Shen H, Huang S, Huang X, Lin R, Shi Y, Liu Q, Chen W, Mohan M, Wang J. Nat Commun. 2019 Dec 2;10(1):5492. doi: 10.1038/s41467-019-13420-x. PubMed
Somatic inflammatory gene mutations in human ulcerative colitis epithelium. Nanki K, Fujii M, Shimokawa M, Matano M, Nishikori S, Date S, Takano A, Toshimitsu K, Ohta Y, Takahashi S, Sugimoto S, Ishimaru K, Kawasaki K, Nagai Y, Ishii R, Yoshida K, Sasaki N, Hibi T, Ishihara S, Kanai T, Sato T. Nature. 2020 Jan;577(7789):254-259. doi: 10.1038/s41586-019-1844-5. Epub 2019 Dec 18. PubMed
PrP is a central player in toxicity mediated by soluble aggregates of neurodegeneration-causing proteins. Corbett GT, Wang Z, Hong W, Colom-Cadena M, Rose J, Liao M, Asfaw A, Hall TC, Ding L, DeSousa A, Frosch MP, Collinge J, Harris DA, Perkinton MS, Spires-Jones TL, Young-Pearse TL, Billinton A, Walsh DM. Acta Neuropathol. 2020 Mar;139(3):503-526. doi: 10.1007/s00401-019-02114-9. Epub 2019 Dec 18. PubMed
LINC02273 drives breast cancer metastasis by epigenetically increasing AGR2 transcription. Xiu B, Chi Y, Liu L, Chi W, Zhang Q, Chen J, Guo R, Si J, Li L, Xue J, Shao ZM, Wu ZH, Huang S, Wu J. Mol Cancer. 2019 Dec 19;18(1):187. doi: 10.1186/s12943-019-1115-y. PubMed
WBP11 is required for splicing the TUBGCP6 pre-mRNA to promote centriole duplication. Park EM, Scott PM, Clutario K, Cassidy KB, Zhan K, Gerber SA, Holland AJ. J Cell Biol. 2020 Jan 6;219(1). pii: 133543. doi: 10.1083/jcb.201904203. PubMed
RNA isoform screens uncover the essentiality and tumor-suppressor activity of ultraconserved poison exons. Thomas JD, Polaski JT, Feng Q, De Neef EJ, Hoppe ER, McSharry MV, Pangallo J, Gabel AM, Belleville AE, Watson J, Nkinsi NT, Berger AH, Bradley RK. Nat Genet. 2020 Jan;52(1):84-94. doi: 10.1038/s41588-019-0555-z. Epub 2020 Jan 7. PubMed

Associated Plasmids
IQGAP1 restrains T-cell cosignaling mediated by OX40. Okuyama Y, Nagashima H, Ushio-Fukai M, Croft M, Ishii N, So T. FASEB J. 2020 Jan;34(1):540-554. doi: 10.1096/fj.201900879RR. Epub 2019 Nov 25. PubMed
Rapid non-uniform adaptation to conformation-specific KRAS(G12C) inhibition. Xue JY, Zhao Y, Aronowitz J, Mai TT, Vides A, Qeriqi B, Kim D, Li C, de Stanchina E, Mazutis L, Risso D, Lito P. Nature. 2020 Jan;577(7790):421-425. doi: 10.1038/s41586-019-1884-x. Epub 2020 Jan 8. PubMed
Genome-wide CRISPR screen identifies host dependency factors for influenza A virus infection. Li B, Clohisey SM, Chia BS, Wang B, Cui A, Eisenhaure T, Schweitzer LD, Hoover P, Parkinson NJ, Nachshon A, Smith N, Regan T, Farr D, Gutmann MU, Bukhari SI, Law A, Sangesland M, Gat-Viks I, Digard P, Vasudevan S, Lingwood D, Dockrell DH, Doench JG, Baillie JK, Hacohen N. Nat Commun. 2020 Jan 9;11(1):164. doi: 10.1038/s41467-019-13965-x. PubMed
Genome-Wide CRISPR Screen Reveals Cancer Cell Resistance to NK Cells Induced by NK-Derived IFN-gamma. Zhuang X, Veltri DP, Long EO. Front Immunol. 2019 Dec 11;10:2879. doi: 10.3389/fimmu.2019.02879. eCollection 2019. PubMed
Diverse AR Gene Rearrangements Mediate Resistance to Androgen Receptor Inhibitors in Metastatic Prostate Cancer. Li Y, Yang R, Henzler CM, Ho Y, Passow C, Auch B, Carreira S, Nava Rodrigues D, Bertan C, Hwang TH, Quigley DA, Dang HX, Morrissey C, Fraser M, Plymate SR, Maher CA, Feng FY, de Bono JS, Dehm SM. Clin Cancer Res. 2020 Apr 15;26(8):1965-1976. doi: 10.1158/1078-0432.CCR-19-3023. Epub 2020 Jan 13. PubMed
Epigenetic Silencing of CDR1as Drives IGF2BP3-Mediated Melanoma Invasion and Metastasis. Hanniford D, Ulloa-Morales A, Karz A, Berzoti-Coelho MG, Moubarak RS, Sanchez-Sendra B, Kloetgen A, Davalos V, Imig J, Wu P, Vasudevaraja V, Argibay D, Lilja K, Tabaglio T, Monteagudo C, Guccione E, Tsirigos A, Osman I, Aifantis I, Hernando E. Cancer Cell. 2020 Jan 13;37(1):55-70.e15. doi: 10.1016/j.ccell.2019.12.007. PubMed
Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages. Budzik JM, Swaney DL, Jimenez-Morales D, Johnson JR, Garelis NE, Repasy T, Roberts AW, Popov LM, Parry TJ, Pratt D, Ideker T, Krogan NJ, Cox JS. Elife. 2020 Jan 17;9. pii: 51461. doi: 10.7554/eLife.51461. PubMed
Extracellular NAD(+) enhances PARP-dependent DNA repair capacity independently of CD73 activity. Wilk A, Hayat F, Cunningham R, Li J, Garavaglia S, Zamani L, Ferraris DM, Sykora P, Andrews J, Clark J, Davis A, Chaloin L, Rizzi M, Migaud M, Sobol RW. Sci Rep. 2020 Jan 20;10(1):651. doi: 10.1038/s41598-020-57506-9. PubMed

Associated Plasmids
IL-4 controls activated neutrophil FcgammaR2b expression and migration into inflamed joints. Panda SK, Wigerblad G, Jiang L, Jimenez-Andrade Y, Iyer VS, Shen Y, Boddul SV, Guerreiro-Cacais AO, Raposo B, Kasza Z, Wermeling F. Proc Natl Acad Sci U S A. 2020 Jan 24. pii: 1914186117. doi: 10.1073/pnas.1914186117. PubMed

Associated Plasmids
Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing. Li K, Liu Y, Cao H, Zhang Y, Gu Z, Liu X, Yu A, Kaphle P, Dickerson KE, Ni M, Xu J. Nat Commun. 2020 Jan 24;11(1):485. doi: 10.1038/s41467-020-14362-5. PubMed

Associated Plasmids
Cooperation of the NEIL3 and Fanconi anemia/BRCA pathways in interstrand crosslink repair. Li N, Wang J, Wallace SS, Chen J, Zhou J, D'Andrea AD. Nucleic Acids Res. 2020 Apr 6;48(6):3014-3028. doi: 10.1093/nar/gkaa038. PubMed
The mechanism of how CD95/Fas activates the Type I IFN/STAT1 axis, driving cancer stemness in breast cancer. Qadir AS, Stults AM, Murmann AE, Peter ME. Sci Rep. 2020 Jan 28;10(1):1310. doi: 10.1038/s41598-020-58211-3. PubMed
The ARK Assay Is a Sensitive and Versatile Method for the Global Detection of DNA-Protein Crosslinks. Hu Q, Klages-Mundt N, Wang R, Lynn E, Kuma Saha L, Zhang H, Srivastava M, Shen X, Tian Y, Kim H, Ye Y, Paull T, Takeda S, Chen J, Li L. Cell Rep. 2020 Jan 28;30(4):1235-1245.e4. doi: 10.1016/j.celrep.2019.12.067. PubMed
Identification of functional regulatory elements in the human genome using pooled CRISPR screens. Borys SM, Younger ST. BMC Genomics. 2020 Jan 31;21(1):107. doi: 10.1186/s12864-020-6497-0. PubMed
Cancer Cell-Derived Matrisome Proteins Promote Metastasis in Pancreatic Ductal Adenocarcinoma. Tian C, Ohlund D, Rickelt S, Lidstrom T, Huang Y, Hao L, Zhao RT, Franklin O, Bhatia SN, Tuveson DA, Hynes RO. Cancer Res. 2020 Apr 1;80(7):1461-1474. doi: 10.1158/0008-5472.CAN-19-2578. Epub 2020 Feb 6. PubMed
A combination strategy targeting enhancer plasticity exerts synergistic lethality against BETi-resistant leukemia cells. Guo L, Li J, Zeng H, Guzman AG, Li T, Lee M, Zhou Y, Goodell MA, Stephan C, Davies PJA, Dawson MA, Sun D, Huang Y. Nat Commun. 2020 Feb 6;11(1):740. doi: 10.1038/s41467-020-14604-6. PubMed
Cell fitness screens reveal a conflict between LINE-1 retrotransposition and DNA replication. Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, Smogorzewska A, Holland AJ, Burns KH. Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 10.1038/s41594-020-0372-1. Epub 2020 Feb 10. PubMed

Associated Plasmids
Glycerol-3-phosphate is an FGF23 regulator derived from the injured kidney. Simic P, Kim W, Zhou W, Pierce KA, Chang W, Sykes DB, Aziz NB, Elmariah S, Ngo D, Pajevic PD, Govea N, Kestenbaum BR, de Boer IH, Cheng Z, Christov M, Chun J, Leaf DE, Waikar SS, Tager AM, Gerszten RE, Thadhani RI, Clish CB, Juppner H, Wein MN, Rhee EP. J Clin Invest. 2020 Mar 2;130(3):1513-1526. doi: 10.1172/JCI131190. PubMed
Local rewiring of genome-nuclear lamina interactions by transcription. Brueckner L, Zhao PA, van Schaik T, Leemans C, Sima J, Peric-Hupkes D, Gilbert DM, van Steensel B. EMBO J. 2020 Mar 16;39(6):e103159. doi: 10.15252/embj.2019103159. Epub 2020 Feb 21. PubMed
Systematic analysis of the Hippo pathway organization and oncogenic alteration in evolution. Chen Y, Han H, Seo G, Vargas RE, Yang B, Chuc K, Zhao H, Wang W. Sci Rep. 2020 Feb 21;10(1):3173. doi: 10.1038/s41598-020-60120-4. PubMed
Protein Translocation Acquires Substrate Selectivity Through ER Stress-Induced Reassembly of Translocon Auxiliary Components. Lee S, Shin Y, Kim K, Song Y, Kim Y, Kang SW. Cells. 2020 Feb 24;9(2). pii: cells9020518. doi: 10.3390/cells9020518. PubMed
A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA. de Jong OG, Murphy DE, Mager I, Willms E, Garcia-Guerra A, Gitz-Francois JJ, Lefferts J, Gupta D, Steenbeek SC, van Rheenen J, El Andaloussi S, Schiffelers RM, Wood MJA, Vader P. Nat Commun. 2020 Feb 28;11(1):1113. doi: 10.1038/s41467-020-14977-8. PubMed
Acetylation of MORC2 by NAT10 regulates cell-cycle checkpoint control and resistance to DNA-damaging chemotherapy and radiotherapy in breast cancer. Liu HY, Liu YY, Yang F, Zhang L, Zhang FL, Hu X, Shao ZM, Li DQ. Nucleic Acids Res. 2020 Apr 17;48(7):3638-3656. doi: 10.1093/nar/gkaa130. PubMed
Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5'G-extended sgRNAs. Kulcsar PI, Talas A, Toth E, Nyeste A, Ligeti Z, Welker Z, Welker E. Nat Commun. 2020 Mar 6;11(1):1223. doi: 10.1038/s41467-020-15021-5. PubMed

Associated Plasmids
CRISPR/Cas9-targeting of CD40 in hematopoietic stem cells limits immune activation mediated by anti-CD40. Wang R, Graham S, Sun N, McCarthy D, Peng R, Erickson J, Oconnor L, Zhu X, Wurbel M, Dunstan R, Westmoreland S, Chung N, Ghayur T, Gu J. PLoS One. 2020 Mar 10;15(3):e0228221. doi: 10.1371/journal.pone.0228221. eCollection 2020. PubMed
Transgenesis and Genome Editing of Mouse Spermatogonial Stem Cells by Lentivirus Pseudotyped with Sendai Virus F Protein. Shinohara T, Kanatsu-Shinohara M. Stem Cell Reports. 2020 Mar 10;14(3):447-461. doi: 10.1016/j.stemcr.2020.02.001. PubMed
Genetic Manipulation of Human Intestinal Enteroids Demonstrates the Necessity of a Functional Fucosyltransferase 2 Gene for Secretor-Dependent Human Norovirus Infection. Haga K, Ettayebi K, Tenge VR, Karandikar UC, Lewis MA, Lin SC, Neill FH, Ayyar BV, Zeng XL, Larson G, Ramani S, Atmar RL, Estes MK. mBio. 2020 Mar 17;11(2). pii: mBio.00251-20. doi: 10.1128/mBio.00251-20. PubMed
CRISPR-Cas9 Dual-gRNA Attack Causes Mutation, Excision and Inversion of the HIV-1 Proviral DNA. Binda CS, Klaver B, Berkhout B, Das AT. Viruses. 2020 Mar 18;12(3). pii: v12030330. doi: 10.3390/v12030330. PubMed
CD20 is dispensable for B-cell receptor signaling but is required for proper actin polymerization, adhesion and migration of malignant B cells. Kozlova V, Ledererova A, Ladungova A, Peschelova H, Janovska P, Slusarczyk A, Domagala J, Kopcil P, Vakulova V, Oppelt J, Bryja V, Doubek M, Mayer J, Pospisilova S, Smida M. PLoS One. 2020 Mar 25;15(3):e0229170. doi: 10.1371/journal.pone.0229170. eCollection 2020. PubMed
Heparan sulfate is a clearance receptor for aberrant extracellular proteins. Itakura E, Chiba M, Murata T, Matsuura A. J Cell Biol. 2020 Mar 2;219(3). pii: 133807. doi: 10.1083/jcb.201911126. PubMed
Targeting clinical epigenetic reprogramming for chemoprevention of metabolic and viral hepatocellular carcinoma. Juhling F, Hamdane N, Crouchet E, Li S, El Saghire H, Mukherji A, Fujiwara N, Oudot MA, Thumann C, Saviano A, Roca Suarez AA, Goto K, Masia R, Sojoodi M, Arora G, Aikata H, Ono A, Tabrizian P, Schwartz M, Polyak SJ, Davidson I, Schmidl C, Bock C, Schuster C, Chayama K, Pessaux P, Tanabe KK, Hoshida Y, Zeisel MB, Duong FH, Fuchs BC, Baumert TF. Gut. 2021 Jan;70(1):157-169. doi: 10.1136/gutjnl-2019-318918. Epub 2020 Mar 26. PubMed
Performance of anti-CD19 chimeric antigen receptor T cells in genetically defined classes of chronic lymphocytic leukemia. Mancikova V, Peschelova H, Kozlova V, Ledererova A, Ladungova A, Verner J, Loja T, Folber F, Mayer J, Pospisilova S, Smida M. J Immunother Cancer. 2020 Mar;8(1). pii: jitc-2019-000471. doi: 10.1136/jitc-2019-000471. PubMed
CD21 (Complement Receptor 2) Is the Receptor for Epstein-Barr Virus Entry into T Cells. Smith NA, Coleman CB, Gewurz BE, Rochford R. J Virol. 2020 May 18;94(11). pii: JVI.00428-20. doi: 10.1128/JVI.00428-20. Print 2020 May 18. PubMed
Loss of N-Glycanase 1 Alters Transcriptional and Translational Regulation in K562 Cell Lines. Mueller WF, Jakob P, Sun H, Clauder-Munster S, Ghidelli-Disse S, Ordonez D, Boesche M, Bantscheff M, Collier P, Haase B, Benes V, Paulsen M, Sehr P, Lewis J, Drewes G, Steinmetz LM. G3 (Bethesda). 2020 May 4;10(5):1585-1597. doi: 10.1534/g3.119.401031. PubMed
Genome-Wide CRISPR Screen Identifies Semaphorin 6A and 6B as Receptors for Paeniclostridium sordellii Toxin TcsL. Tian S, Liu Y, Wu H, Liu H, Zeng J, Choi MY, Chen H, Gerhard R, Dong M. Cell Host Microbe. 2020 May 13;27(5):782-792.e7. doi: 10.1016/j.chom.2020.03.007. Epub 2020 Apr 16. PubMed
Endocrine-Exocrine Signaling Drives Obesity-Associated Pancreatic Ductal Adenocarcinoma. Chung KM, Singh J, Lawres L, Dorans KJ, Garcia C, Burkhardt DB, Robbins R, Bhutkar A, Cardone R, Zhao X, Babic A, Vayrynen SA, Dias Costa A, Nowak JA, Chang DT, Dunne RF, Hezel AF, Koong AC, Wilhelm JJ, Bellin MD, Nylander V, Gloyn AL, McCarthy MI, Kibbey RG, Krishnaswamy S, Wolpin BM, Jacks T, Fuchs CS, Muzumdar MD. Cell. 2020 May 14;181(4):832-847.e18. doi: 10.1016/j.cell.2020.03.062. Epub 2020 Apr 17. PubMed
Distinct roles of BRCA2 in replication fork protection in response to hydroxyurea and DNA interstrand cross-links. Rickman KA, Noonan RJ, Lach FP, Sridhar S, Wang AT, Abhyankar A, Huang A, Kelly M, Auerbach AD, Smogorzewska A. Genes Dev. 2020 Jun 1;34(11-12):832-846. doi: 10.1101/gad.336446.120. Epub 2020 Apr 30. PubMed
Hdac3 is an epigenetic inhibitor of the cytotoxicity program in CD8 T cells. Tay RE, Olawoyin O, Cejas P, Xie Y, Meyer CA, Weng QY, Fisher DE, Long HW, Brown M, Kim HJ, Wucherpfennig KW. J Exp Med. 2020 Jul 6;217(7). pii: 151741. doi: 10.1084/jem.20191453. PubMed
Biochemical characteristics of the chondrocyte-enriched SNORC protein and its transcriptional regulation by SOX9. Jaiswal PK, Aljebali L, Gaumond MH, Oh CD, Yasuda H, Moffatt P. Sci Rep. 2020 May 8;10(1):7790. doi: 10.1038/s41598-020-64640-x. PubMed
LINC00261 Is Differentially Expressed in Pancreatic Cancer Subtypes and Regulates a Pro-Epithelial Cell Identity. Dorn A, Glass M, Neu CT, Heydel B, Huttelmaier S, Gutschner T, Haemmerle M. Cancers (Basel). 2020 May 13;12(5). pii: cancers12051227. doi: 10.3390/cancers12051227. PubMed
Direct Antiviral Activity of IFN-Stimulated Genes Is Responsible for Resistance to Paramyxoviruses in ISG15-Deficient Cells. Holthaus D, Vasou A, Bamford CGG, Andrejeva J, Paulus C, Randall RE, McLauchlan J, Hughes DJ. J Immunol. 2020 Jul 1;205(1):261-271. doi: 10.4049/jimmunol.1901472. Epub 2020 May 18. PubMed
Sense-antisense miRNA pairs constitute an elaborate reciprocal regulatory circuit. Song Y, Li L, Yang W, Fu Q, Chen W, Fang Z, Li W, Gu N, Zhang R. Genome Res. 2020 May;30(5):661-672. doi: 10.1101/gr.257121.119. Epub 2020 May 18. PubMed
DNA methylation enzymes and PRC1 restrict B-cell Epstein-Barr virus oncoprotein expression. Guo R, Zhang Y, Teng M, Jiang C, Schineller M, Zhao B, Doench JG, O'Reilly RJ, Cesarman E, Giulino-Roth L, Gewurz BE. Nat Microbiol. 2020 Aug;5(8):1051-1063. doi: 10.1038/s41564-020-0724-y. Epub 2020 May 18. PubMed
Loss of heterozygosity of essential genes represents a widespread class of potential cancer vulnerabilities. Nichols CA, Gibson WJ, Brown MS, Kosmicki JA, Busanovich JP, Wei H, Urbanski LM, Curimjee N, Berger AC, Gao GF, Cherniack AD, Dhe-Paganon S, Paolella BR, Beroukhim R. Nat Commun. 2020 May 20;11(1):2517. doi: 10.1038/s41467-020-16399-y. PubMed
Mitochondrial phosphatase PGAM5 modulates cellular senescence by regulating mitochondrial dynamics. Yu B, Ma J, Li J, Wang D, Wang Z, Wang S. Nat Commun. 2020 May 21;11(1):2549. doi: 10.1038/s41467-020-16312-7. PubMed
Targeting E. coli invasion of the blood-brain barrier for investigating the pathogenesis and therapeutic development of E. coli meningitis. Zhu N, Liu W, Prakash A, Zhang C, Kim KS. Cell Microbiol. 2020 May 24:e13231. doi: 10.1111/cmi.13231. PubMed
HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription. Zhu X, Lan B, Yi X, He C, Dang L, Zhou X, Lu Y, Sun Y, Liu Z, Bai X, Zhang K, Li B, Li MJ, Chen Y, Zhang L. Nucleic Acids Res. 2020 Jul 9;48(12):6563-6582. doi: 10.1093/nar/gkaa441. PubMed
CRISPR screen in regulatory T cells reveals modulators of Foxp3. Cortez JT, Montauti E, Shifrut E, Gatchalian J, Zhang Y, Shaked O, Xu Y, Roth TL, Simeonov DR, Zhang Y, Chen S, Li Z, Woo JM, Ho J, Vogel IA, Prator GY, Zhang B, Lee Y, Sun Z, Ifergan I, Van Gool F, Hargreaves DC, Bluestone JA, Marson A, Fang D. Nature. 2020 Jun;582(7812):416-420. doi: 10.1038/s41586-020-2246-4. Epub 2020 Apr 29. PubMed
LRRK2 Kinase Inhibition Rescues Deficits in Lysosome Function Due to Heterozygous GBA1 Expression in Human iPSC-Derived Neurons. Sanyal A, Novis HS, Gasser E, Lin S, LaVoie MJ. Front Neurosci. 2020 May 15;14:442. doi: 10.3389/fnins.2020.00442. eCollection 2020. PubMed
Tumor Interferon Signaling Is Regulated by a lncRNA INCR1 Transcribed from the PD-L1 Locus. Mineo M, Lyons SM, Zdioruk M, von Spreckelsen N, Ferrer-Luna R, Ito H, Alayo QA, Kharel P, Giantini Larsen A, Fan WY, Auduong S, Grauwet K, Passaro C, Khalsa JK, Shah K, Reardon DA, Ligon KL, Beroukhim R, Nakashima H, Ivanov P, Anderson PJ, Lawler SE, Chiocca EA. Mol Cell. 2020 Jun 18;78(6):1207-1223.e8. doi: 10.1016/j.molcel.2020.05.015. Epub 2020 Jun 5. PubMed
Direct-seq: programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen. Song Q, Ni K, Liu M, Li Y, Wang L, Wang Y, Liu Y, Yu Z, Qi Y, Lu Z, Ma L. Genome Biol. 2020 Jun 8;21(1):136. doi: 10.1186/s13059-020-02044-w. PubMed

