lentiCas9-Blast Notes
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes:
Uncut;
KpnI;
NdeI;
KpnI & NdeI;
SapI.
We expected the following band sizes for this digest:
Uncut - 12.9kb;
KpnI - 12.9kb;
NdeI - 12.9kb;
KpnI & NdeI - 7.8kb + 5.0kb;
SapI - 9.5kb + 3.4lb.
We see the expected pattern.