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Addgene

lentiCas9-Blast Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes:

Uncut;
KpnI;
NdeI;
KpnI & NdeI;
SapI.

We expected the following band sizes for this digest:

Uncut - 12.9kb;
KpnI - 12.9kb;
NdeI - 12.9kb;
KpnI & NdeI - 7.8kb + 5.0kb;
SapI - 9.5kb + 3.4lb.

We see the expected pattern.