Associated Plasmids
Massively parallel Cas13 screens reveal principles for guide RNA design. Wessels HH, Mendez-Mancilla A, Guo X, Legut M, Daniloski Z, Sanjana NE. Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16. PubMed

Associated Plasmids
A Genome-Wide CRISPR-Cas9 Screen Reveals the Requirement of Host Cell Sulfation for Schmallenberg Virus Infection. Thamamongood T, Aebischer A, Wagner V, Chang MW, Elling R, Benner C, Garcia-Sastre A, Kochs G, Beer M, Schwemmle M. J Virol. 2020 Aug 17;94(17). pii: JVI.00752-20. doi: 10.1128/JVI.00752-20. Print 2020 Aug 17. PubMed
The Tumour Suppressor TMEM127 Is a Nedd4-Family E3 Ligase Adaptor Required by Salmonella SteD to Ubiquitinate and Degrade MHC Class II Molecules. Alix E, Godlee C, Cerny O, Blundell S, Tocci R, Matthews S, Liu M, Pruneda JN, Swatek KN, Komander D, Sleap T, Holden DW. Cell Host Microbe. 2020 Jul 8;28(1):54-68.e7. doi: 10.1016/j.chom.2020.04.024. Epub 2020 Jun 10. PubMed
Targeting FTO Suppresses Cancer Stem Cell Maintenance and Immune Evasion. Su R, Dong L, Li Y, Gao M, Han L, Wunderlich M, Deng X, Li H, Huang Y, Gao L, Li C, Zhao Z, Robinson S, Tan B, Qing Y, Qin X, Prince E, Xie J, Qin H, Li W, Shen C, Sun J, Kulkarni P, Weng H, Huang H, Chen Z, Zhang B, Wu X, Olsen MJ, Muschen M, Marcucci G, Salgia R, Li L, Fathi AT, Li Z, Mulloy JC, Wei M, Horne D, Chen J. Cancer Cell. 2020 Jul 13;38(1):79-96.e11. doi: 10.1016/j.ccell.2020.04.017. Epub 2020 Jun 11. PubMed
Repurposing type I-F CRISPR-Cas system as a transcriptional activation tool in human cells. Chen Y, Liu J, Zhi S, Zheng Q, Ma W, Huang J, Liu Y, Liu D, Liang P, Songyang Z. Nat Commun. 2020 Jun 19;11(1):3136. doi: 10.1038/s41467-020-16880-8. PubMed

Associated Plasmids
MAP4K Interactome Reveals STRN4 as a Key STRIPAK Complex Component in Hippo Pathway Regulation. Seo G, Han H, Vargas RE, Yang B, Li X, Wang W. Cell Rep. 2020 Jul 7;32(1):107860. doi: 10.1016/j.celrep.2020.107860. PubMed
A Genetic Map of the Response to DNA Damage in Human Cells. Olivieri M, Cho T, Alvarez-Quilon A, Li K, Schellenberg MJ, Zimmermann M, Hustedt N, Rossi SE, Adam S, Melo H, Heijink AM, Sastre-Moreno G, Moatti N, Szilard RK, McEwan A, Ling AK, Serrano-Benitez A, Ubhi T, Feng S, Pawling J, Delgado-Sainz I, Ferguson MW, Dennis JW, Brown GW, Cortes-Ledesma F, Williams RS, Martin A, Xu D, Durocher D. Cell. 2020 Jul 23;182(2):481-496.e21. doi: 10.1016/j.cell.2020.05.040. Epub 2020 Jul 9. PubMed
SARS-CoV-2 Infection Depends on Cellular Heparan Sulfate and ACE2. Clausen TM, Sandoval DR, Spliid CB, Pihl J, Painter CD, Thacker BE, Glass CA, Narayanan A, Majowicz SA, Zhang Y, Torres JL, Golden GJ, Porell R, Garretson AF, Laubach L, Feldman J, Yin X, Pu Y, Hauser B, Caradonna TM, Kellman BP, Martino C, Gordts PLSM, Leibel SL, Chanda SK, Schmidt AG, Godula K, Jose J, Corbett KD, Ward AB, Carlin AF, Esko JD. bioRxiv. 2020 Jul 14. doi: 10.1101/2020.07.14.201616. PubMed
TRAIL-conjugated silver nanoparticles sensitize glioblastoma cells to TRAIL by regulating CHK1 in the DNA repair pathway. Sur-Erdem I, Muslu K, Pinarbasi N, Altunbek M, Seker-Polat F, Cingoz A, Aydin SO, Kahraman M, Culha M, Solaroglu I, Bagci-Onder T. Neurol Res. 2020 Dec;42(12):1061-1069. doi: 10.1080/01616412.2020.1796378. Epub 2020 Jul 25. PubMed
DNA methylation repels binding of hypoxia-inducible transcription factors to maintain tumor immunotolerance. D'Anna F, Van Dyck L, Xiong J, Zhao H, Berrens RV, Qian J, Bieniasz-Krzywiec P, Chandra V, Schoonjans L, Matthews J, De Smedt J, Minnoye L, Amorim R, Khorasanizadeh S, Yu Q, Zhao L, De Borre M, Savvides SN, Simon MC, Carmeliet P, Reik W, Rastinejad F, Mazzone M, Thienpont B, Lambrechts D. Genome Biol. 2020 Jul 27;21(1):182. doi: 10.1186/s13059-020-02087-z. PubMed
ABHD11 Is Critical for Embryonic Stem Cell Expansion, Differentiation and Lipid Metabolic Homeostasis. Liu G, Ruan Y, Zhang J, Wang X, Wu W, He P, Wang J, Xiong J, Cheng Y, Liu L, Yang Y, Tian Y, Jian R. Front Cell Dev Biol. 2020 Jul 7;8:570. doi: 10.3389/fcell.2020.00570. eCollection 2020. PubMed
Genetic evidence for an inhibitory role of tomosyn in insulin-stimulated GLUT4 exocytosis. Wang S, Liu Y, Crisman L, Wan C, Miller J, Yu H, Shen J. Traffic. 2020 Oct;21(10):636-646. doi: 10.1111/tra.12760. PubMed
Cell Cycle Checkpoints Cooperate to Suppress DNA- and RNA-Associated Molecular Pattern Recognition and Anti-Tumor Immune Responses. Chen J, Harding SM, Natesan R, Tian L, Benci JL, Li W, Minn AJ, Asangani IA, Greenberg RA. Cell Rep. 2020 Sep 1;32(9):108080. doi: 10.1016/j.celrep.2020.108080. PubMed
A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis. Zhang Q, Cao C, Gong W, Bao K, Wang Q, Wang Y, Bi L, Ma S, Zhao J, Liu L, Tian S, Zhang K, Yang J, Yao Z, Song N, Shi L. Theranostics. 2020 Aug 29;10(23):10769-10790. doi: 10.7150/thno.46878. eCollection 2020. PubMed
SARS-CoV-2 Infection Depends on Cellular Heparan Sulfate and ACE2. Clausen TM, Sandoval DR, Spliid CB, Pihl J, Perrett HR, Painter CD, Narayanan A, Majowicz SA, Kwong EM, McVicar RN, Thacker BE, Glass CA, Yang Z, Torres JL, Golden GJ, Bartels PL, Porell RN, Garretson AF, Laubach L, Feldman J, Yin X, Pu Y, Hauser BM, Caradonna TM, Kellman BP, Martino C, Gordts PLSM, Chanda SK, Schmidt AG, Godula K, Leibel SL, Jose J, Corbett KD, Ward AB, Carlin AF, Esko JD. Cell. 2020 Nov 12;183(4):1043-1057.e15. doi: 10.1016/j.cell.2020.09.033. Epub 2020 Sep 14. PubMed
A web tool for the design of prime-editing guide RNAs. Chow RD, Chen JS, Shen J, Chen S. Nat Biomed Eng. 2021 Feb;5(2):190-194. doi: 10.1038/s41551-020-00622-8. Epub 2020 Sep 28. PubMed
A saturating mutagenesis CRISPR-Cas9-mediated functional genomic screen identifies cis- and trans-regulatory elements of Oct4 in murine ESCs. Canver MC, Tripathi P, Bullen MJ, Olshansky M, Kumar Y, Wong LH, Turner SJ, Lessard S, Pinello L, Orkin SH, Das PP. J Biol Chem. 2020 Nov 20;295(47):15797-15809. doi: 10.1074/jbc.RA120.013772. Epub 2020 Sep 29. PubMed
Functional interrogation of HOXA9 regulome in MLLr leukemia via reporter-based CRISPR/Cas9 screen. Zhang H, Zhang Y, Zhou X, Wright S, Hyle J, Zhao L, An J, Zhao X, Shao Y, Xu B, Lee HM, Chen T, Zhou Y, Chen X, Lu R, Li C. Elife. 2020 Oct 1;9. pii: 57858. doi: 10.7554/eLife.57858. PubMed

Associated Plasmids
BRD4 modulates vulnerability of triple-negative breast cancer to targeting of integrin-dependent signaling pathways. Zhang Y, Xu B, Shi J, Li J, Lu X, Xu L, Yang H, Hamad N, Wang C, Napier D, He S, Liu C, Liu Z, Qian H, Chen L, Wei X, Zheng X, Huang JA, Thibault O, Craven R, Wei D, Pan Y, Zhou BP, Wu Y, Yang XH. Cell Oncol (Dordr). 2020 Dec;43(6):1049-1066. doi: 10.1007/s13402-020-00537-1. Epub 2020 Oct 2. PubMed
FBXO11 is a candidate tumor suppressor in the leukemic transformation of myelodysplastic syndrome. Schieber M, Marinaccio C, Bolanos LC, Haffey WD, Greis KD, Starczynowski DT, Crispino JD. Blood Cancer J. 2020 Oct 6;10(10):98. doi: 10.1038/s41408-020-00362-7. PubMed
Truncated HDAC9 identified by integrated genome-wide screen as the key modulator for paclitaxel resistance in triple-negative breast cancer. Lian B, Pei YC, Jiang YZ, Xue MZ, Li DQ, Li XG, Zheng YZ, Liu XY, Qiao F, Sun WL, Ling H, He M, Yao L, Hu X, Shao ZM. Theranostics. 2020 Sep 2;10(24):11092-11109. doi: 10.7150/thno.44997. eCollection 2020. PubMed
Endogenous retroviruses drive KRAB zinc-finger protein family expression for tumor suppression. Ito J, Kimura I, Soper A, Coudray A, Koyanagi Y, Nakaoka H, Inoue I, Turelli P, Trono D, Sato K. Sci Adv. 2020 Oct 21;6(43). pii: 6/43/eabc3020. doi: 10.1126/sciadv.abc3020. Print 2020 Oct. PubMed
Type I interferon signaling mediates Mycobacterium tuberculosis-induced macrophage death. Zhang L, Jiang X, Pfau D, Ling Y, Nathan CF. J Exp Med. 2021 Feb 1;218(2). pii: 211515. doi: 10.1084/jem.20200887. PubMed
Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection. Wei J, Alfajaro MM, DeWeirdt PC, Hanna RE, Lu-Culligan WJ, Cai WL, Strine MS, Zhang SM, Graziano VR, Schmitz CO, Chen JS, Mankowski MC, Filler RB, Ravindra NG, Gasque V, de Miguel FJ, Patil A, Chen H, Oguntuyo KY, Abriola L, Surovtseva YV, Orchard RC, Lee B, Lindenbach BD, Politi K, van Dijk D, Kadoch C, Simon MD, Yan Q, Doench JG, Wilen CB. Cell. 2021 Jan 7;184(1):76-91.e13. doi: 10.1016/j.cell.2020.10.028. Epub 2020 Oct 20. PubMed

Associated Plasmids
G9a Promotes Breast Cancer Recurrence through Repression of a Pro-inflammatory Program. Mabe NW, Garcia NMG, Wolery SE, Newcomb R, Meingasner RC, Vilona BA, Lupo R, Lin CC, Chi JT, Alvarez JV. Cell Rep. 2020 Nov 3;33(5):108341. doi: 10.1016/j.celrep.2020.108341. PubMed
The hexosamine biosynthesis pathway is a targetable liability in KRAS/LKB1 mutant lung cancer. Kim J, Lee HM, Cai F, Ko B, Yang C, Lieu EL, Muhammad N, Rhyne S, Li K, Haloul M, Gu W, Faubert B, Kaushik AK, Cai L, Kasiri S, Marriam U, Nham K, Girard L, Wang H, Sun X, Kim J, Minna JD, Unsal-Kacmaz K, DeBerardinis RJ. Nat Metab. 2020 Dec;2(12):1401-1412. doi: 10.1038/s42255-020-00316-0. Epub 2020 Nov 30. PubMed
Functional characterization of uveal melanoma oncogenes. Ma J, Weng L, Bastian BC, Chen X. Oncogene. 2021 Jan;40(4):806-820. doi: 10.1038/s41388-020-01569-5. Epub 2020 Dec 1. PubMed
CRISPR screen identifies genes that sensitize AML cells to double-negative T-cell therapy. Soares F, Chen B, Lee JB, Ahmed M, Ly D, Tin E, Kang H, Zeng Y, Akhtar N, Minden MD, He HH, Zhang L. Blood. 2021 Apr 22;137(16):2171-2181. doi: 10.1182/blood.2019004108. PubMed
Muscle progenitor specification and myogenic differentiation are associated with changes in chromatin topology. Zhang N, Mendieta-Esteban J, Magli A, Lilja KC, Perlingeiro RCR, Marti-Renom MA, Tsirigos A, Dynlacht BD. Nat Commun. 2020 Dec 4;11(1):6222. doi: 10.1038/s41467-020-19999-w. PubMed
The long non-coding RNA LUCAT1 is a negative feedback regulator of interferon responses in humans. Agarwal S, Vierbuchen T, Ghosh S, Chan J, Jiang Z, Kandasamy RK, Ricci E, Fitzgerald KA. Nat Commun. 2020 Dec 11;11(1):6348. doi: 10.1038/s41467-020-20165-5. PubMed
TMEM41B Is a Pan-flavivirus Host Factor. Hoffmann HH, Schneider WM, Rozen-Gagnon K, Miles LA, Schuster F, Razooky B, Jacobson E, Wu X, Yi S, Rudin CM, MacDonald MR, McMullan LK, Poirier JT, Rice CM. Cell. 2021 Jan 7;184(1):133-148.e20. doi: 10.1016/j.cell.2020.12.005. Epub 2020 Dec 9. PubMed
A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression. Huh D, Passarelli MC, Gao J, Dusmatova SN, Goin C, Fish L, Pinzaru AM, Molina H, Ren Z, McMillan EA, Asgharian H, Goodarzi H, Tavazoie SF. EMBO J. 2021 Jan 15;40(2):e106696. doi: 10.15252/embj.2020106696. Epub 2020 Dec 21. PubMed
Manipulation of the human tRNA pool reveals distinct tRNA sets that act in cellular proliferation or cell cycle arrest. Aharon-Hefetz N, Frumkin I, Mayshar Y, Dahan O, Pilpel Y, Rak R. Elife. 2020 Dec 24;9. pii: 58461. doi: 10.7554/eLife.58461. PubMed
TMEM70 forms oligomeric scaffolds within mitochondrial cristae promoting in situ assembly of mammalian ATP synthase proton channel. Bahri H, Buratto J, Rojo M, Dompierre JP, Salin B, Blancard C, Cuvellier S, Rose M, Ben Ammar Elgaaied A, Tetaud E, di Rago JP, Devin A, Duvezin-Caubet S. Biochim Biophys Acta Mol Cell Res. 2021 Apr;1868(4):118942. doi: 10.1016/j.bbamcr.2020.118942. Epub 2020 Dec 23. PubMed
UFMylation inhibits the proinflammatory capacity of interferon-gamma-activated macrophages. Balce DR, Wang YT, McAllaster MR, Dunlap BF, Orvedahl A, Hykes BL Jr, Droit L, Handley SA, Wilen CB, Doench JG, Orchard RC, Stallings CL, Virgin HW. Proc Natl Acad Sci U S A. 2021 Jan 5;118(1). pii: 2011763118. doi: 10.1073/pnas.2011763118. PubMed
Genome-Scale Identification of SARS-CoV-2 and Pan-coronavirus Host Factor Networks. Schneider WM, Luna JM, Hoffmann HH, Sanchez-Rivera FJ, Leal AA, Ashbrook AW, Le Pen J, Ricardo-Lax I, Michailidis E, Peace A, Stenzel AF, Lowe SW, MacDonald MR, Rice CM, Poirier JT. Cell. 2021 Jan 7;184(1):120-132.e14. doi: 10.1016/j.cell.2020.12.006. Epub 2020 Dec 9. PubMed
Xpo7 negatively regulates Hedgehog signaling by exporting Gli2 from the nucleus. Markiewicz L, Uspienski T, Baran B, Niedziolka SM, Niewiadomski P. Cell Signal. 2021 Apr;80:109907. doi: 10.1016/j.cellsig.2020.109907. Epub 2020 Dec 28. PubMed
Functional Genomics Identify Distinct and Overlapping Genes Mediating Resistance to Different Classes of Heterobifunctional Degraders of Oncoproteins. Shirasaki R, Matthews GM, Gandolfi S, de Matos Simoes R, Buckley DL, Raja Vora J, Sievers QL, Bruggenthies JB, Dashevsky O, Poarch H, Tang H, Bariteau MA, Sheffer M, Hu Y, Downey-Kopyscinski SL, Hengeveld PJ, Glassner BJ, Dhimolea E, Ott CJ, Zhang T, Kwiatkowski NP, Laubach JP, Schlossman RL, Richardson PG, Culhane AC, Groen RWJ, Fischer ES, Vazquez F, Tsherniak A, Hahn WC, Levy J, Auclair D, Licht JD, Keats JJ, Boise LH, Ebert BL, Bradner JE, Gray NS, Mitsiades CS. Cell Rep. 2021 Jan 5;34(1):108532. doi: 10.1016/j.celrep.2020.108532. PubMed
Direct Intracellular Visualization of Ebola Virus-Receptor Interaction by In Situ Proximity Ligation. Mittler E, Alkutkar T, Jangra RK, Chandran K. mBio. 2021 Jan 12;12(1). pii: mBio.03100-20. doi: 10.1128/mBio.03100-20. PubMed
m(6)A RNA methylation regulates the fate of endogenous retroviruses. Chelmicki T, Roger E, Teissandier A, Dura M, Bonneville L, Rucli S, Dossin F, Fouassier C, Lameiras S, Bourc'his D. Nature. 2021 Mar;591(7849):312-316. doi: 10.1038/s41586-020-03135-1. Epub 2021 Jan 13. PubMed
Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying functional circRNAs. Zhang Y, Nguyen TM, Zhang XO, Wang L, Phan T, Clohessy JG, Pandolfi PP. Genome Biol. 2021 Jan 21;22(1):41. doi: 10.1186/s13059-021-02263-9. PubMed
G3BPs tether the TSC complex to lysosomes and suppress mTORC1 signaling. Prentzell MT, Rehbein U, Cadena Sandoval M, De Meulemeester AS, Baumeister R, Brohee L, Berdel B, Bockwoldt M, Carroll B, Chowdhury SR, von Deimling A, Demetriades C, Figlia G, de Araujo MEG, Heberle AM, Heiland I, Holzwarth B, Huber LA, Jaworski J, Kedra M, Kern K, Kopach A, Korolchuk VI, van 't Land-Kuper I, Macias M, Nellist M, Palm W, Pusch S, Ramos Pittol JM, Reil M, Reintjes A, Reuter F, Sampson JR, Scheldeman C, Siekierska A, Stefan E, Teleman AA, Thomas LE, Torres-Quesada O, Trump S, West HD, de Witte P, Woltering S, Yordanov TE, Zmorzynska J, Opitz CA, Thedieck K. Cell. 2021 Feb 4;184(3):655-674.e27. doi: 10.1016/j.cell.2020.12.024. Epub 2021 Jan 25. PubMed
CRISPRi enables isoform-specific loss-of-function screens and identification of gastric cancer-specific isoform dependencies. Davies R, Liu L, Taotao S, Tuano N, Chaturvedi R, Huang KK, Itman C, Mandoli A, Qamra A, Hu C, Powell D, Daly RJ, Tan P, Rosenbluh J. Genome Biol. 2021 Jan 26;22(1):47. doi: 10.1186/s13059-021-02266-6. PubMed
SARS-CoV-2 triggers an MDA-5-dependent interferon response which is unable to control replication in lung epithelial cells. Rebendenne A, Valadao ALC, Tauziet M, Maarifi G, Bonaventure B, McKellar J, Planes R, Nisole S, Arnaud-Arnould M, Moncorge O, Goujon C. J Virol. 2021 Jan 29. pii: JVI.02415-20. doi: 10.1128/JVI.02415-20. PubMed

Associated Plasmids
LETR1 is a lymphatic endothelial-specific lncRNA governing cell proliferation and migration through KLF4 and SEMA3C. Ducoli L, Agrawal S, Sibler E, Kouno T, Tacconi C, Hon CC, Berger SD, Mullhaupt D, He Y, Kim J, D'Addio M, Dieterich LC, Carninci P, de Hoon MJL, Shin JW, Detmar M. Nat Commun. 2021 Feb 10;12(1):925. doi: 10.1038/s41467-021-21217-0. PubMed
Advanced single-cell pooled CRISPR screening identifies C19orf53 required for cell proliferation based on mTORC1 regulators. Tang Y, Liao S, Liu G, Xiong X, Liu H, Li F, Tan Z, Kong X, Yin Y, Tan B. Cell Biol Toxicol. 2021 Feb 13. pii: 10.1007/s10565-021-09586-0. doi: 10.1007/s10565-021-09586-0. PubMed
Modulating HSF1 levels impacts expression of the estrogen receptor alpha and antiestrogen response. Silveira MA, Tav C, Berube-Simard FA, Cuppens T, Leclercq M, Fournier E, Cote MC, Droit A, Bilodeau S. Life Sci Alliance. 2021 Feb 16;4(5). pii: 4/5/e202000811. doi: 10.26508/lsa.202000811. Print 2021 May. PubMed
Functional interrogation of DNA damage response variants with base editing screens. Cuella-Martin R, Hayward SB, Fan X, Chen X, Huang JW, Taglialatela A, Leuzzi G, Zhao J, Rabadan R, Lu C, Shen Y, Ciccia A. Cell. 2021 Feb 18;184(4):1081-1097.e19. doi: 10.1016/j.cell.2021.01.041. PubMed
Susceptibility-Associated Genetic Variation in NEDD9 Contributes to Prostate Cancer Initiation and Progression. Han D, Owiredu JN, Healy BM, Li M, Labaf M, Steinfeld JS, Patalano S, Gao S, Liu M, Macoska JA, Zarringhalam K, Siegfried KR, Yuan X, Rebbeck TR, Cai C. Cancer Res. 2021 Jul 15;81(14):3766-3776. doi: 10.1158/0008-5472.CAN-20-3042. Epub 2021 Feb 25. PubMed
In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8(+) T cell fate decisions. Huang H, Zhou P, Wei J, Long L, Shi H, Dhungana Y, Chapman NM, Fu G, Saravia J, Raynor JL, Liu S, Palacios G, Wang YD, Qian C, Yu J, Chi H. Cell. 2021 Mar 4;184(5):1245-1261.e21. doi: 10.1016/j.cell.2021.02.021. Epub 2021 Feb 25. PubMed
PlasmidHawk improves lab of origin prediction of engineered plasmids using sequence alignment. Wang Q, Kille B, Liu TR, Elworth RAL, Treangen TJ. Nat Commun. 2021 Feb 26;12(1):1167. doi: 10.1038/s41467-021-21180-w. PubMed
Transcription factor competition at the gamma-globin promoters controls hemoglobin switching. Liu N, Xu S, Yao Q, Zhu Q, Kai Y, Hsu JY, Sakon P, Pinello L, Yuan GC, Bauer DE, Orkin SH. Nat Genet. 2021 Apr;53(4):511-520. doi: 10.1038/s41588-021-00798-y. Epub 2021 Mar 1. PubMed
Engineered IgG1-Fc Molecules Define Valency Control of Cell Surface Fcgamma Receptor Inhibition and Activation in Endosomes. Bailey EM, Choudhury A, Vuppula H, Ortiz DF, Schaeck J, Manning AM, Bosques CJ, Hoppe AD. Front Immunol. 2021 Feb 15;11:617767. doi: 10.3389/fimmu.2020.617767. eCollection 2020. PubMed
Identification of pathogenic variants in cancer genes using base editing screens with editing efficiency correction. Huang C, Li G, Wu J, Liang J, Wang X. Genome Biol. 2021 Mar 10;22(1):80. doi: 10.1186/s13059-021-02305-2. PubMed
SARS-CoV-2 hijacks folate and one-carbon metabolism for viral replication. Zhang Y, Guo R, Kim SH, Shah H, Zhang S, Liang JH, Fang Y, Gentili M, Leary CNO, Elledge SJ, Hung DT, Mootha VK, Gewurz BE. Nat Commun. 2021 Mar 15;12(1):1676. doi: 10.1038/s41467-021-21903-z. PubMed
Caspase cleavage releases a nuclear protein fragment that stimulates phospholipid scrambling at the plasma membrane. Maruoka M, Zhang P, Mori H, Imanishi E, Packwood DM, Harada H, Kosako H, Suzuki J. Mol Cell. 2021 Apr 1;81(7):1397-1410.e9. doi: 10.1016/j.molcel.2021.02.025. Epub 2021 Mar 15. PubMed
A hierarchical regulatory network analysis of the vitamin D induced transcriptome reveals novel regulators and complete VDR dependency in monocytes. Warwick T, Schulz MH, Gunther S, Gilsbach R, Neme A, Carlberg C, Brandes RP, Seuter S. Sci Rep. 2021 Mar 22;11(1):6518. doi: 10.1038/s41598-021-86032-5. PubMed
Mutant ASXL1 induces age-related expansion of phenotypic hematopoietic stem cells through activation of Akt/mTOR pathway. Fujino T, Goyama S, Sugiura Y, Inoue D, Asada S, Yamasaki S, Matsumoto A, Yamaguchi K, Isobe Y, Tsuchiya A, Shikata S, Sato N, Morinaga H, Fukuyama T, Tanaka Y, Fukushima T, Takeda R, Yamamoto K, Honda H, Nishimura EK, Furukawa Y, Shibata T, Abdel-Wahab O, Suematsu M, Kitamura T. Nat Commun. 2021 Mar 23;12(1):1826. doi: 10.1038/s41467-021-22053-y. PubMed
AWP1 Restrains the Aggressive Behavior of Breast Cancer Cells Induced by TNF-alpha. Kim EY, Kim JE, Choi B, Kweon J, Park SO, Lee HS, Lee EJ, Oh S, Shin HR, Choi H, Kim Y, Chang EJ. Front Oncol. 2021 Mar 18;11:631469. doi: 10.3389/fonc.2021.631469. eCollection 2021. PubMed
The mitochondrial Ca(2+) uptake regulator, MICU1, is involved in cold stress-induced ferroptosis. Nakamura T, Ogawa M, Kojima K, Takayanagi S, Ishihara S, Hattori K, Naguro I, Ichijo H. EMBO Rep. 2021 May 5;22(5):e51532. doi: 10.15252/embr.202051532. Epub 2021 Apr 6. PubMed
EIF5A2 controls ovarian tumor growth and metastasis by promoting epithelial to mesenchymal transition via the TGFbeta pathway. Zhao G, Zhang W, Dong P, Watari H, Guo Y, Pfeffer LM, Tigyi G, Yue J. Cell Biosci. 2021 Apr 7;11(1):70. doi: 10.1186/s13578-021-00578-5. PubMed
Structural variant evolution after telomere crisis. Dewhurst SM, Yao X, Rosiene J, Tian H, Behr J, Bosco N, Takai KK, de Lange T, Imielinski M. Nat Commun. 2021 Apr 7;12(1):2093. doi: 10.1038/s41467-021-21933-7. PubMed
Silencing of LINE-1 retrotransposons is a selective dependency of myeloid leukemia. Gu Z, Liu Y, Zhang Y, Cao H, Lyu J, Wang X, Wylie A, Newkirk SJ, Jones AE, Lee M Jr, Botten GA, Deng M, Dickerson KE, Zhang CC, An W, Abrams JM, Xu J. Nat Genet. 2021 May;53(5):672-682. doi: 10.1038/s41588-021-00829-8. Epub 2021 Apr 8. PubMed
Development of p53 knockout U87MG cell line for unbiased drug delivery testing system using CRISPR-Cas9 and transcriptomic analysis. Kwon AT, Mohri K, Takizawa S, Arakawa T, Takahashi M, Kaczkowski B, Furuno M, Suzuki H, Tagami S, Mukai H, Arner E. J Biotechnol. 2021 May 20;332:72-82. doi: 10.1016/j.jbiotec.2021.03.017. Epub 2021 Apr 6. PubMed
Genome-wide screens uncover KDM2B as a modifier of protein binding to heparan sulfate. Weiss RJ, Spahn PN, Chiang AWT, Liu Q, Li J, Hamill KM, Rother S, Clausen TM, Hoeksema MA, Timm BM, Godula K, Glass CK, Tor Y, Gordts PLSM, Lewis NE, Esko JD. Nat Chem Biol. 2021 Jun;17(6):684-692. doi: 10.1038/s41589-021-00776-9. Epub 2021 Apr 12. PubMed
Bimodal regulation of the PRC2 complex by USP7 underlies tumorigenesis. Su D, Wang W, Hou Y, Wang L, Yi X, Cao C, Wang Y, Gao H, Wang Y, Yang C, Liu B, Chen X, Wu X, Wu J, Yan D, Wei S, Han L, Liu S, Wang Q, Shi L, Shan L. Nucleic Acids Res. 2021 May 7;49(8):4421-4440. doi: 10.1093/nar/gkab209. PubMed
B Cell Receptor-Responsive miR-141 Enhances Epstein-Barr Virus Lytic Cycle via FOXO3 Inhibition. Chen Y, Fachko DN, Ivanov NS, Skalsky RL. mSphere. 2021 Apr 14;6(2). pii: 6/2/e00093-21. doi: 10.1128/mSphere.00093-21. PubMed
ZNF410 represses fetal globin by singular control of CHD4. Vinjamur DS, Yao Q, Cole MA, McGuckin C, Ren C, Zeng J, Hossain M, Luk K, Wolfe SA, Pinello L, Bauer DE. Nat Genet. 2021 May;53(5):719-728. doi: 10.1038/s41588-021-00843-w. Epub 2021 Apr 15. PubMed
Identification of X-chromosomal genes that drive sex differences in embryonic stem cells through a hierarchical CRISPR screening approach. Genolet O, Monaco AA, Dunkel I, Boettcher M, Schulz EG. Genome Biol. 2021 Apr 16;22(1):110. doi: 10.1186/s13059-021-02321-2. PubMed
NSDHL promotes triple-negative breast cancer metastasis through the TGFbeta signaling pathway and cholesterol biosynthesis. Chen M, Zhao Y, Yang X, Zhao Y, Liu Q, Liu Y, Hou Y, Sun H, Jin W. Breast Cancer Res Treat. 2021 Jun;187(2):349-362. doi: 10.1007/s10549-021-06213-8. Epub 2021 Apr 16. PubMed
STING enhances cell death through regulation of reactive oxygen species and DNA damage. Hayman TJ, Baro M, MacNeil T, Phoomak C, Aung TN, Cui W, Leach K, Iyer R, Challa S, Sandoval-Schaefer T, Burtness BA, Rimm DL, Contessa JN. Nat Commun. 2021 Apr 19;12(1):2327. doi: 10.1038/s41467-021-22572-8. PubMed
Selective Modulation of a Pan-Essential Protein as a Therapeutic Strategy in Cancer. Malone CF, Dharia NV, Kugener G, Forman AB, Rothberg MV, Abdusamad M, Gonzalez A, Kuljanin M, Robichaud AL, Conway AS, Dempster JM, Paolella BR, Dumont N, Hovestadt V, Mancias JD, Younger ST, Root DE, Golub TR, Vazquez F, Stegmaier K. Cancer Discov. 2021 Sep;11(9):2282-2299. doi: 10.1158/2159-8290.CD-20-1213. Epub 2021 Apr 21. PubMed
Whole-genome sequencing association analysis of quantitative red blood cell phenotypes: The NHLBI TOPMed program. Hu Y, Stilp AM, McHugh CP, Rao S, Jain D, Zheng X, Lane J, Meric de Bellefon S, Raffield LM, Chen MH, Yanek LR, Wheeler M, Yao Y, Ren C, Broome J, Moon JY, de Vries PS, Hobbs BD, Sun Q, Surendran P, Brody JA, Blackwell TW, Choquet H, Ryan K, Duggirala R, Heard-Costa N, Wang Z, Chami N, Preuss MH, Min N, Ekunwe L, Lange LA, Cushman M, Faraday N, Curran JE, Almasy L, Kundu K, Smith AV, Gabriel S, Rotter JI, Fornage M, Lloyd-Jones DM, Vasan RS, Smith NL, North KE, Boerwinkle E, Becker LC, Lewis JP, Abecasis GR, Hou L, O'Connell JR, Morrison AC, Beaty TH, Kaplan R, Correa A, Blangero J, Jorgenson E, Psaty BM, Kooperberg C, Walton RT, Kleinstiver BP, Tang H, Loos RJF, Soranzo N, Butterworth AS, Nickerson D, Rich SS, Mitchell BD, Johnson AD, Auer PL, Li Y, Mathias RA, Lettre G, Pankratz N, Laurie CC, Laurie CA, Bauer DE, Conomos MP, Reiner AP. Am J Hum Genet. 2021 May 6;108(5):874-893. doi: 10.1016/j.ajhg.2021.04.003. Epub 2021 Apr 21. PubMed
Farnesyl pyrophosphate is a new danger signal inducing acute cell death. Chen J, Zhang X, Li L, Ma X, Yang C, Liu Z, Li C, Fernandez-Cabezudo MJ, Al-Ramadi BK, Wu C, Huang W, Zhang Y, Zhang Y, Liu W. PLoS Biol. 2021 Apr 26;19(4):e3001134. doi: 10.1371/journal.pbio.3001134. eCollection 2021 Apr. PubMed
Sarcomere function activates a p53-dependent DNA damage response that promotes polyploidization and limits in vivo cell engraftment. Pettinato AM, Yoo D, VanOudenhove J, Chen YS, Cohn R, Ladha FA, Yang X, Thakar K, Romano R, Legere N, Meredith E, Robson P, Regnier M, Cotney JL, Murry CE, Hinson JT. Cell Rep. 2021 May 4;35(5):109088. doi: 10.1016/j.celrep.2021.109088. PubMed
Single-nucleotide-level mapping of DNA regulatory elements that control fetal hemoglobin expression. Cheng L, Li Y, Qi Q, Xu P, Feng R, Palmer L, Chen J, Wu R, Yee T, Zhang J, Yao Y, Sharma A, Hardison RC, Weiss MJ, Cheng Y. Nat Genet. 2021 Jun;53(6):869-880. doi: 10.1038/s41588-021-00861-8. Epub 2021 May 6. PubMed
Cell-surface SLC nucleoside transporters and purine levels modulate BRD4-dependent chromatin states. Li KC, Girardi E, Kartnig F, Grosche S, Pemovska T, Bigenzahn JW, Goldmann U, Sedlyarov V, Bensimon A, Schick S, Lin JG, Gurtl B, Reil D, Klavins K, Kubicek S, Sdelci S, Superti-Furga G. Nat Metab. 2021 May;3(5):651-664. doi: 10.1038/s42255-021-00386-8. Epub 2021 May 10. PubMed
The role of sigma 1 receptor in organization of endoplasmic reticulum signaling microdomains. Zhemkov V, Ditlev JA, Lee WR, Wilson M, Liou J, Rosen MK, Bezprozvanny I. Elife. 2021 May 11;10. pii: 65192. doi: 10.7554/eLife.65192. PubMed
Functional mapping of androgen receptor enhancer activity. Huang CF, Lingadahalli S, Morova T, Ozturan D, Hu E, Yu IPL, Linder S, Hoogstraat M, Stelloo S, Sar F, van der Poel H, Altintas UB, Saffarzadeh M, Le Bihan S, McConeghy B, Gokbayrak B, Feng FY, Gleave ME, Bergman AM, Collins C, Hach F, Zwart W, Emberly E, Lack NA. Genome Biol. 2021 May 11;22(1):149. doi: 10.1186/s13059-021-02339-6. PubMed
HIV-based lentiviral vectors: origin and sequence differences. Johnson NM, Alvarado AF, Moffatt TN, Edavettal JM, Swaminathan TA, Braun SE. Mol Ther Methods Clin Dev. 2021 Mar 27;21:451-465. doi: 10.1016/j.omtm.2021.03.018. eCollection 2021 Jun 11. PubMed
TRIM37 prevents formation of condensate-organized ectopic spindle poles to ensure mitotic fidelity. Meitinger F, Kong D, Ohta M, Desai A, Oegema K, Loncarek J. J Cell Biol. 2021 Jul 5;220(7). pii: 212098. doi: 10.1083/jcb.202010180. Epub 2021 May 13. PubMed
Selective Janus kinase inhibition preserves interferon-lambda-mediated antiviral responses. Schnepf D, Crotta S, Thamamongood T, Stanifer M, Polcik L, Ohnemus A, Vier J, Jakob C, Llorian M, Gad HH, Hartmann R, Strobl B, Kirschnek S, Boulant S, Schwemmle M, Wack A, Staeheli P. Sci Immunol. 2021 May 14;6(59). pii: 6/59/eabd5318. doi: 10.1126/sciimmunol.abd5318. PubMed
A virulent Bacillus cereus strain from deep-sea cold seep induces pyroptosis in a manner that involves NLRP3 inflammasome, JNK pathway, and lysosomal rupture. Zhao Y, Jiang S, Zhang J, Guan XL, Sun BG, Sun L. Virulence. 2021 Dec;12(1):1362-1376. doi: 10.1080/21505594.2021.1926649. PubMed
Cargo receptor-assisted endoplasmic reticulum export of pathogenic alpha1-antitrypsin polymers. Ordonez A, Harding HP, Marciniak SJ, Ron D. Cell Rep. 2021 May 18;35(7):109144. doi: 10.1016/j.celrep.2021.109144. PubMed
The metabolite alpha-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8. Zhang JY, Zhou B, Sun RY, Ai YL, Cheng K, Li FN, Wang BR, Liu FJ, Jiang ZH, Wang WJ, Zhou D, Chen HZ, Wu Q. Cell Res. 2021 Sep;31(9):980-997. doi: 10.1038/s41422-021-00506-9. Epub 2021 May 19. PubMed
In vivo adenine base editing of PCSK9 in macaques reduces LDL cholesterol levels. Rothgangl T, Dennis MK, Lin PJC, Oka R, Witzigmann D, Villiger L, Qi W, Hruzova M, Kissling L, Lenggenhager D, Borrelli C, Egli S, Frey N, Bakker N, Walker JA 2nd, Kadina AP, Victorov DV, Pacesa M, Kreutzer S, Kontarakis Z, Moor A, Jinek M, Weissman D, Stoffel M, van Boxtel R, Holden K, Pardi N, Thony B, Haberle J, Tam YK, Semple SC, Schwank G. Nat Biotechnol. 2021 Aug;39(8):949-957. doi: 10.1038/s41587-021-00933-4. Epub 2021 May 19. PubMed
PKD2 deficiency suppresses amino acid biosynthesis in ADPKD by impairing the PERK-TBL2-eIF2a-ATF4 pathway. Zhou X, Xiong H, Lu Y, Geng M, Lu Y, Zhang L, Zhu X. Biochem Biophys Res Commun. 2021 Jul 5;561:73-79. doi: 10.1016/j.bbrc.2021.05.012. Epub 2021 May 18. PubMed
Protein neddylation as a therapeutic target in pulmonary and extrapulmonary small cell carcinomas. Norton JP, Augert A, Eastwood E, Basom R, Rudin CM, MacPherson D. Genes Dev. 2021 Jun;35(11-12):870-887. doi: 10.1101/gad.348316.121. Epub 2021 May 20. PubMed
MPP8 is essential for sustaining self-renewal of ground-state pluripotent stem cells. Muller I, Moroni AS, Shlyueva D, Sahadevan S, Schoof EM, Radzisheuskaya A, Hojfeldt JW, Tatar T, Koche RP, Huang C, Helin K. Nat Commun. 2021 May 24;12(1):3034. doi: 10.1038/s41467-021-23308-4. PubMed

Associated Plasmids
Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal coronaviruses. Rebendenne A, Roy P, Bonaventure B, Chaves Valadao AL, Desmarets L, Rouille Y, Tauziet M, Arnaud-Arnould M, Giovannini D, Lee Y, DeWeirdt P, Hegde M, Garcia de Gracia F, McKellar J, Wencker M, Dubuisson J, Belouzard S, Moncorge O, Doench JG, Goujon C. bioRxiv. 2021 May 21. doi: 10.1101/2021.05.19.444823. PubMed
A BMPR2/YY1 Signaling Axis Is Required for Human Cytomegalovirus Latency in Undifferentiated Myeloid Cells. Poole E, Carlan da Silva MC, Huang C, Perera M, Jackson S, Groves IJ, Wills M, Rana A, Sinclair J. mBio. 2021 Jun 29;12(3):e0022721. doi: 10.1128/mBio.00227-21. Epub 2021 Jun 1. PubMed
Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs. Goujon C, Rebendenne A, Roy P, Bonaventure B, Valadao AC, Desmarets L, Rouille Y, Tauziet M, Arnaud-Arnould M, Giovannini D, Lee Y, DeWeirdt P, Hegde M, de Gracia FG, McKellar J, Wencker M, Dubuisson J, Belouzard S, Moncorge O, Doench J. Res Sq. 2021 May 27. doi: 10.21203/rs.3.rs-555275/v1. PubMed
The WAVE complex associates with sites of saddle membrane curvature. Pipathsouk A, Brunetti RM, Town JP, Graziano BR, Breuer A, Pellett PA, Marchuk K, Tran NT, Krummel MF, Stamou D, Weiner OD. J Cell Biol. 2021 Aug 2;220(8). pii: 212260. doi: 10.1083/jcb.202003086. Epub 2021 Jun 7. PubMed
A cell-free assay implicates a role of sphingomyelin and cholesterol in STING phosphorylation. Takahashi K, Niki T, Ogawa E, Fumika K, Nishioka Y, Sawa M, Arai H, Mukai K, Taguchi T. Sci Rep. 2021 Jun 7;11(1):11996. doi: 10.1038/s41598-021-91562-z. PubMed
SMCHD1's ubiquitin-like domain is required for N-terminal dimerization and chromatin localization. Gurzau AD, Horne CR, Mok YF, Iminitoff M, Willson TA, Young SN, Blewitt ME, Murphy JM. Biochem J. 2021 Jul 16;478(13):2555-2569. doi: 10.1042/BCJ20210278. PubMed
HGK promotes metastatic dissemination in prostate cancer. Garcia-Garcia S, Rodrigo-Faus M, Fonseca N, Manzano S, Gyorffy B, Ocana A, Bragado P, Porras A, Gutierrez-Uzquiza A. Sci Rep. 2021 Jun 10;11(1):12287. doi: 10.1038/s41598-021-91292-2. PubMed
Single-cell lineage tracing of metastatic cancer reveals selection of hybrid EMT states. Simeonov KP, Byrns CN, Clark ML, Norgard RJ, Martin B, Stanger BZ, Shendure J, McKenna A, Lengner CJ. Cancer Cell. 2021 Aug 9;39(8):1150-1162.e9. doi: 10.1016/j.ccell.2021.05.005. Epub 2021 Jun 10. PubMed

Associated Plasmids
Inactivation of Latent HIV-1 Proviral DNA Using Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Treatment and the Assessment of Off-Target Effects. Xu Y, Peng X, Zheng Y, Jin C, Lu X, Han D, Fu H, Chen C, Wu N. Front Microbiol. 2021 May 26;12:629153. doi: 10.3389/fmicb.2021.629153. eCollection 2021. PubMed
A PGE2-MEF2A axis enables context-dependent control of inflammatory gene expression. Cilenti F, Barbiera G, Caronni N, Iodice D, Montaldo E, Barresi S, Lusito E, Cuzzola V, Vittoria FM, Mezzanzanica L, Miotto P, Di Lucia P, Lazarevic D, Cirillo DM, Iannacone M, Genua M, Ostuni R. Immunity. 2021 Aug 10;54(8):1665-1682.e14. doi: 10.1016/j.immuni.2021.05.016. Epub 2021 Jun 14. PubMed
CRISPR mediated targeting of DUX4 distal regulatory element represses DUX4 target genes dysregulated in Facioscapulohumeral muscular dystrophy. Das S, Chadwick BP. Sci Rep. 2021 Jun 15;11(1):12598. doi: 10.1038/s41598-021-92096-0. PubMed
Genome-scale CRISPR-Cas9 screen of Wnt/beta-catenin signaling identifies therapeutic targets for colorectal cancer. Wan C, Mahara S, Sun C, Doan A, Chua HK, Xu D, Bian J, Li Y, Zhu D, Sooraj D, Cierpicki T, Grembecka J, Firestein R. Sci Adv. 2021 May 19;7(21). pii: 7/21/eabf2567. doi: 10.1126/sciadv.abf2567. Print 2021 May. PubMed
Monocarboxylate transporter antagonism reveals metabolic vulnerabilities of viral-driven lymphomas. Bonglack EN, Messinger JE, Cable JM, Ch'ng J, Parnell KM, Reinoso-Vizcaino NM, Barry AP, Russell VS, Dave SS, Christofk HR, Luftig MA. Proc Natl Acad Sci U S A. 2021 Jun 22;118(25). pii: 2022495118. doi: 10.1073/pnas.2022495118. PubMed
Pharmacologic modulation of RNA splicing enhances anti-tumor immunity. Lu SX, De Neef E, Thomas JD, Sabio E, Rousseau B, Gigoux M, Knorr DA, Greenbaum B, Elhanati Y, Hogg SJ, Chow A, Ghosh A, Xie A, Zamarin D, Cui D, Erickson C, Singer M, Cho H, Wang E, Lu B, Durham BH, Shah H, Chowell D, Gabel AM, Shen Y, Liu J, Jin J, Rhodes MC, Taylor RE, Molina H, Wolchok JD, Merghoub T, Diaz LA Jr, Abdel-Wahab O, Bradley RK. Cell. 2021 Jul 22;184(15):4032-4047.e31. doi: 10.1016/j.cell.2021.05.038. Epub 2021 Jun 24. PubMed
A first-in-class Polymerase Theta Inhibitor selectively targets Homologous-Recombination-Deficient Tumors. Zhou J, Gelot C, Pantelidou C, Li A, Yucel H, Davis RE, Farkkila A, Kochupurakkal B, Syed A, Shapiro GI, Tainer JA, Blagg BSJ, Ceccaldi R, D'Andrea AD. Nat Cancer. 2021 Jun;2(6):598-610. doi: 10.1038/s43018-021-00203-x. Epub 2021 Jun 17. PubMed
The PD-L1/PD-1 Axis Blocks Neutrophil Cytotoxicity in Cancer. Yajuk O, Baron M, Toker S, Zelter T, Fainsod-Levi T, Granot Z. Cells. 2021 Jun 15;10(6). pii: cells10061510. doi: 10.3390/cells10061510. PubMed
HNRNPL Circularizes ARHGAP35 to Produce an Oncogenic Protein. Li Y, Chen B, Zhao J, Li Q, Chen S, Guo T, Li Y, Lai H, Chen Z, Meng Z, Guo W, He X, Huang S. Adv Sci (Weinh). 2021 May 1;8(13):2001701. doi: 10.1002/advs.202001701. eCollection 2021 Jul. PubMed
Glycerol-3-phosphate biosynthesis regenerates cytosolic NAD(+) to alleviate mitochondrial disease. Liu S, Fu S, Wang G, Cao Y, Li L, Li X, Yang J, Li N, Shan Y, Cao Y, Ma Y, Dong M, Liu Q, Jiang H. Cell Metab. 2021 Jul 8. pii: S1550-4131(21)00283-7. doi: 10.1016/j.cmet.2021.06.013. PubMed
Machine learning approach for discrimination of genotypes based on bright-field cellular images. Suzuki G, Saito Y, Seki M, Evans-Yamamoto D, Negishi M, Kakoi K, Kawai H, Landry CR, Yachie N, Mitsuyama T. NPJ Syst Biol Appl. 2021 Jul 21;7(1):31. doi: 10.1038/s41540-021-00190-w. PubMed
Dual-AAV delivering split prime editor system for in vivo genome editing. Zhi S, Chen Y, Wu G, Wen J, Wu J, Liu Q, Li Y, Kang R, Hu S, Wang J, Liang P, Huang J. Mol Ther. 2021 Jul 21. pii: S1525-0016(21)00365-8. doi: 10.1016/j.ymthe.2021.07.011. PubMed
Human cell transformation by combined lineage conversion and oncogene expression. Sahu B, Pihlajamaa P, Zhang K, Palin K, Ahonen S, Cervera A, Ristimaki A, Aaltonen LA, Hautaniemi S, Taipale J. Oncogene. 2021 Sep;40(36):5533-5547. doi: 10.1038/s41388-021-01940-0. Epub 2021 Jul 23. PubMed
An autoimmune disease risk variant: A trans master regulatory effect mediated by IRF1 under immune stimulation? Brandt M, Kim-Hellmuth S, Ziosi M, Gokden A, Wolman A, Lam N, Recinos Y, Daniloski Z, Morris JA, Hornung V, Schumacher J, Lappalainen T. PLoS Genet. 2021 Jul 27;17(7):e1009684. doi: 10.1371/journal.pgen.1009684. eCollection 2021 Jul. PubMed
Metabolically controlled histone H4K5 acylation/acetylation ratio drives BRD4 genomic distribution. Gao M, Wang J, Rousseaux S, Tan M, Pan L, Peng L, Wang S, Xu W, Ren J, Liu Y, Spinck M, Barral S, Wang T, Chuffart F, Bourova-Flin E, Puthier D, Curtet S, Bargier L, Cheng Z, Neumann H, Li J, Zhao Y, Mi JQ, Khochbin S. Cell Rep. 2021 Jul 27;36(4):109460. doi: 10.1016/j.celrep.2021.109460. PubMed
NS5-independent Ablation of STAT2 by Zika virus to antagonize interferon signalling. Shu J, Ma X, Zhang Y, Zou J, Yuan Z, Yi Z. Emerg Microbes Infect. 2021 Dec;10(1):1609-1625. doi: 10.1080/22221751.2021.1964384. PubMed
A UVB-responsive common variant at chromosome band 7p21.1 confers tanning response and melanoma risk via regulation of the aryl hydrocarbon receptor, AHR. Xu M, Mehl L, Zhang T, Thakur R, Sowards H, Myers T, Jessop L, Chesi A, Johnson ME, Wells AD, Michael HT, Bunda P, Jones K, Higson H, Hennessey RC, Jermusyk A, Kovacs MA, Landi MT, Iles MM, Goldstein AM, Choi J, Chanock SJ, Grant SFA, Chari R, Merlino G, Law MH, Brown KM. Am J Hum Genet. 2021 Sep 2;108(9):1611-1630. doi: 10.1016/j.ajhg.2021.07.002. Epub 2021 Aug 2. PubMed
Targeting leukemia-specific dependence on the de novo purine synthesis pathway. Yamauchi T, Miyawaki K, Semba Y, Takahashi M, Izumi Y, Nogami J, Nakao F, Sugio T, Sasaki K, Pinello L, Bauer DE, Bamba T, Akashi K, Maeda T. Leukemia. 2021 Aug 3. pii: 10.1038/s41375-021-01369-0. doi: 10.1038/s41375-021-01369-0. PubMed
Targeting BRF2 in Cancer Using Repurposed Drugs. Rashidieh B, Molakarimi M, Mohseni A, Tria SM, Truong H, Srihari S, Adams RC, Jones M, Duijf PHG, Kalimutho M, Khanna KK. Cancers (Basel). 2021 Jul 27;13(15). pii: cancers13153778. doi: 10.3390/cancers13153778. PubMed
Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer. Vichas A, Riley AK, Nkinsi NT, Kamlapurkar S, Parrish PCR, Lo A, Duke F, Chen J, Fung I, Watson J, Rees M, Gabel AM, Thomas JD, Bradley RK, Lee JK, Hatch EM, Baine MK, Rekhtman N, Ladanyi M, Piccioni F, Berger AH. Nat Commun. 2021 Aug 9;12(1):4789. doi: 10.1038/s41467-021-24841-y. PubMed
Chemical-genetic CRISPR-Cas9 screens in human cells using a pathway-specific library. Hundley FV, Toczyski DP. STAR Protoc. 2021 Jul 28;2(3):100685. doi: 10.1016/j.xpro.2021.100685. eCollection 2021 Sep 17. PubMed
LINC00882 Plays a Tumor-promoter Role in Colorectal Cancer by Targeting miR-3619-5p to Up-regulate CTNNB1. Song B, Li H, Guo S, Yang T, Li L, Cao L, Wang J. Arch Med Res. 2022 Jan;53(1):29-36. doi: 10.1016/j.arcmed.2021.06.001. Epub 2021 Aug 13. PubMed
ELMO1 signaling is a promoter of osteoclast function and bone loss. Arandjelovic S, Perry JSA, Zhou M, Ceroi A, Smirnov I, Walk SF, Shankman LS, Cambre I, Onengut-Gumuscu S, Elewaut D, Conrads TP, Ravichandran KS. Nat Commun. 2021 Aug 17;12(1):4974. doi: 10.1038/s41467-021-25239-6. PubMed
Systematic functional interrogation of human pseudogenes using CRISPRi. Sun M, Wang Y, Zheng C, Wei Y, Hou J, Zhang P, He W, Lv X, Ding Y, Liang H, Hon CC, Chen X, Xu H, Chen Y. Genome Biol. 2021 Aug 23;22(1):240. doi: 10.1186/s13059-021-02464-2. PubMed
Intrinsic activation of beta-catenin signaling by CRISPR/Cas9-mediated exon skipping contributes to immune evasion in hepatocellular carcinoma. Akasu M, Shimada S, Kabashima A, Akiyama Y, Shimokawa M, Akahoshi K, Kudo A, Yamaoka S, Tanabe M, Tanaka S. Sci Rep. 2021 Aug 24;11(1):16732. doi: 10.1038/s41598-021-96167-0. PubMed
Functional genomics atlas of synovial fibroblasts defining rheumatoid arthritis heritability. Ge X, Frank-Bertoncelj M, Klein K, McGovern A, Kuret T, Houtman M, Burja B, Micheroli R, Shi C, Marks M, Filer A, Buckley CD, Orozco G, Distler O, Morris AP, Martin P, Eyre S, Ospelt C. Genome Biol. 2021 Aug 25;22(1):247. doi: 10.1186/s13059-021-02460-6. PubMed
Predicting base editing outcomes with an attention-based deep learning algorithm trained on high-throughput target library screens. Marquart KF, Allam A, Janjuha S, Sintsova A, Villiger L, Frey N, Krauthammer M, Schwank G. Nat Commun. 2021 Aug 25;12(1):5114. doi: 10.1038/s41467-021-25375-z. PubMed
Discovery of synthetic lethal and tumor suppressor paralog pairs in the human genome. Parrish PCR, Thomas JD, Gabel AM, Kamlapurkar S, Bradley RK, Berger AH. Cell Rep. 2021 Aug 31;36(9):109597. doi: 10.1016/j.celrep.2021.109597. PubMed

Associated Plasmids
A novel PLK1 inhibitor onvansertib effectively sensitizes MYC-driven medulloblastoma to radiotherapy. Wang D, Veo B, Pierce A, Fosmire S, Madhavan K, Balakrishnan I, Donson A, Alimova I, Sullivan KD, Joshi M, Erlander M, Ridinger M, Foreman NK, Venkataraman S, Vibhakar R. Neuro Oncol. 2022 Mar 12;24(3):414-426. doi: 10.1093/neuonc/noab207. PubMed
AAGAB is an assembly chaperone regulating AP1 and AP2 clathrin adaptors. Wan C, Crisman L, Wang B, Tian Y, Wang S, Yang R, Datta I, Nomura T, Li S, Yu H, Yin Q, Shen J. J Cell Sci. 2021 Oct 1;134(19). pii: 272394. doi: 10.1242/jcs.258587. Epub 2021 Oct 5. PubMed
Using CRISPR Interference as a Therapeutic Approach to Treat TGFbeta2-Induced Ocular Hypertension and Glaucoma. Rayana NP, Sugali CK, Dai J, Peng M, Liu S, Zhang Y, Wan J, Mao W. Invest Ophthalmol Vis Sci. 2021 Sep 2;62(12):7. doi: 10.1167/iovs.62.12.7. PubMed
Host phospholipid peroxidation fuels ExoU-dependent cell necrosis and supports Pseudomonas aeruginosa-driven pathology. Bagayoko S, Leon-Icaza SA, Pinilla M, Hessel A, Santoni K, Pericat D, Bordignon PJ, Moreau F, Eren E, Boyance A, Naser E, Lefevre L, Berrone C, Iakobachvili N, Metais A, Rombouts Y, Lugo-Villarino G, Coste A, Attree I, Frank DW, Clevers H, Peters PJ, Cougoule C, Planes R, Meunier E. PLoS Pathog. 2021 Sep 13;17(9):e1009927. doi: 10.1371/journal.ppat.1009927. eCollection 2021 Sep. PubMed
Broad antiviral and anti-inflammatory efficacy of nafamostat against SARS-CoV-2 and seasonal coronaviruses in primary human bronchiolar epithelia. Niemeyer BF, Miller CM, Ledesma-Feliciano C, Morrison JH, Jimenez-Valdes R, Clifton C, Poeschla EM, Benam KH. Nano Sel. 2022 Feb;3(2):437-449. doi: 10.1002/nano.202100123. Epub 2021 Jun 30. PubMed
ATF4-mediated transcriptional regulation protects against beta-cell loss during endoplasmic reticulum stress in a mouse model. Kitakaze K, Oyadomari M, Zhang J, Hamada Y, Takenouchi Y, Tsuboi K, Inagaki M, Tachikawa M, Fujitani Y, Okamoto Y, Oyadomari S. Mol Metab. 2021 Dec;54:101338. doi: 10.1016/j.molmet.2021.101338. Epub 2021 Sep 20. PubMed
Sphingosine 1-phosphate receptor type 2 positively regulates interleukin (IL)-4/IL-13-induced STAT6 phosphorylation. Okamoto Y, Kitakaze K, Takenouchi Y, Yamamoto S, Ishimaru H, Tsuboi K. Cell Signal. 2021 Dec;88:110156. doi: 10.1016/j.cellsig.2021.110156. Epub 2021 Sep 28. PubMed
Inhibition of the ubiquitin-proteasome system by an NQO1-activatable compound. Giovannucci TA, Salomons FA, Haraldsson M, Elfman LHM, Wickstrom M, Young P, Lundback T, Eirich J, Altun M, Jafari R, Gustavsson AL, Johnsen JI, Dantuma NP. Cell Death Dis. 2021 Oct 6;12(10):914. doi: 10.1038/s41419-021-04191-9. PubMed
Replacing the SpCas9 HNH domain by deaminases generates compact base editors with an alternative targeting scope. Villiger L, Schmidheini L, Mathis N, Rothgangl T, Marquart K, Schwank G. Mol Ther Nucleic Acids. 2021 Aug 26;26:502-510. doi: 10.1016/j.omtn.2021.08.025. eCollection 2021 Dec 3. PubMed

Associated Plasmids
Translational repression of NMD targets by GIGYF2 and EIF4E2. Zinshteyn B, Sinha NK, Enam SU, Koleske B, Green R. PLoS Genet. 2021 Oct 19;17(10):e1009813. doi: 10.1371/journal.pgen.1009813. eCollection 2021 Oct. PubMed
Retinoic acid rewires the adrenergic core regulatory circuitry of childhood neuroblastoma. Zimmerman MW, Durbin AD, He S, Oppel F, Shi H, Tao T, Li Z, Berezovskaya A, Liu Y, Zhang J, Young RA, Abraham BJ, Look AT. Sci Adv. 2021 Oct 22;7(43):eabe0834. doi: 10.1126/sciadv.abe0834. Epub 2021 Oct 20. PubMed
Development of a selective fluorescence-based enzyme assay for glycerophosphodiesterase family members GDE4 and GDE7. Kitakaze K, Tsuboi K, Tsuda M, Takenouchi Y, Ishimaru H, Okamoto Y. J Lipid Res. 2021;62:100141. doi: 10.1016/j.jlr.2021.100141. Epub 2021 Oct 18. PubMed
Dnmt3a-mutated clonal hematopoiesis promotes osteoporosis. Kim PG, Niroula A, Shkolnik V, McConkey M, Lin AE, Slabicki M, Kemp JP, Bick A, Gibson CJ, Griffin G, Sekar A, Brooks DJ, Wong WJ, Cohen DN, Uddin MM, Shin WJ, Pirruccello J, Tsai JM, Agrawal M, Kiel DP, Bouxsein ML, Richards JB, Evans DM, Wein MN, Charles JF, Jaiswal S, Natarajan P, Ebert BL. J Exp Med. 2021 Dec 6;218(12). pii: 212724. doi: 10.1084/jem.20211872. Epub 2021 Oct 26. PubMed
Co-occurrence of BAP1 and SF3B1 mutations in uveal melanoma induces cellular senescence. Yu L, Zhou D, Zhang G, Ren Z, Luo X, Liu P, Plouffe SW, Meng Z, Moroishi T, Li Y, Zhang Y, Brown JH, Liu S, Guan KL. Mol Oncol. 2022 Feb;16(3):607-629. doi: 10.1002/1878-0261.13128. Epub 2021 Nov 12. PubMed
Target Identification of Small Molecules Using Large-Scale CRISPR-Cas Mutagenesis Scanning of Essential Genes. Kwanten B, Neggers JE, Daelemans D. Methods Mol Biol. 2022;2377:43-67. doi: 10.1007/978-1-0716-1720-5_3. PubMed
Combined DNA Damage Repair Interference and Ion Beam Therapy: Development, Benchmark, and Clinical Implications of a Mechanistic Biological Model. Liew H, Meister S, Mein S, Tessonnier T, Kopp B, Held T, Haberer T, Abdollahi A, Debus J, Dokic I, Mairani A. Int J Radiat Oncol Biol Phys. 2022 Mar 1;112(3):802-817. doi: 10.1016/j.ijrobp.2021.09.048. Epub 2021 Oct 25. PubMed
Temporal controls over inter-areal cortical projection neuron fate diversity. Klingler E, Tomasello U, Prados J, Kebschull JM, Contestabile A, Galinanes GL, Fievre S, Santinha A, Platt R, Huber D, Dayer A, Bellone C, Jabaudon D. Nature. 2021 Nov;599(7885):453-457. doi: 10.1038/s41586-021-04048-3. Epub 2021 Nov 9. PubMed
The specificity of the malarial VAR2CSA protein for chondroitin sulfate depends on 4-O-sulfation and ligand accessibility. Spliid CB, Toledo AG, Sanderson P, Mao Y, Gatto F, Gustavsson T, Choudhary S, Saldanha AL, Vogelsang RP, Gogenur I, Theander TG, Leach FE 3rd, Amster IJ, Esko JD, Salanti A, Clausen TM. J Biol Chem. 2021 Dec;297(6):101391. doi: 10.1016/j.jbc.2021.101391. Epub 2021 Nov 8. PubMed
Substrate Stiffness Drives Epithelial to Mesenchymal Transition and Proliferation through the NEAT1-Wnt/beta-Catenin Pathway in Liver Cancer. Xu X, Zhang Y, Wang X, Li S, Tang L. Int J Mol Sci. 2021 Nov 8;22(21). pii: ijms222112066. doi: 10.3390/ijms222112066. PubMed
Genome-scale CRISPR-Cas9 knockout screening in hepatocellular carcinoma with lenvatinib resistance. Lu Y, Shen H, Huang W, He S, Chen J, Zhang D, Shen Y, Sun Y. Cell Death Discov. 2021 Nov 18;7(1):359. doi: 10.1038/s41420-021-00747-y. PubMed
H3K27ac HiChIP in prostate cell lines identifies risk genes for prostate cancer susceptibility. Giambartolomei C, Seo JH, Schwarz T, Freund MK, Johnson RD, Spisak S, Baca SC, Gusev A, Mancuso N, Pasaniuc B, Freedman ML. Am J Hum Genet. 2021 Dec 2;108(12):2284-2300. doi: 10.1016/j.ajhg.2021.11.007. Epub 2021 Nov 24. PubMed
Genetically edited hepatic cells expressing the NTCP-S267F variant are resistant to hepatitis B virus infection. Uchida T, Park SB, Inuzuka T, Zhang M, Allen JN, Chayama K, Liang TJ. Mol Ther Methods Clin Dev. 2021 Nov 10;23:597-605. doi: 10.1016/j.omtm.2021.11.002. eCollection 2021 Dec 10. PubMed
Low NCOR2 levels in multiple myeloma patients drive multidrug resistance via MYC upregulation. Mori T, Verma R, Nakamoto-Matsubara R, Siu KT, Panaroni C, Fulzele KS, Mukaihara K, Onyewadume C, Maebius A, Kato H, Wong LP, Sadreyev RI, Scadden DT, Raje NS. Blood Cancer J. 2021 Dec 4;11(12):194. doi: 10.1038/s41408-021-00589-y. PubMed
piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition. Wolff JH, Haldrup J, Thomsen EA, Andersen S, Mikkelsen JG. Front Genome Ed. 2021 Nov 12;3:786893. doi: 10.3389/fgeed.2021.786893. eCollection 2021. PubMed

Associated Plasmids
A genome-wide CRISPR/Cas9 gene knockout screen identifies immunoglobulin superfamily DCC subclass member 4 as a key host factor that promotes influenza virus endocytosis. Song Y, Huang H, Hu Y, Zhang J, Li F, Yin X, Shi J, Li Y, Li C, Zhao D, Chen H. PLoS Pathog. 2021 Dec 6;17(12):e1010141. doi: 10.1371/journal.ppat.1010141. eCollection 2021 Dec. PubMed
Single-cell characterization of CRISPR-modified transcript isoforms with nanopore sequencing. Kim HS, Grimes SM, Hooker AC, Lau BT, Ji HP. Genome Biol. 2021 Dec 6;22(1):331. doi: 10.1186/s13059-021-02554-1. PubMed
A predominant enhancer co-amplified with the SOX2 oncogene is necessary and sufficient for its expression in squamous cancer. Liu Y, Wu Z, Zhou J, Ramadurai DKA, Mortenson KL, Aguilera-Jimenez E, Yan Y, Yang X, Taylor AM, Varley KE, Gertz J, Choi PS, Cherniack AD, Chen X, Bass AJ, Bailey SD, Zhang X. Nat Commun. 2021 Dec 8;12(1):7139. doi: 10.1038/s41467-021-27055-4. PubMed
Structural organization of erythrocyte membrane microdomains and their relation with malaria susceptibility. Olivieri A, Lee RS, Fratini F, Keutcha C, Chaand M, Mangano V, Celani F, Mochi S, Birago C, Paone S, Grasso F, Tirelli V, Falchi M, Shabani E, Bertoncini S, Sirima BS, Pizzi E, Modiano D, Duraisingh MT, Ponzi M. Commun Biol. 2021 Dec 8;4(1):1375. doi: 10.1038/s42003-021-02900-w. PubMed
Discovery of novel furanylbenzamide inhibitors that target oncogenic tyrosine phosphatase SHP2 in leukemia cells. Raveendra-Panickar D, Finlay D, Layng FI, Lambert LJ, Celeridad M, Zhao M, Barbosa K, De Backer LJS, Kwong E, Gosalia P, Rodiles S, Holleran J, Ardecky R, Grotegut S, Olson S, Hutchinson JH, Pasquale EB, Vuori K, Deshpande AJ, Cosford NDP, Tautz L. J Biol Chem. 2022 Jan;298(1):101477. doi: 10.1016/j.jbc.2021.101477. Epub 2021 Dec 10. PubMed
BoxCar and shotgun proteomic analyses reveal molecular networks regulated by UBR5 in prostate cancer. Yan Y, Zhou B, Lee YJ, You S, Freeman MR, Yang W. Proteomics. 2022 Feb;22(4):e2100172. doi: 10.1002/pmic.202100172. Epub 2021 Dec 23. PubMed
Pharmacological Activation of cGAS for Cancer Immunotherapy. Garland KM, Rosch JC, Carson CS, Wang-Bishop L, Hanna A, Sevimli S, Van Kaer C, Balko JM, Ascano M, Wilson JT. Front Immunol. 2021 Nov 26;12:753472. doi: 10.3389/fimmu.2021.753472. eCollection 2021. PubMed
Nuclear pore protein NUP210 depletion suppresses metastasis through heterochromatin-mediated disruption of tumor cell mechanical response. Amin R, Shukla A, Zhu JJ, Kim S, Wang P, Tian SZ, Tran AD, Paul D, Cappell SD, Burkett S, Liu H, Lee MP, Kruhlak MJ, Dwyer JE, Simpson RM, Hager GL, Ruan Y, Hunter KW. Nat Commun. 2021 Dec 13;12(1):7216. doi: 10.1038/s41467-021-27451-w. PubMed
An In Vivo CRISPR Screen Identifies Stepwise Genetic Dependencies of Metastatic Progression. Scheidmann MC, Castro-Giner F, Strittmatter K, Krol I, Paasinen-Sohns A, Scherrer R, Donato C, Gkountela S, Szczerba BM, Diamantopoulou Z, Muenst S, Vlajnic T, Kunz L, Vetter M, Rochlitz C, Taylor V, Giachino C, Schroeder T, Platt RJ, Aceto N. Cancer Res. 2022 Feb 15;82(4):681-694. doi: 10.1158/0008-5472.CAN-21-3908. PubMed
Unexpected gene activation following CRISPR-Cas9-mediated genome editing. Manjon AG, Linder S, Teunissen H, Friskes A, Zwart W, de Wit E, Medema RH. EMBO Rep. 2022 Feb 3;23(2):e53902. doi: 10.15252/embr.202153902. Epub 2021 Dec 20. PubMed
VLDLR and ApoER2 are receptors for multiple alphaviruses. Clark LE, Clark SA, Lin C, Liu J, Coscia A, Nabel KG, Yang P, Neel DV, Lee H, Brusic V, Stryapunina I, Plante KS, Ahmed AA, Catteruccia F, Young-Pearse TL, Chiu IM, Llopis PM, Weaver SC, Abraham J. Nature. 2022 Feb;602(7897):475-480. doi: 10.1038/s41586-021-04326-0. Epub 2021 Dec 20. PubMed
GCN2 kinase activation by ATP-competitive kinase inhibitors. Tang CP, Clark O, Ferrarone JR, Campos C, Lalani AS, Chodera JD, Intlekofer AM, Elemento O, Mellinghoff IK. Nat Chem Biol. 2022 Feb;18(2):207-215. doi: 10.1038/s41589-021-00947-8. Epub 2021 Dec 23. PubMed
A beta-Catenin-TCF-Sensitive Locus Control Region Mediates GUCY2C Ligand Loss in Colorectal Cancer. Rappaport JA, Entezari AA, Caspi A, Caksa S, Jhaveri AV, Stanek TJ, Ertel A, Kupper J, Fortina PM, McMahon SB, Jaynes JB, Snook AE, Waldman SA. Cell Mol Gastroenterol Hepatol. 2022;13(4):1276-1296. doi: 10.1016/j.jcmgh.2021.12.014. Epub 2021 Dec 22. PubMed
A one-step platform for screening high-efficient and minimal off-target CRISPR/Cas13 crRNAs to eradicate SARS-CoV-2 virus for treatment of COVID-19 patients. Li PY, Li SQ, Gao SG, Dong DY. Med Hypotheses. 2022 Feb;159:110754. doi: 10.1016/j.mehy.2021.110754. Epub 2022 Jan 4. PubMed
Lysoptosis is an evolutionarily conserved cell death pathway moderated by intracellular serpins. Luke CJ, Markovina S, Good M, Wight IE, Thomas BJ, Linneman JM, Lanik WE, Koroleva O, Coffman MR, Miedel MT, Gong Q, Andress A, Campos Guerrero M, Wang S, Chen L, Beatty WL, Hausmann KN, White FV, Fitzpatrick JAJ, Orvedahl A, Pak SC, Silverman GA. Commun Biol. 2022 Jan 12;5(1):47. doi: 10.1038/s42003-021-02953-x. PubMed
The Hippo pathway kinases LATS1 and LATS2 attenuate cellular responses to heavy metals through phosphorylating MTF1. Han H, Nakaoka HJ, Hofmann L, Zhou JJ, Yu C, Zeng L, Nan J, Seo G, Vargas RE, Yang B, Qi R, Bardwell L, Fishman DA, Cho KWY, Huang L, Luo R, Warrior R, Wang W. Nat Cell Biol. 2022 Jan;24(1):74-87. doi: 10.1038/s41556-021-00813-8. Epub 2022 Jan 13. PubMed
Disruption of actin dynamics induces autophagy of the eukaryotic chaperonin TRiC/CCT. Date Y, Matsuura A, Itakura E. Cell Death Discov. 2022 Jan 25;8(1):37. doi: 10.1038/s41420-022-00828-6. PubMed
Receptor-interacting protein kinase 2 (RIPK2) stabilizes c-Myc and is a therapeutic target in prostate cancer metastasis. Yan Y, Zhou B, Qian C, Vasquez A, Kamra M, Chatterjee A, Lee YJ, Yuan X, Ellis L, Di Vizio D, Posadas EM, Kyprianou N, Knudsen BS, Shah K, Murali R, Gertych A, You S, Freeman MR, Yang W. Nat Commun. 2022 Feb 3;13(1):669. doi: 10.1038/s41467-022-28340-6. PubMed
Selective multi-kinase inhibition sensitizes mesenchymal pancreatic cancer to immune checkpoint blockade by remodeling the tumor microenvironment. Falcomata C, Barthel S, Widholz SA, Schneeweis C, Montero JJ, Toska A, Mir J, Kaltenbacher T, Heetmeyer J, Swietlik JJ, Cheng JY, Teodorescu B, Reichert O, Schmitt C, Grabichler K, Coluccio A, Boniolo F, Veltkamp C, Zukowska M, Vargas AA, Paik WH, Jesinghaus M, Steiger K, Maresch R, Ollinger R, Ammon T, Baranov O, Robles MS, Rechenberger J, Kuster B, Meissner F, Reichert M, Flossdorf M, Rad R, Schmidt-Supprian M, Schneider G, Saur D. Nat Cancer. 2022 Mar;3(3):318-336. doi: 10.1038/s43018-021-00326-1. Epub 2022 Jan 31. PubMed
Identification of an endoplasmic reticulum proteostasis modulator that enhances insulin production in pancreatic beta cells. Miyake M, Sobajima M, Kurahashi K, Shigenaga A, Denda M, Otaka A, Saio T, Sakane N, Kosako H, Oyadomari S. Cell Chem Biol. 2022 Jun 16;29(6):996-1009.e9. doi: 10.1016/j.chembiol.2022.01.002. Epub 2022 Feb 9. PubMed
METTL16 exerts an m(6)A-independent function to facilitate translation and tumorigenesis. Su R, Dong L, Li Y, Gao M, He PC, Liu W, Wei J, Zhao Z, Gao L, Han L, Deng X, Li C, Prince E, Tan B, Qing Y, Qin X, Shen C, Xue M, Zhou K, Chen Z, Xue J, Li W, Qin H, Wu X, Sun M, Nam Y, Chen CW, Huang W, Horne D, Rosen ST, He C, Chen J. Nat Cell Biol. 2022 Feb;24(2):205-216. doi: 10.1038/s41556-021-00835-2. Epub 2022 Feb 10. PubMed
CRISPR and biochemical screens identify MAZ as a cofactor in CTCF-mediated insulation at Hox clusters. Ortabozkoyun H, Huang PY, Cho H, Narendra V, LeRoy G, Gonzalez-Buendia E, Skok JA, Tsirigos A, Mazzoni EO, Reinberg D. Nat Genet. 2022 Feb;54(2):202-212. doi: 10.1038/s41588-021-01008-5. Epub 2022 Feb 10. PubMed
p53 Promotes Cytokine Expression in Melanoma to Regulate Drug Resistance and Migration. Pandya P, Kublo L, Stewart-Ornstein J. Cells. 2022 Jan 25;11(3). pii: cells11030405. doi: 10.3390/cells11030405. PubMed
Combinatorial Approach of Binary Colloidal Crystals and CRISPR Activation to Improve Induced Pluripotent Stem Cell Differentiation into Neurons. Urrutia-Cabrera D, Hsiang-Chi Liou R, Lin J, Shi Y, Liu K, Hung SSC, Hewitt AW, Wang PY, Ching-Bong Wong R. ACS Appl Mater Interfaces. 2022 Feb 23;14(7):8669-8679. doi: 10.1021/acsami.1c17975. Epub 2022 Feb 15. PubMed
Transcriptome-wide association study of coronary artery disease identifies novel susceptibility genes. Li L, Chen Z, von Scheidt M, Li S, Steiner A, Guldener U, Koplev S, Ma A, Hao K, Pan C, Lusis AJ, Pang S, Kessler T, Ermel R, Sukhavasi K, Ruusalepp A, Gagneur J, Erdmann J, Kovacic JC, Bjorkegren JLM, Schunkert H. Basic Res Cardiol. 2022 Feb 17;117(1):6. doi: 10.1007/s00395-022-00917-8. PubMed
RB1 loss overrides PARP inhibitor sensitivity driven by RNASEH2B loss in prostate cancer. Miao C, Tsujino T, Takai T, Gui F, Tsutsumi T, Sztupinszki Z, Wang Z, Azuma H, Szallasi Z, Mouw KW, Zou L, Kibel AS, Jia L. Sci Adv. 2022 Feb 18;8(7):eabl9794. doi: 10.1126/sciadv.abl9794. Epub 2022 Feb 18. PubMed
APP and DYRK1A regulate axonal and synaptic vesicle protein networks and mediate Alzheimer's pathology in trisomy 21 neurons. Wu CI, Vinton EA, Pearse RV 2nd, Heo K, Aylward AJ, Hsieh YC, Bi Y, Adeleye S, Fancher S, Duong DM, Seyfried NT, Schwarz TL, Young-Pearse TL. Mol Psychiatry. 2022 Apr;27(4):1970-1989. doi: 10.1038/s41380-022-01454-5. Epub 2022 Feb 23. PubMed
A genome-scale CRISPR screen reveals PRMT1 as a critical regulator of androgen receptor signaling in prostate cancer. Tang S, Sethunath V, Metaferia NY, Nogueira MF, Gallant DS, Garner ER, Lairson LA, Penney CM, Li J, Gelbard MK, Alaiwi SA, Seo JH, Hwang JH, Strathdee CA, Baca SC, AbuHammad S, Zhang X, Doench JG, Hahn WC, Takeda DY, Freedman ML, Choi PS, Viswanathan SR. Cell Rep. 2022 Feb 22;38(8):110417. doi: 10.1016/j.celrep.2022.110417. PubMed
Regulation of influenza A virus infection by Lnc-PINK1-2:5. Pushparaj S, Zhu Z, Huang C, More S, Liang Y, Lin K, Vaddadi K, Liu L. J Cell Mol Med. 2022 Apr;26(8):2285-2298. doi: 10.1111/jcmm.17249. Epub 2022 Feb 24. PubMed
Dissecting Molecular Phenotypes Through FACS-Based Pooled CRISPR Screens. Genolet O, Ravid Lustig L, Schulz EG. Methods Mol Biol. 2022;2520:1-24. doi: 10.1007/7651_2021_457. PubMed
Targeted intracellular delivery of Cas13 and Cas9 nucleases using bacterial toxin-based platforms. Tian S, Liu Y, Appleton E, Wang H, Church GM, Dong M. Cell Rep. 2022 Mar 8;38(10):110476. doi: 10.1016/j.celrep.2022.110476. PubMed
Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments. Yiu SPT, Guo R, Zerbe C, Weekes MP, Gewurz BE. Cell Rep. 2022 Mar 8;38(10):110411. doi: 10.1016/j.celrep.2022.110411. PubMed
Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics. Passarelli MC, Pinzaru AM, Asgharian H, Liberti MV, Heissel S, Molina H, Goodarzi H, Tavazoie SF. Nat Cell Biol. 2022 Mar;24(3):307-315. doi: 10.1038/s41556-022-00856-5. Epub 2022 Mar 14. PubMed
Avian influenza viruses suppress innate immunity by inducing trans-transcriptional readthrough via SSU72. Zhao Y, Huang F, Zou Z, Bi Y, Yang Y, Zhang C, Liu Q, Shang D, Yan Y, Ju X, Mei S, Xie P, Li X, Tian M, Tan S, Lu H, Han Z, Liu K, Zhang Y, Liang J, Liang Z, Zhang Q, Chang J, Liu WJ, Feng C, Li T, Zhang MQ, Wang X, Gao GF, Liu Y, Jin N, Jiang C. Cell Mol Immunol. 2022 Jun;19(6):702-714. doi: 10.1038/s41423-022-00843-8. Epub 2022 Mar 24. PubMed
Efficient silencing of hepatitis B virus S gene through CRISPR-mediated base editing. Zhou H, Wang X, Steer CJ, Song G, Niu J. Hepatol Commun. 2022 Jul;6(7):1652-1663. doi: 10.1002/hep4.1933. Epub 2022 Mar 26. PubMed
H3K9 trimethylation in active chromatin restricts the usage of functional CTCF sites in SINE B2 repeats. Gualdrini F, Polletti S, Simonatto M, Prosperini E, Pileri F, Natoli G. Genes Dev. 2022 Apr 1;36(7-8):414-432. doi: 10.1101/gad.349282.121. Epub 2022 Mar 31. PubMed
TOX4 facilitates promoter-proximal pausing and C-terminal domain dephosphorylation of RNA polymerase II in human cells. Liu Z, Wu A, Wu Z, Wang T, Pan Y, Li B, Zhang X, Yu M. Commun Biol. 2022 Apr 1;5(1):300. doi: 10.1038/s42003-022-03214-1. PubMed
Loss of Rnf31 and Vps4b sensitizes pancreatic cancer to T cell-mediated killing. Frey N, Tortola L, Egli D, Janjuha S, Rothgangl T, Marquart KF, Ampenberger F, Kopf M, Schwank G. Nat Commun. 2022 Apr 4;13(1):1804. doi: 10.1038/s41467-022-29412-3. PubMed
Reconstructed glycosylase base editors GBE2.0 with enhanced C-to-G base editing efficiency and purity. Sun N, Zhao D, Li S, Zhang Z, Bi C, Zhang X. Mol Ther. 2022 Jul 6;30(7):2452-2463. doi: 10.1016/j.ymthe.2022.03.023. Epub 2022 Apr 4. PubMed
Functional Genomic Analysis of CDK4 and CDK6 Gene Dependency across Human Cancer Cell Lines. Zhang Z, Golomb L, Meyerson M. Cancer Res. 2022 Jun 6;82(11):2171-2184. doi: 10.1158/0008-5472.CAN-21-2428. PubMed
The nuclear lamina binds the EBV genome during latency and regulates viral gene expression. Caruso LB, Guo R, Keith K, Madzo J, Maestri D, Boyle S, Wasserman J, Kossenkov A, Gewurz BE, Tempera I. PLoS Pathog. 2022 Apr 14;18(4):e1010400. doi: 10.1371/journal.ppat.1010400. eCollection 2022 Apr. PubMed
DENR controls JAK2 translation to induce PD-L1 expression for tumor immune evasion. Chen B, Hu J, Hu X, Chen H, Bao R, Zhou Y, Ye Y, Zhan M, Cai W, Li H, Li HB. Nat Commun. 2022 Apr 19;13(1):2059. doi: 10.1038/s41467-022-29754-y. PubMed
Functional elements of the cis-regulatory lincRNA-p21. Winkler L, Jimenez M, Zimmer JT, Williams A, Simon MD, Dimitrova N. Cell Rep. 2022 Apr 19;39(3):110687. doi: 10.1016/j.celrep.2022.110687. PubMed
N-VEGF, the Autoregulatory Arm of VEGF-A. Katsman M, Azriel A, Horev G, Reizel Y, Levi BZ. Cells. 2022 Apr 11;11(8). pii: cells11081289. doi: 10.3390/cells11081289. PubMed
ZNF117 regulates glioblastoma stem cell differentiation towards oligodendroglial lineage. Liu J, Wang X, Chen AT, Gao X, Himes BT, Zhang H, Chen Z, Wang J, Sheu WC, Deng G, Xiao Y, Zou P, Zhang S, Liu F, Zhu Y, Fan R, Patel TR, Saltzman WM, Zhou J. Nat Commun. 2022 Apr 22;13(1):2196. doi: 10.1038/s41467-022-29884-3. PubMed
Inhibition of pyrimidine biosynthesis targets protein translation in acute myeloid leukemia. So J, Lewis AC, Smith LK, Stanley K, Franich R, Yoannidis D, Pijpers L, Dominguez P, Hogg SJ, Vervoort SJ, Brown FC, Johnstone RW, McDonald G, Ulanet DB, Murtie J, Gruber E, Kats LM. EMBO Mol Med. 2022 Jul 7;14(7):e15203. doi: 10.15252/emmm.202115203. Epub 2022 May 6. PubMed
The Chd4 subunit of the NuRD complex regulates Pdx1-controlled genes involved in beta-cell function. Davidson RK, Weaver SA, Casey N, Kanojia S, Hogarth E, Schneider Aguirre R, Sims EK, Evans-Molina C, Spaeth JM. J Mol Endocrinol. 2022 Jun 14;69(2):329-341. doi: 10.1530/JME-22-0011. PubMed
HSP90 N-terminal inhibitors target oncoprotein MORC2 for autophagic degradation and suppress MORC2-driven breast cancer progression. Yang F, Sun R, Hou Z, Zhang FL, Xiao Y, Yang YS, Yang SY, Xie YF, Liu YY, Luo C, Liu GY, Shao ZM, Li DQ. Clin Transl Med. 2022 May;12(5):e825. doi: 10.1002/ctm2.825. PubMed
RNA splicing is a key mediator of tumour cell plasticity and a therapeutic vulnerability in colorectal cancer. Hall AE, Pohl SO, Cammareri P, Aitken S, Younger NT, Raponi M, Billard CV, Carrancio AB, Bastem A, Freile P, Haward F, Adams IR, Caceres JF, Preyzner P, von Kriegsheim A, Dunlop MG, Din FV, Myant KB. Nat Commun. 2022 May 19;13(1):2791. doi: 10.1038/s41467-022-30489-z. PubMed
A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination. Zhao J, Tian S, Guo Q, Bao K, Yu G, Wang X, Shen X, Zhang J, Chen J, Yang Y, Liu L, Li X, Hao J, Yang N, Liu Z, Ai D, Yang J, Zhu Y, Yao Z, Ma S, Zhang K, Shi L. Mol Cell. 2022 Jul 21;82(14):2571-2587.e9. doi: 10.1016/j.molcel.2022.04.031. Epub 2022 May 20. PubMed
Inhibition of nonhomologous end joining-mediated DNA repair enhances anti-HBV CRISPR therapy. Murai K, Kodama T, Hikita H, Shimoda A, Fukuoka M, Fukutomi K, Shigeno S, Shiode Y, Motooka D, Higuchi Y, Miyakawa K, Suemizu H, Ryo A, Tahata Y, Makino Y, Yamada R, Sakamori R, Tatsumi T, Takehara T. Hepatol Commun. 2022 Sep;6(9):2474-2487. doi: 10.1002/hep4.2014. Epub 2022 May 24. PubMed
DELETE_A dual conditional CRISPR-Cas9 system to activate gene editing and reduce off-target effects in human stem cells. Park SB, Uchida T, Tilson S, Hu Z, Ma CD, Leek M, Eichner M, Hong SG, Liang TJ. Mol Ther Nucleic Acids. 2022 Apr 27;28:656-669. doi: 10.1016/j.omtn.2022.04.013. eCollection 2022 Jun 14. PubMed
Loss of SP-A in the Lung Exacerbates Pulmonary Fibrosis. Kim K, Shin D, Lee G, Bae H. Int J Mol Sci. 2022 May 10;23(10). pii: ijms23105292. doi: 10.3390/ijms23105292. PubMed
Histone demethylase KDM4C is a functional dependency in JAK2-mutated neoplasms. Ernst P, Schnoder TM, Huber N, Perner F, Jayavelu AK, Eifert T, Hsu CJ, Tubio-Santamaria N, Crodel CC, Ungelenk M, Hubner CA, Clement JH, Hochhaus A, Heidel FH. Leukemia. 2022 Jul;36(7):1843-1849. doi: 10.1038/s41375-022-01611-3. Epub 2022 Jun 2. PubMed
Diterpenoid Vinigrol specifically activates ATF4/DDIT3-mediated PERK arm of unfolded protein response to drive non-apoptotic death of breast cancer cells. Wei W, Li Y, Wang C, Gao S, Zhao Y, Yang Z, Wang H, Gao Z, Jiang Y, He Y, Zhao L, Gao H, Yao X, Hu Y. Pharmacol Res. 2022 Aug;182:106285. doi: 10.1016/j.phrs.2022.106285. Epub 2022 Jun 1. PubMed
A CRISPR screen identifies redox vulnerabilities for KEAP1/NRF2 mutant non-small cell lung cancer. Jiang C, Ward NP, Prieto-Farigua N, Kang YP, Thalakola A, Teng M, DeNicola GM. Redox Biol. 2022 Aug;54:102358. doi: 10.1016/j.redox.2022.102358. Epub 2022 Jun 2. PubMed
An in-library ligation strategy and its application in CRISPR/Cas9 screening of high-order gRNA combinations. Lu Z, Ni K, Wang Y, Zhou Y, Li Y, Yan J, Song Q, Liu M, Xu Y, Yu Z, Guo T, Ma L. Nucleic Acids Res. 2022 Jun 24;50(11):6575-6586. doi: 10.1093/nar/gkac458. PubMed

Associated Plasmids
Identification of mechanism of cancer-cell-specific reactivation of hTERT offers therapeutic opportunities for blocking telomerase specifically in human colorectal cancer. Akincilar SC, Chua JYH, Ng QF, Chan CHT, Eslami-S Z, Chen K, Low JL, Arumugam S, Aswad L, Chua C, Tan IB, DasGupta R, Fullwood MJ, Tergaonkar V. Nucleic Acids Res. 2022 Jun 14. pii: 6605892. doi: 10.1093/nar/gkac479. PubMed
Single-nucleus profiling of human dilated and hypertrophic cardiomyopathy. Chaffin M, Papangeli I, Simonson B, Akkad AD, Hill MC, Arduini A, Fleming SJ, Melanson M, Hayat S, Kost-Alimova M, Atwa O, Ye J, Bedi KC Jr, Nahrendorf M, Kaushik VK, Stegmann CM, Margulies KB, Tucker NR, Ellinor PT. Nature. 2022 Aug;608(7921):174-180. doi: 10.1038/s41586-022-04817-8. Epub 2022 Jun 22. PubMed
Loss of Stathmin-2, a hallmark of TDP-43-associated ALS, causes motor neuropathy. Krus KL, Strickland A, Yamada Y, Devault L, Schmidt RE, Bloom AJ, Milbrandt J, DiAntonio A. Cell Rep. 2022 Jun 28;39(13):111001. doi: 10.1016/j.celrep.2022.111001. PubMed
B cell receptor signaling drives APOBEC3 expression via direct enhancer regulation in chronic lymphocytic leukemia B cells. Wang Z, Yan H, Boysen JC, Secreto CR, Tschumper RC, Ali D, Guo Q, Zhong J, Zhou J, Gan H, Yu C, Jelinek DF, Slager SL, Parikh SA, Braggio E, Kay NE. Blood Cancer J. 2022 Jul 1;12(7):99. doi: 10.1038/s41408-022-00690-w. PubMed
Epithelial HNF4A shapes the intraepithelial lymphocyte compartment via direct regulation of immune signaling molecules. Lei X, Ketelut-Carneiro N, Shmuel-Galia L, Xu W, Wilson R, Vierbuchen T, Chen Y, Reboldi A, Kang J, Edelblum KL, Ward D, Fitzgerald KA. J Exp Med. 2022 Aug 1;219(8):e20212563. doi: 10.1084/jem.20212563. Epub 2022 Jul 6. PubMed
PHF20 is crucial for epigenetic control of starvation-induced autophagy through enhancer activation. Park SW, Kim J, Oh S, Lee J, Cha J, Lee HS, Kim KI, Park D, Baek SH. Nucleic Acids Res. 2022 Aug 12;50(14):7856-7872. doi: 10.1093/nar/gkac584. PubMed
Structural basis for acyl chain control over glycosphingolipid sorting and vesicular trafficking. Schmieder SS, Tatituri R, Anderson M, Kelly K, Lencer WI. Cell Rep. 2022 Jul 12;40(2):111063. doi: 10.1016/j.celrep.2022.111063. PubMed
RNA-binding protein ELAVL4/HuD ameliorates Alzheimer's disease-related molecular changes in human iPSC-derived neurons. van der Linden RJ, Gerritsen JS, Liao M, Widomska J, Pearse RV 2nd, White FM, Franke B, Young-Pearse TL, Poelmans G. Prog Neurobiol. 2022 Oct;217:102316. doi: 10.1016/j.pneurobio.2022.102316. Epub 2022 Jul 14. PubMed
Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs. Rebendenne A, Roy P, Bonaventure B, Chaves Valadao AL, Desmarets L, Arnaud-Arnould M, Rouille Y, Tauziet M, Giovannini D, Touhami J, Lee Y, DeWeirdt P, Hegde M, Urbach S, Koulali KE, de Gracia FG, McKellar J, Dubuisson J, Wencker M, Belouzard S, Moncorge O, Doench JG, Goujon C. Nat Genet. 2022 Aug;54(8):1090-1102. doi: 10.1038/s41588-022-01110-2. Epub 2022 Jul 25. PubMed
Distinct, opposing functions for CFIm59 and CFIm68 in mRNA alternative polyadenylation of Pten and in the PI3K/Akt signalling cascade. Tseng HW, Mota-Sydor A, Leventis R, Jovanovic P, Topisirovic I, Duchaine TF. Nucleic Acids Res. 2022 Sep 9;50(16):9397-9412. doi: 10.1093/nar/gkac704. PubMed
Cytoplasmic keratins couple with and maintain nuclear envelope integrity in colonic epithelial cells. Stenvall CA, Nystrom JH, Butler-Hallissey C, Jansson T, Heikkila TRH, Adam SA, Foisner R, Goldman RD, Ridge KM, Toivola DM. Mol Biol Cell. 2022 Nov 1;33(13):ar121. doi: 10.1091/mbc.E20-06-0387. Epub 2022 Aug 24. PubMed
Integrated multi-omics analyses identify key anti-viral host factors and pathways controlling SARS-CoV-2 infection. Hou J, Wei Y, Zou J, Jaffery R, Liang S, Zheng C, Chen K, Shi PY, Chen Y, Xie X, Peng W. Res Sq. 2022 Aug 15:rs.3.rs-1910932. doi: 10.21203/rs.3.rs-1910932/v1. Preprint. PubMed
Role of redox-sensitive catalytic interaction with ADAM10 in mutant-selective extracellular shedding of prion protein. Shin Y, Jo KS, Shin M, Lee D, Yeo H, Song Y, Kang SW. Redox Biol. 2022 Oct;56:102456. doi: 10.1016/j.redox.2022.102456. Epub 2022 Aug 28. PubMed
ABL kinases regulate translation in HER2+ cells through Y-box-binding protein 1 to facilitate colonization of the brain. McKernan CM, Khatri A, Hannigan M, Child J, Chen Q, Mayro B, Snyder D, Nicchitta CV, Pendergast AM. Cell Rep. 2022 Aug 30;40(9):111268. doi: 10.1016/j.celrep.2022.111268. PubMed
New regulators of the tetracycline-inducible gene expression system identified by chemical and genetic screens. Colicchia V, Haggblad M, Sirozh O, Porebski B, Balan M, Li X, Lidemalm L, Carreras-Puigvert J, Huhn D, Fernandez-Capetillo O. FEBS Open Bio. 2022 Oct;12(10):1896-1908. doi: 10.1002/2211-5463.13482. Epub 2022 Sep 11. PubMed
A translational genomics approach identifies IL10RB as the top candidate gene target for COVID-19 susceptibility. Voloudakis G, Vicari JM, Venkatesh S, Hoffman GE, Dobrindt K, Zhang W, Beckmann ND, Higgins CA, Argyriou S, Jiang S, Hoagland D, Gao L, Corvelo A, Cho K, Lee KM, Bian J, Lee JS, Iyengar SK, Luoh SW, Akbarian S, Striker R, Assimes TL, Schadt EE, Lynch JA, Merad M, tenOever BR, Charney AW, Brennand KJ, Fullard JF, Roussos P. NPJ Genom Med. 2022 Sep 5;7(1):52. doi: 10.1038/s41525-022-00324-x. PubMed
Epigenetic activation of the FLT3 gene by ZNF384 fusion confers a therapeutic susceptibility in acute lymphoblastic leukemia. Zhao X, Wang P, Diedrich JD, Smart B, Reyes N, Yoshimura S, Zhang J, Yang W, Barnett K, Xu B, Li Z, Huang X, Yu J, Crews K, Yeoh AEJ, Konopleva M, Wei CL, Pui CH, Savic D, Yang JJ. Nat Commun. 2022 Sep 14;13(1):5401. doi: 10.1038/s41467-022-33143-w. PubMed
Double-strand break toxicity is chromatin context independent. Friskes A, Koob L, Krenning L, Severson TM, Koeleman ES, Vergara X, Schubert M, van den Berg J, Evers B, Manjon AG, Joosten S, Kim Y, Zwart W, Medema RH. Nucleic Acids Res. 2022 Sep 23;50(17):9930-9947. doi: 10.1093/nar/gkac758. PubMed
The DEAD box RNA helicase DDX42 is an intrinsic inhibitor of positive-strand RNA viruses. Bonaventure B, Rebendenne A, Chaves Valadao AL, Arnaud-Arnould M, Gracias S, Garcia de Gracia F, McKellar J, Labaronne E, Tauziet M, Vivet-Boudou V, Bernard E, Briant L, Gros N, Djilli W, Courgnaud V, Parrinello H, Rialle S, Blaise M, Lacroix L, Lavigne M, Paillart JC, Ricci EP, Schulz R, Jouvenet N, Moncorge O, Goujon C. EMBO Rep. 2022 Nov 7;23(11):e54061. doi: 10.15252/embr.202154061. Epub 2022 Sep 26. PubMed
TP53 mutations and RNA-binding protein MUSASHI-2 drive resistance to PRMT5-targeted therapy in B-cell lymphoma. Erazo T, Evans CM, Zakheim D, Chu KL, Refermat AY, Asgari Z, Yang X, Da Silva Ferreira M, Mehta S, Russo MV, Knezevic A, Zhang XP, Chen Z, Fennell M, Garippa R, Seshan V, de Stanchina E, Barbash O, Batlevi CL, Leslie CS, Melnick AM, Younes A, Kharas MG. Nat Commun. 2022 Sep 27;13(1):5676. doi: 10.1038/s41467-022-33137-8. PubMed
The phospholipid flippase ATP8B1 is required for lysosomal fusion in macrophages. Gomez-Mellado VE, Ho-Mok KS, van der Mark VA, van der Wel NN, Grootemaat AE, Verhoeven AJ, Elferink RPJO, Paulusma CC. Cell Biochem Funct. 2022 Dec;40(8):914-925. doi: 10.1002/cbf.3752. Epub 2022 Sep 28. PubMed
Enzyme-free targeted DNA demethylation using CRISPR-dCas9-based steric hindrance to identify DNA methylation marks causal to altered gene expression. Sapozhnikov DM, Szyf M. Nat Protoc. 2022 Dec;17(12):2840-2881. doi: 10.1038/s41596-022-00741-3. Epub 2022 Oct 7. PubMed
DNA damage contributes to age-associated differences in SARS-CoV-2 infection. Jin R, Niu C, Wu F, Zhou S, Han T, Zhang Z, Li E, Zhang X, Xu S, Wang J, Tian S, Chen W, Ye Q, Cao C, Cheng L. Aging Cell. 2022 Dec;21(12):e13729. doi: 10.1111/acel.13729. Epub 2022 Oct 18. PubMed
A Genome-Wide CRISPR-Cas9 Loss-of-Function Screening to Identify Host Restriction Factors Modulating Oncolytic Virotherapy. Muscolini M, Hiscott J, Tassone E. Methods Mol Biol. 2023;2589:379-399. doi: 10.1007/978-1-0716-2788-4_25. PubMed
Dissecting super-enhancer driven transcriptional dependencies reveals novel therapeutic strategies and targets for group 3 subtype medulloblastoma. Li M, Han Y, Wang C, Kang W, Jiang W, Zhang L, Tang Y. J Exp Clin Cancer Res. 2022 Oct 22;41(1):311. doi: 10.1186/s13046-022-02506-y. PubMed
Oxidized thioredoxin-1 restrains the NLRP1 inflammasome. Ball DP, Tsamouri LP, Wang AE, Huang HC, Warren CD, Wang Q, Edmondson IH, Griswold AR, Rao SD, Johnson DC, Bachovchin DA. Sci Immunol. 2022 Nov 11;7(77):eabm7200. doi: 10.1126/sciimmunol.abm7200. Epub 2022 Nov 4. PubMed
HNRNPU promotes the progression of triple-negative breast cancer via RNA transcription and alternative splicing mechanisms. Han BY, Liu Z, Hu X, Ling H. Cell Death Dis. 2022 Nov 8;13(11):940. doi: 10.1038/s41419-022-05376-6. PubMed
Systematic discovery and functional dissection of enhancers needed for cancer cell fitness and proliferation. Chen PB, Fiaux PC, Zhang K, Li B, Kubo N, Jiang S, Hu R, Rooholfada E, Wu S, Wang M, Wang W, McVicker G, Mischel PS, Ren B. Cell Rep. 2022 Nov 8;41(6):111630. doi: 10.1016/j.celrep.2022.111630. PubMed
Identification of purine biosynthesis as an NADH-sensing pathway to mediate energy stress. Yang R, Yang C, Ma L, Zhao Y, Guo Z, Niu J, Chu Q, Ma Y, Li B. Nat Commun. 2022 Nov 17;13(1):7031. doi: 10.1038/s41467-022-34850-0. PubMed

Associated Plasmids
Extensive androgen receptor enhancer heterogeneity in primary prostate cancers underlies transcriptional diversity and metastatic potential. Kneppers J, Severson TM, Siefert JC, Schol P, Joosten SEP, Yu IPL, Huang CF, Morova T, Altintas UB, Giambartolomei C, Seo JH, Baca SC, Carneiro I, Emberly E, Pasaniuc B, Jeronimo C, Henrique R, Freedman ML, Wessels LFA, Lack NA, Bergman AM, Zwart W. Nat Commun. 2022 Nov 30;13(1):7367. doi: 10.1038/s41467-022-35135-2. PubMed
Whole genome sequencing identifies structural variants contributing to hematologic traits in the NHLBI TOPMed program. Wheeler MM, Stilp AM, Rao S, Halldorsson BV, Beyter D, Wen J, Mihkaylova AV, McHugh CP, Lane J, Jiang MZ, Raffield LM, Jun G, Sedlazeck FJ, Metcalf G, Yao Y, Bis JB, Chami N, de Vries PS, Desai P, Floyd JS, Gao Y, Kammers K, Kim W, Moon JY, Ratan A, Yanek LR, Almasy L, Becker LC, Blangero J, Cho MH, Curran JE, Fornage M, Kaplan RC, Lewis JP, Loos RJF, Mitchell BD, Morrison AC, Preuss M, Psaty BM, Rich SS, Rotter JI, Tang H, Tracy RP, Boerwinkle E, Abecasis GR, Blackwell TW, Smith AV, Johnson AD, Mathias RA, Nickerson DA, Conomos MP, Li Y, Thornorsteinsdottir U, Magnusson MK, Stefansson K, Pankratz ND, Bauer DE, Auer PL, Reiner AP. Nat Commun. 2022 Dec 8;13(1):7592. doi: 10.1038/s41467-022-35354-7. PubMed
Workflow for Performing Genetic Manipulation in Human Trophoblast Stem Cells Using CRISPR/Cas9 Technology. Wu H, Wang Y, Wang H. Methods Mol Biol. 2022 Dec 15. doi: 10.1007/7651_2022_464. PubMed
PAX5 epigenetically orchestrates CD58 transcription and modulates blinatumomab response in acute lymphoblastic leukemia. Li Y, Moriyama T, Yoshimura S, Zhao X, Li Z, Yang X, Paietta E, Litzow MR, Konopleva M, Yu J, Inaba H, Ribeiro RC, Pui CH, Yang JJ. Sci Adv. 2022 Dec 14;8(50):eadd6403. doi: 10.1126/sciadv.add6403. Epub 2022 Dec 14. PubMed
SMAD9-MYCN positive feedback loop represents a unique dependency for MYCN-amplified neuroblastoma. Tan K, Mo J, Li M, Dong Y, Han Y, Sun X, Ma Y, Zhu K, Wu W, Lu L, Liu J, Zhao K, Zhang L, Tang Y, Lv Z. J Exp Clin Cancer Res. 2022 Dec 20;41(1):352. doi: 10.1186/s13046-022-02563-3. PubMed
Deletion of Bak1 alleviates microglial necroptosis and neuroinflammation after experimental subarachnoid hemorrhage. Qiu X, Tao Q, Zhang L, Kuang C, Xie Y, Zhang L, Yin S, Peng J, Jiang Y. J Neurochem. 2023 Mar;164(6):829-846. doi: 10.1111/jnc.15751. Epub 2023 Jan 7. PubMed
Thymidine nucleotide metabolism controls human telomere length. Mannherz W, Agarwal S. Nat Genet. 2023 Apr;55(4):568-580. doi: 10.1038/s41588-023-01339-5. Epub 2023 Mar 23. PubMed
TNRC18 engages H3K9me3 to mediate silencing of endogenous retrotransposons. Zhao S, Lu J, Pan B, Fan H, Byrum SD, Xu C, Kim A, Guo Y, Kanchi KL, Gong W, Sun T, Storey AJ, Burkholder NT, Mackintosh SG, Kuhlers PC, Edmondson RD, Strahl BD, Diao Y, Tackett AJ, Raab JR, Cai L, Song J, Wang GG. Nature. 2023 Nov;623(7987):633-642. doi: 10.1038/s41586-023-06688-z. Epub 2023 Nov 8. PubMed
Epstein-Barr virus induces germinal center light zone chromatin architecture and promotes survival through enhancer looping at the BCL2A1 locus. Dai J, SoRelle ED, Heckenberg E, Song L, Cable JM, Crawford GE, Luftig MA. mBio. 2024 Jan 16;15(1):e0244423. doi: 10.1128/mbio.02444-23. Epub 2023 Dec 7. PubMed
Epstein-Barr Virus miR-BARTs 7 and 9 modulate viral cycle, cell proliferation, and proteomic profiles in Burkitt lymphoma. Caetano BFR, Rocha VL, Rossini BC, Dos Santos LD, Elgui De Oliveira D. Tumour Virus Res. 2024 Jun;17:200276. doi: 10.1016/j.tvr.2023.200276. Epub 2023 Dec 29. PubMed
Germinal Center Cytokines Driven Epigenetic Control of Epstein-Barr Virus Latency Gene Expression. Yifei L, Jinjie Y, Beri NR, Roth LG, Ethel C, Benjamin E G. bioRxiv [Preprint]. 2024 Jan 3:2024.01.02.573986. doi: 10.1101/2024.01.02.573986. PubMed
Genome-wide loss-of-function genetic screen identifies INSIG2 as the vulnerability of hepatitis B virus-integrated hepatoma cells. Fukuoka M, Kodama T, Murai K, Hikita H, Sometani E, Sung J, Shimoda A, Shigeno S, Motooka D, Nishio A, Furuta K, Tatsumi T, Yusa K, Takehara T. Cancer Sci. 2024 Mar;115(3):859-870. doi: 10.1111/cas.16070. Epub 2024 Jan 29. PubMed
3D genomic analysis reveals novel enhancer-hijacking caused by complex structural alterations that drive oncogene overexpression. Mortenson KL, Dawes C, Wilson ER, Patchen NE, Johnson HE, Gertz J, Bailey SD, Liu Y, Varley KE, Zhang X. bioRxiv [Preprint]. 2024 Jun 21:2024.01.23.576965. doi: 10.1101/2024.01.23.576965. PubMed
Genome-scale pan-cancer interrogation of lncRNA dependencies using CasRx. Montero JJ, Trozzo R, Sugden M, Ollinger R, Belka A, Zhigalova E, Waetzig P, Engleitner T, Schmidt-Supprian M, Saur D, Rad R. Nat Methods. 2024 Feb 26. doi: 10.1038/s41592-024-02190-0. PubMed

Associated Plasmids
In vivo CRISPR screening directly targeting testicular cells. Noguchi Y, Onodera Y, Miyamoto T, Maruoka M, Kosako H, Suzuki J. Cell Genom. 2024 Mar 13;4(3):100510. doi: 10.1016/j.xgen.2024.100510. Epub 2024 Mar 5. PubMed
Repeat DNA methylation is modulated by adherens junction signaling. Brenner LM, Meyer F, Yang H, Kohler AR, Bashtrykov P, Guo M, Jeltsch A, Lungu C, Olayioye MA. Commun Biol. 2024 Mar 7;7(1):286. doi: 10.1038/s42003-024-05990-4. PubMed
PRPF19 mRNA Encodes a Small Open Reading Frame That Is Important for Viability of Human Cells. Shepelev NM, Kurochkina AO, Dontsova OA, Rubtsova MP. Dokl Biochem Biophys. 2024 Apr;515(1):41-47. doi: 10.1134/S1607672923700722. Epub 2024 Mar 12. PubMed
NFATC2IP is a mediator of SUMO-dependent genome integrity. Cho T, Hoeg L, Setiaputra D, Durocher D. Genes Dev. 2024 Apr 17;38(5-6):233-252. doi: 10.1101/gad.350914.123. PubMed

Associated Plasmids
Targeting of vulnerabilities of drug-tolerant persisters identified through functional genetics delays tumor relapse. Chen M, Mainardi S, Lieftink C, Velds A, de Rink I, Yang C, Kuiken HJ, Morris B, Edwards F, Jochems F, van Tellingen O, Boeije M, Proost N, Jansen RA, Qin S, Jin H, Koen van der Mijn JC, Schepers A, Venkatesan S, Qin W, Beijersbergen RL, Wang L, Bernards R. Cell Rep Med. 2024 Mar 19;5(3):101471. doi: 10.1016/j.xcrm.2024.101471. PubMed
Pooled CRISPR screening of high-content cellular phenotypes using ghost cytometry. Tsubouchi A, An Y, Kawamura Y, Yanagihashi Y, Nakayama H, Murata Y, Teranishi K, Ishiguro S, Aburatani H, Yachie N, Ota S. Cell Rep Methods. 2024 Mar 25;4(3):100737. doi: 10.1016/j.crmeth.2024.100737. PubMed
CRISPR/Cas9 targeting of passenger single nucleotide variants in haploinsufficient or essential genes expands cancer therapy prospects. Kim H, Han JH, Kim H, Kim M, Jo SI, Lee N, Cha S, Oh MJ, Choi G, Kim HS. Sci Rep. 2024 Mar 28;14(1):7436. doi: 10.1038/s41598-024-58094-8. PubMed
The SPATA5-SPATA5L1 ATPase complex directs replisome proteostasis to ensure genome integrity. Krishnamoorthy V, Foglizzo M, Dilley RL, Wu A, Datta A, Dutta P, Campbell LJ, Degtjarik O, Musgrove LJ, Calabrese AN, Zeqiraj E, Greenberg RA. Cell. 2024 Apr 25;187(9):2250-2268.e31. doi: 10.1016/j.cell.2024.03.002. Epub 2024 Mar 29. PubMed
Deletion of the TMEM30A gene enables leukemic cell evasion of NK cell cytotoxicity. Kristenson L, Badami C, Ljungberg A, Islamagic E, Tian Y, Xie G, Hussein BA, Pesce S, Tang KW, Thoren FB. Proc Natl Acad Sci U S A. 2024 Apr 9;121(15):e2316447121. doi: 10.1073/pnas.2316447121. Epub 2024 Apr 1. PubMed
The PRC2.1 Subcomplex Opposes G1 Progression through Regulation of CCND1 and CCND2. Longhurst AD, Wang K, Suresh HG, Ketavarapu M, Ward HN, Jones IR, Narayan V, Hundley FV, Hassan AZ, Boone C, Myers CL, Shen Y, Ramani V, Andrews BJ, Toczyski DP. bioRxiv [Preprint]. 2024 Mar 19:2024.03.18.585604. doi: 10.1101/2024.03.18.585604. PubMed
BLM helicase unwinds lagging strand substrates to assemble the ALT telomere damage response. Jiang H, Zhang T, Kaur H, Shi T, Krishnan A, Kwon Y, Sung P, Greenberg RA. Mol Cell. 2024 May 2;84(9):1684-1698.e9. doi: 10.1016/j.molcel.2024.03.011. Epub 2024 Apr 8. PubMed
Genome-wide screening identifies Trim33 as an essential regulator of dendritic cell differentiation. Tiniakou I, Hsu PF, Lopez-Zepeda LS, Garipler G, Esteva E, Adams NM, Jang G, Soni C, Lau CM, Liu F, Khodadadi-Jamayran A, Rodrick TC, Jones D, Tsirigos A, Ohler U, Bedford MT, Nimer SD, Kaartinen V, Mazzoni EO, Reizis B. Sci Immunol. 2024 Apr 12;9(94):eadi1023. doi: 10.1126/sciimmunol.adi1023. Epub 2024 Apr 12. PubMed
Functional genomics identifies N-acetyllactosamine extension of complex N-glycans as a mechanism to evade lysis by natural killer cells. Zhuang X, Woods J, Ji Y, Scheich S, Mo F, Rajagopalan S, Coulibaly ZA, Voss M, Urlaub H, Staudt LM, Pan KT, Long EO. Cell Rep. 2024 Apr 23;43(4):114105. doi: 10.1016/j.celrep.2024.114105. Epub 2024 Apr 14. PubMed
Frequent loss of FAM126A expression in colorectal cancer results in selective FAM126B dependency. Li S, Han T. iScience. 2024 Mar 29;27(5):109646. doi: 10.1016/j.isci.2024.109646. eCollection 2024 May 17. PubMed
IGSF8 is an innate immune checkpoint and cancer immunotherapy target. Li Y, Wu X, Sheng C, Liu H, Liu H, Tang Y, Liu C, Ding Q, Xie B, Xiao X, Zheng R, Yu Q, Guo Z, Ma J, Wang J, Gao J, Tian M, Wang W, Zhou J, Jiang L, Gu M, Shi S, Paull M, Yang G, Yang W, Landau S, Bao X, Hu X, Liu XS, Xiao T. Cell. 2024 May 23;187(11):2703-2716.e23. doi: 10.1016/j.cell.2024.03.039. Epub 2024 Apr 23. PubMed
Epstein-Barr-Virus-Driven Cardiolipin Synthesis Sustains Metabolic Remodeling During B-cell Lymphomagenesis. You H, Havey L, Li Z, Asara J, Guo R. Res Sq [Preprint]. 2024 Apr 8:rs.3.rs-4013392. doi: 10.21203/rs.3.rs-4013392/v1. PubMed
Positive selection CRISPR screens reveal a druggable pocket in an oligosaccharyltransferase required for inflammatory signaling to NF-kappaB. Lampson BL, Ramiotarez AS, Baro M, He L, Hegde M, Koduri V, Pfaff JL, Hanna RE, Kowal J, Shirole NH, He Y, Doench JG, Contessa JN, Locher KP, Kaelin WG Jr. Cell. 2024 Apr 25;187(9):2209-2223.e16. doi: 10.1016/j.cell.2024.03.022. PubMed
Ribosome subunit attrition and activation of the p53-MDM4 axis dominate the response of MLL-rearranged cancer cells to WDR5 WIN site inhibition. Howard GC, Wang J, Rose KL, Jones C, Patel P, Tsui T, Florian AC, Vlach L, Lorey SL, Grieb BC, Smith BN, Slota MJ, Reynolds EM, Goswami S, Savona MR, Mason FM, Lee T, Fesik S, Liu Q, Tansey WP. Elife. 2024 Apr 29;12:RP90683. doi: 10.7554/eLife.90683. PubMed
Germinal center cytokine driven epigenetic control of Epstein-Barr virus latency gene expression. Liao Y, Yan J, Beri NR, Giulino-Roth L, Cesarman E, Gewurz BE. PLoS Pathog. 2024 Apr 29;20(4):e1011939. doi: 10.1371/journal.ppat.1011939. eCollection 2024 Apr. PubMed
Modulation of the microhomology-mediated end joining pathway suppresses large deletions and enhances homology-directed repair following CRISPR-Cas9-induced DNA breaks. Yuan B, Bi C, Tian Y, Wang J, Jin Y, Alsayegh K, Tehseen M, Yi G, Zhou X, Shao Y, Romero FV, Fischle W, Izpisua Belmonte JC, Hamdan S, Huang Y, Li M. BMC Biol. 2024 Apr 29;22(1):101. doi: 10.1186/s12915-024-01896-z. PubMed
Germline cis variant determines epigenetic regulation of the anti-cancer drug metabolism gene dihydropyrimidine dehydrogenase (DPYD). Zhang T, Ambrodji A, Huang H, Bouchonville KJ, Etheridge AS, Schmidt RE, Bembenek BM, Temesgen ZB, Wang Z, Innocenti F, Stroka D, Diasio RB, Largiader CR, Offer SM. Elife. 2024 Apr 30;13:RP94075. doi: 10.7554/eLife.94075. PubMed
GPATCH8 modulates mutant SF3B1 mis-splicing and pathogenicity in hematologic malignancies. Benbarche S, Pineda JMB, Galvis LB, Biswas J, Liu B, Wang E, Zhang Q, Hogg SJ, Lyttle K, Dahi A, Lewis AM, Sarchi M, Rahman J, Fox N, Ai Y, Mehta S, Garippa R, Ortiz-Pacheco J, Li Z, Monetti M, Stanley RF, Doulatov S, Bradley RK, Abdel-Wahab O. Mol Cell. 2024 May 16;84(10):1886-1903.e10. doi: 10.1016/j.molcel.2024.04.006. Epub 2024 Apr 29. PubMed
Long-term expandable mouse and human-induced nephron progenitor cells enable kidney organoid maturation and modeling of plasticity and disease. Huang B, Zeng Z, Kim S, Fausto CC, Koppitch K, Li H, Li Z, Chen X, Guo J, Zhang CC, Ma T, Medina P, Schreiber ME, Xia MW, Vonk AC, Xiang T, Patel T, Li Y, Parvez RK, Der B, Chen JH, Liu Z, Thornton ME, Grubbs BH, Diao Y, Dou Y, Gnedeva K, Ying Q, Pastor-Soler NM, Fei T, Hallows KR, Lindstrom NO, McMahon AP, Li Z. Cell Stem Cell. 2024 Jun 6;31(6):921-939.e17. doi: 10.1016/j.stem.2024.04.002. Epub 2024 Apr 30. PubMed
Identification of WNK1 as a therapeutic target to suppress IgH/MYC expression in multiple myeloma. Ye T, Mishra AK, Banday S, Li R, Hu K, Coleman MM, Shan Y, Chowdhury SR, Zhou L, Pak ML, Simone TM, Malonia SK, Zhu LJ, Kelliher MA, Green MR. Cell Rep. 2024 May 28;43(5):114211. doi: 10.1016/j.celrep.2024.114211. Epub 2024 May 8. PubMed
An ATP13A1-assisted topogenesis pathway for folding multi-spanning membrane proteins. Ji J, Cui MK, Zou R, Wu MZ, Ge MX, Li J, Zhang ZR. Mol Cell. 2024 May 16;84(10):1917-1931.e15. doi: 10.1016/j.molcel.2024.04.010. Epub 2024 May 8. PubMed
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